COMPOSIÇÃO QUÍMICA DE SEMENTES DE TRIGO (Triticum aestivum L.) SUBMETIDAS A ESTRESSE SALINO NA GERMINAÇÃO 1

COMPOSIÇÃO QUÍMICA DE SEMENTES DE TRIGO (Triticum aestivum L.) SUBMETIDAS A ESTRESSE SALINO NA GERMINAÇÃO ¹

RODRIGO NASCIMENTO DA SILVA

, GISELA LOUREIRO DUARTE

, NEI FERNANDES LOPES

, DARIO MUNT DE MORAES

, ANA LÚCIA DE ALMEIDA PEREIRA

RESUMO – O objetivo do experimento foi determinar algumas alterações bioquímicas de sementes GHGXDVFXOWLYDUHVGHWULJR%56H%56VRELQÀXrQFLDGHGLIHUHQWHVQtYHLVGHVDO]HUR HP01D&OQRSURFHVVRGHJHUPLQDomR2HQVDLRIRLUHDOL]DGRSDUDDYDOLDUDOWHUDo}HV QDDWLYLGDGHGDĮDPLODVHHQRVWHRUHVGHDPLGRDo~FDUHVUHGXWRUHVFDUERLGUDWRVVRO~YHLVWRWDLVH SURWHtQDVFDXVDGDVSHORHVWUHVVHVDOLQR$DWLYLGDGHGDDDPLODVHHRVWHRUHVFDUERLGUDWRVVRO~YHLV WRWDLV Do~FDUHV UHGXWRUHV H SURWHtQDV VmR UHGX]LGRV SRUpP R FRQWH~GR GH DPLGR DXPHQWD FRP R LQFUHPHQWR GD VDOLQLGDGH HP DPEDV FXOWLYDUHV GH WULJR (P WRGDV DV FRQFHQWUDo}HV GH 1D&O RV FRPSRQHQWHVTXtPLFRVQDVVHPHQWHVGDFXOWLYDU%56VmRPDLRUHVGRTXHRVGDFY%56 PRVWUDQGRVHUD%56PDLVWROHUDQWHjVDOLQLGDGH

7HUPRV SDUD LQGH[DomR Triticum aestivum L. ĮDPLODVH DPLGR Do~FDUHV SURWHtQDV DOWHUDo}HV ELRTXtPLFDVVDO

&+(0,&$/&20326,7,212):+($7Triticum aestivum /6(('668%0,77('72 6$/,1(675(66

$%675$&7±7KHREMHFWLYHRIWKHH[SHULPHQWZDVWRGHWHUPLQHVRPHELRFKHPLFDOFKDQJHVLQWZR ZKHDWFXOWLYDUV%56DQG%5$XQGHUWKHLQÀXHQFHRIGLIIHUHQWVDOWOHYHOV]HUR DQGP01D&O7KHH[SHULPHQWZDVFDUULHGRXWWRHYDOXDWHWKHHIIHFWRIVDOLQHVWUHVVRQĮDP\ODVH DFWLYLW\DQGVWDUFKVXJDUUHGXFHUVWRWDOVROXEOHFDUERK\GUDWHVDQGSURWHLQFRQWHQWV7KHĮDP\ODVH DFWLYLW\DQGWKHFRQWHQWVRIWRWDOVROXEOHFDUERK\GUDWHVVXJDUUHGXFHUVDQGSURWHLQVGHFUHDVHGEXW VWDUFKFRQWHQWLQFUHDVHGZLWKWKHLQFUHDVHLQVDOLQLW\IRUERWKFXOWLYDUV$WDOO1D&OFRQFHQWUDWLRQV WKHFKHPLFDOFRPSRQHQWVLQWKHVHHGVRIWKH%56FXOWLYDUZHUHKLJKHUWKDQWKHRQHVRIWKH%56 FXOWLYDUVKRZLQJWKDWWKH%56FXOWLYDULVPRUHWROHUDQWWRVDOLQLW\

,QGH[WHUPVTriticum aestivum/ĮDP\ODVHVWDUFKVXJDUVSURWHLQVELRFKHPLFDOFKDQJHVVDOW

1

6XEPHWLGRHP$FHLWRSDUDSXEOLFDomRHP3DUWH GDGLVVHUWDomRGHPHVWUDGRDSUHVHQWDGRj8)3(/56GRSULPHLURDXWRU

2

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GH3HORWDV56&DL[D3RVWDO3oDGHVHWHPEUR 3HORWDV56HPDLOJLVHODGXDUWH#JPDLOFRPEU

Eng

$JU

3URI GR 'HSDUWDPHQWR GH %RWkQLFD ,QVWLWXWR GH %LRORJLD 8QLYHUVLGDGH )HGHUDO GH 3HORWDV &DL[D 3RVWDO 3HORWDV56 HPDLOQHLORSHV#XISHOHGXEU

%LyORJD063URI

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NOTA CIENTÍFICA

INTRODUÇÃO

O aproveitamento de algumas áreas como as de regiões iULGDV H VHPLiULGDV HVEDUUD HP DOJXQV REVWiFXORV FRPR D VDOLQLGDGHHRSRWHQFLDOGHVRGLFLGDGHGDiJXDXWLOL]DGDQD LUULJDomRFXMD¿QDOLGDGHpSURSRUFLRQDUXPLGDGHDGHTXDGD SDUDRFUHVFLPHQWRHRGHVHQYROYLPHQWRGDVSODQWDVYLVDQGR DXPHQWDU D SURGXWLYLGDGH H FRQVHTHQWHPHQWH UHGX]LU RV HIHLWRVGDHVWLDJHP5LEHLURHWDO

$SURSRUomRUHODWLYDGRVFRQVWLWXLQWHVTXtPLFRVFRQWXGR SRGHYDULDUGHDFRUGRFRPDPDQLSXODomRJHQpWLFDSUiWLFDV DJURQ{PLFDV PDQHMR SyVFROKHLWD H GH DUPD]HQDPHQWR idade da semente e tratamento no processo aplicado na SUHSDUDomR GDV VHPHQWHV SDUD R FRQVXPR KXPDQR e LQÀXHQFLDGD DLQGD SHODV FRQGLo}HV GR DPELHQWH D TXH IRUDP VXEPHWLGDV DV SODQWDV TXH DV RULJLQDUDP &DUYDOKR 1DNDJDZD 7DPEpP D VDOLQLGDGH SRGH DOWHUDU RX DIHWDU VLJQL¿FDWLYDPHQWH D FRPSRVLomR TXtPLFD GDV VHPHQWHVSUHMXGLFDQGRRXGL¿FXOWDQGRDJHUPLQDomRDTXDO GHSHQGHSULQFLSDOPHQWHGDVVXEVWkQFLDVGHUHVHUYD

$ VHPHQWH DSUHVHQWD FRPSRVLomR TXtPLFD EDVWDQWH YDULiYHODVVLPFRPRRVGHPDLVyUJmRVGDSODQWD$VHPHQWH SRUVHUXPyUJmRIRUPDGRDR¿QDOGRFLFORYLWDOGDSODQWD FDUDFWHUL]DVH SRU DSUHVHQWDU EDVLFDPHQWH GRLV JUXSRV GH FRPSRQHQWHV TXtPLFRV RV TXH RFRUUHP QRUPDOPHQWH como constituintes em todos os tecidos da planta e os que VmRPDWHULDLVGHUHVHUYD7DLVFRPSRQHQWHVRULJLQDPVHGH HOHPHQWRVDFXPXODGRVHPRXWUDVSDUWHVGDSODQWDTXHSRU WUDQVORFDomRVmRHQYLDGRVDHVVHyUJmRRXIRWRVVLQWHWL]DGRV SRURFDVLmRGDIRUPDomRHGRGHVHQYROYLPHQWRGDVHPHQWH

&DUYDOKR 1DNDJDZD

2FRQKHFLPHQWRGDFRPSRVLomRTXtPLFDpGHLQWHUHVVH SUiWLFRGDWHFQRORJLDGHVHPHQWHSRLVWDQWRRYLJRUFRPRR SRWHQFLDO GH DUPD]HQDPHQWR GH VHPHQWH VmR LQÀXHQFLDGRV SHOR WHRU GRV FRPSRVWRV SUHVHQWHV 6LQFODLU :LW 2XWUR DVSHFWR LPSRUWDQWH GL] UHVSHLWR j LQÀXrQFLD GD FRPSRVLomR TXtPLFD GD VHPHQWH QR JDVWR GDV SODQWDV HP HQHUJLDSDUDSURGX]LODV6LQFODLU :LW

2V KLGUDWRV GH FDUERQR VmR TXDQWLWDWLYDPHQWH RV FRPSRQHQWHV PDLV LPSRUWDQWHV QRV FHUHDLV FRQVWLWXLQGR DSUR[LPDGDPHQWHGDPDWpULDVHFDWRWDOGDVHPHQWHGH WULJRFHYDGDFHQWHLRPLOKRVRUJRHDUUR]HFHUFDGHQD DYHLD1RVFHUHDLVRSULQFLSDOKLGUDWRGHFDUERQRpRDPLGR FRQVWLWXLQGRDSUR[LPDGDPHQWHGDVHPHQWHGHWULJRH GRVHXHQGRVSHUPD'DQWDV 0DULQKR

2 DPLGR QR HQGRVSHUPD GD VHPHQWH p KLGUROLVDGR SHODĮDPLODVHFDXVDQGRDSURGXomRGHPDOWRVHVXEVWUDWR VRO~YHO GLVSRQtYHO SDUD R GHVHQYROYLPHQWR GR HPEULmR $ ĮDPLODVHpXPDGDVHQ]LPDVKLGUROtWLFDVPDLVSURHPLQHQWHV

QDVHPHQWHRTXDOWHPVXDVtQWHVH³GHQRYR´LQGX]LGDSHOD JLEHUHOLQD QD FDPDGD GH DOHXURQD FHUFD GH XP GLD DSyV D DEVRUomRGHiJXD%HZOH\ %ODFN

2XWUR LPSRUWDQWH FRPSRQHQWH TXtPLFR p D SURWHtQD HQFRQWUDGD HP WRGRV RV WHFLGRV GD VHPHQWH HP PDLRUHV FRQFHQWUDo}HVQRHPEULmR

$ FRPSRVLomR TXtPLFDGD VHPHQWH GXUDQWH R SURFHVVR germinativo pode ser alterada conforme as condições DPELHQWHVDTXHDVHPHQWHpVXEPHWLGD2WUDEDOKRWHYHSRU REMHWLYRLGHQWL¿FDUDOJXPDVDOWHUDo}HVELRTXtPLFDVRULXQGDV GDDomRGDVDOLQLGDGHQDJHUPLQDomRGHVHPHQWHVGHWULJR

MATERIAL E MÉTODOS

2H[SHULPHQWRIRLFRQGX]LGRQR/DERUDWyULRGH)LVLRORJLD 9HJHWDO GR 'HSDUWDPHQWR GH %RWkQLFD GD 8QLYHUVLGDGH )HGHUDOGH3HORWDV

Sementes de trigo das cultivares BRS 177 e BRS 179 IRUDP HPEHELGDV SRU XPD KRUD HP VROXo}HV GH FORUHWR GH VyGLR QDV FRQFHQWUDo}HV GH ]HUR H P0 3RVWHULRUPHQWHDVVHPHQWHVIRUDPVXEPHWLGDVjJHUPLQDomR em papel substrato marca germitest umedecido com água GHVWLODGD QD SURSRUomR YH]HV D VXD PDVVD LQLFLDO H mantidas em germinador à temperatura controlada de 25º ±

ž&

Os efeitos da salinidade sobre a atividade total da ĮDPLODVHHRVWHRUHVGHDPLGRFDUERLGUDWRVVRO~YHLVWRWDLV Do~FDUHV UHGXWRUHV H SURWHtQDV IRUDP GHWHUPLQDGRV QRV WHPSRV]HURTXDWURHRLWRGLDVDSyVDJHUPLQDomR'$*

XVDQGRFLQFRVHPHQWHVSDUDRWHPSR]HURHFLQFRSOkQWXODV SDUDTXDWURHRLWR'$*

$ GHWHUPLQDomR GD DWLYLGDGH WRWDO GD ĮDPLODVH IRL HIHWXDGD HP H[WUDWRV VHJXQGR D PHWRGRORJLD HVSHFt¿FD H D OHLWXUD GDV DPRVWUDV HP HVSHFWRIRW{PHWUR D QP

$2$& H RV UHVXOWDGRV H[SUHVVRV HP PJ GH DPLGR KLGUROLVDGRJ

GHVHPHQWHPLQ

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2GHOLQHDPHQWRH[SHULPHQWDOXWLOL]DGRIRLRLQWHLUDPHQWH

FDVXDOL]DGR HP HVTXHPD IDWRULDO [[ VHQGR GXDV

FXOWLYDUHVWUrVpSRFDVGHGHWHUPLQDomRHFLQFRFRQFHQWUDo}HV

VDOLQDVFRPWUrVUHSHWLo}HV$VPpGLDVGRVWUDWDPHQWRVIRUDP

FRPSDUDGDVSHORWHVWHGH7XNH\DGHSUREDELOLGDGHH

GHSRLVVXEPHWHXVHjDQiOLVHGHUHJUHVVmR

RESULTADOS E DISCUSSÃO

$DWLYLGDGHWRWDOGD DDPLODVHGLPLQXLXVLJQL¿FDWLYDPHQWH 3 ” FRP R LQFUHPHQWR GD FRQFHQWUDomR VDOLQD SDUD DPEDV DV FXOWLYDUHV LQGHSHQGHQWHPHQWH GR WHPSR GH GHWHUPLQDomR (QWUHWDQWR D PDLRU DWLYLGDGH HQ]LPiWLFD RFRUUHX DRV TXDWUR GLDV HP YLUWXGH GD VtQWHVH ³GH QRYR´

da vDPLODVHHDPHQRUDRVRLWRGLDVDSyVDJHUPLQDomR possivelmente devido ao esgotamento das reservas de amido QRHQGRVSHUPDGDVVHPHQWHV)LJXUD

2HVWUHVVHVDOLQRUHWDUGDDVtQWHVHHDDWLYLGDGHHQ]LPiWLFD da vDPLODVHRFRUUHQGRVtQWHVH³GHQRYR´GHVVDHQ]LPDDR

ORQJRGDJHUPLQDomRHGRGHVHQYROYLPHQWRGDSOkQWXOD(P VHPHQWHVGHIHLMmRFRUGDDVtQWHVHGDDDPLODVHpUHWDUGDGD SHOD VDOLQLGDGH TXDQGR FRPSDUDGD j WHVWHPXQKD 1HWR HW

al.

$ VDOLQLGDGH DIHWRX QHJDWLYDPHQWH H GH PDQHLUD VLJQL¿FDWLYD3”DGHJUDGDomRGRDPLGRGDVVHPHQWHVGH WULJRQDVGXDVFXOWLYDUHV)LJXUD&RPSDUDQGRDDWLYLGDGH GD ĮDPLODVH GHVFULWD DQWHULRUPHQWH )LJXUD FRP R WHRU GHDPLGR)LJXUDjPHGLGDTXHDXPHQWRXDVDOLQLGDGHR WHRUGHDPLGRDXPHQWRXGHYLGRjGLPLQXLomRGDDWLYLGDGH GHVVDHQ]LPD2VWHRUHVGHDPLGRIRUDPUHGX]LGRVDRORQJR GR WHPSR ]HUR TXDWUR H RLWR GLDV VHQGR GHJUDGDGRV HP IXQomR GD DFHOHUDomR PHWDEyOLFD H GD IRUPDomR GH QRYDV HVWUXWXUDVGDSOkQWXOD

2V WHRUHV GH FDUERLGUDWRV VRO~YHLV WRWDLV IRUDP VLJQL¿FDWLYDPHQWH 3” PDLRUHV QD FXOWLYDU GH WULJR A

Ŷ y = -1,9944x

+ 15,6x + 24,39 ( R

= 1)

Ÿ y = -1,6956x

+ 13,29x + 18,55 (R

= 1)

× y = -1,4541x

+ 11,281x + 15,39 (R

= 1) Ƈ y = -1,835x

+ 14,365x + 20,23 (R

= 1)

+ y = -1,1922x

+ 9,2437x + 13,65 (R

= 1)

0 15 30 45 60

0 4 8

B

Ŷ y = -1,8791x

+ 14,474x + 27,05 ( R

= 1) Ƈ y = -1,9025x

+ 14,715x + 23,6 (R

= 1) Ÿ y = -1,8719x

+ 14,235x + 21,25 (R

= 1)

× y = -1,6391x

+ 12,524x + 18,29 (R

= 1) + y = -1,6031x

+ 12,495x + 15,22 (R

= 1)

0 15 30 45 60

0 4 8

$WLYLGDGHGDĮDPLODVHPJGHDPLGRKLGUROLVDGRJVHPHQWH PLQ

'LDVDSyVJHUPLQDomR

FIGURA 1. Atividade de v-amilase nas cultivares de trigo BRS 177 (A) e BRS 179 (B), em função do aumento da concentração salina, sendo ]HURŶƇŸîHP0GH NaCl) determinadas aos zero, quatro e oito dias após a germinação.

A

+ y = -0,6153x

+ 2,0612x + 27,47 (R

= 1)

× y = -0,4306x

+ 1,0475x + 23,27 (R

= 1)

Ÿy = -0,4772x

+ 1,7087x + 20,46 (R

= 1) Ƈ y = -0,3825x

+ 1,25x + 17,46 (R

= 1) Ŷ y = -0,2809x

+ 0,6187x + 15,24 (R

= 1)

0 10 20 30 40

0 4 8

B ^Ŷ y = -0,3553x

+ 0,8512x + 18,66 (R

= 1) Ƈ y = -0,3844x

+ 0,885x + 20,5 (R

= 1) Ÿy = -0,5112x

+ 1,635x + 23,23 (R

= 1)

× y = -0,5747x

+ 1,7862x + 26,58 (R

= 1) + y = -0,6669x

+ 2,21x + 29,58 (R

= 1)

0 10 20 30 40

0 4 8

Teor de amido (mg de amido . g-1 semente)

Dias após germinação

FIGURA 2. Teores de amido nas cultivares BRS 177

(A) e BRS 179 (B), em função do aumento

GD FRQFHQWUDomR VDOLQD VHQGR ]HUR Ŷ

Ƈ Ÿ î H P0 GH 1D&O

determinadas aos zero, quatro e oito dias

após a germinação.

%56GRTXHQD%56)LJXUDHPWRGRVRVWHPSRV GH GHWHUPLQDomR KDYHQGR XPD UHODomR OLQHDU FRP DOWRV FRH¿FLHQWHV5

•3RUpPDPERVRVJHQyWLSRVWLYHUDP GHFUpVFLPRV QHVVHV WHRUHV FRQIRUPH R LQFUHPHQWR QDV FRQFHQWUDo}HVVDOLQDV(QWUHWDQWRQRVQtYHLVGHHP0 GH1D&OQmRIRUDPGHWHFWDGDVGLIHUHQoDVVLJQL¿FDWLYDVQRV WHRUHVGHFDUERLGUDWRVVRO~YHLVWRWDLVQRWHPSR]HURSDUDR JHQyWLSR%56HDRVRLWR'$*QR%56$VDOWHUDo}HV dos níveis de carboidratos analisados podem ser resultado GH XP PHFDQLVPR GD SODQWD SDUD UHJXODU D SHUGD GH iJXD HP UHVSRVWD DR DF~PXOR GH VDLV QR FLWRSODVPD *UHHQZD\

0XQQV DXPHQWDQGR GHVVH PRGR D DWLYLGDGH GH HQ]LPDV GH OLVH R TXH SRGH SURYRFDU PRGL¿FDo}HV QR SURFHVVRUHVSLUDWyULR

(PFXOWLYDUHVGHIHLMmRHGHEDWDWDIRUDPHQFRQWUDGRV UHVXOWDGRV VLPLODUHV VXJHULQGR TXH D ELRVVtQWHVH GH

FDUERLGUDWRV RX VXD WUDQVORFDomR QD SODQWD SRGHP VHU LQLELGDVSHODDomRGRVDO:LJQDUDMDKHWDO6DVLODND 3UDVDG

Tecidos injuriados por sal mostram aumentos FRQVLGHUiYHLV QD KLGUyOLVH GH SURWHtQDV 0HODQGHU +RUYDWK RX SRU IHULPHQWR HP WXEpUFXORV GH EDWDWD LQFUHPHQWDP D DWLYLGDGH GD 51$DVH H D WD[D UHVSLUDWyULD

%RUFKHUWDOpPGHSURPRYHUPRGL¿FDo}HVQRVQtYHLV GH FDUERLGUDWRV RV TXDLV VH VRPDGRV DOWHUDP R SURFHVVR QRUPDOGHFUHVFLPHQWRHGHVHQYROYLPHQWR

2LQFUHPHQWRQDFRQFHQWUDomRGH1D&ODIHWRXRVWHRUHV GH Do~FDUHV UHGXWRUHV QDV FXOWLYDUHV GH WULJR )LJXUD +RXYH WDPEpP XPD UHODomR OLQHDU HQWUH R GHFUpVFLPR QRV WHRUHV GH Do~FDUHV TXDQGR FRPSDUDGR FRP RV GLDV DSyV D JHUPLQDomR FRP DOWRV FRH¿FLHQWHV 5

• 3ODQWDV GH mandioca crescidas em concentrações maiores ou iguais a 75 A

Ƈ y = 0,1863x + 1,195 (R

= 0,94) Ŷ y = 0,1538x + 0,9717 (R

= 0,95)

Ÿ y = 0,1525x + 0,94 (R

= 0,94)

× y = 0,1238x + 0,825 (R

= 0,95) + y = 0,1113x + 0,7517 (R

= 0,95)

0 1 2 3

0 4 8

B

Ŷ y = 0,225x + 1,3467 (R

= 0,93) Ƈ y = 0,1725x + 1,07 (R

= 0,94) Ÿ y = 0,1588x + 1,0417 (R

= 0,94)

× y = 0,1438x + 0,935 (R

= 0,95) + y = 0,135x + 0,84 (R

= 0,952)

0 1 2 3

0 4 8

Teor de carboidratos solúveis totais (mg de glicose . g

semente)

Dias após germinação

FIGURA 3. Teores de carboidratos solúveis totais nas cultivares BRS 177 (A) e BRS 179 (B), em função do aumento da concentração salina, VHQGR]HURŶƇŸîH mM de NaCl) determinadas aos zero, quatro e oito dias após a germinação.

),*85$ 7HRUHVGHDo~FDUHVUHGXWRUHVQDVFXOWLYDUHV BRS 177 (A) e BRS 179 (B), em função do aumento da concentração salina, sendo ]HURŶƇŸîHP0GH NaCl) determinadas aos zero, quatro e oito dias após a germinação.

A Ŷ y = 0,0388x + 0,2417 (R

= 0,94) Ƈ y = 0,0338x + 0,205 (R

= 0,91)

Ÿ y = 0,03x + 0,1867 (R

= 0,95)

× y = 0,025x + 0,1633 (R

= 0,95) + y = 0,0225x + 0,1533 (R

= 0,94)

0 0,2 0,4 0,6

0 4 8

B Ŷ y = 0,0413x + 0,2617 (R

= 0,95) Ƈ y = 0,0375x + 0,2133 (R

= 0,93)

Ÿ y = 0,0313x + 0,2017 (R

= 0,97)

× y = 0,025x + 0,1867 (R

= 0,92) + y = 0,025x + 0,1633 (R

= 0,95)

0 0,2 0,4 0,6

0 4 8

Teor de açúcares redutores (mg de glicose . g

semente)

Dias após germinação

A _Ŷ y = -1,0925x

+ 8,9675x + 2,09 (R

= 1)

× y = -0,5825x

+ 4,9x + 1,27 (R

= 1) Ÿ y = -1,0366x

+ 8,4662x + 1,85 (R

= 1)

+ y = -0,4553x

+ 3,8913x + 1,19 (R

= 1) Ƈ y = -1,0303x

+ 8,4512x + 1,89 (R

= 1)

0 10 20 30

0 4 8

B ^Ŷ y = -1,2403x

+ 10,444x + 2,65 (R

= 1) Ƈ y = -1,0103x

+ 8,5137x + 2,1 (R

= 1) Ÿy = -1,0122x

+ 8,4887x + 2,07 (R

= 1)

+ y = -0,5125x

+ 4,355x + 1,26 (R

= 1)

× y = -0,6506x

+ 5,4675x + 1,39 (R

= 1)

0 10 20 30

0 4 8

Teor de proteínas (mg de proteína . g

semente)

Dias após germinação

),*85$ 7HRUHVGHSURWHtQDVQDVFXOWLYDUHV%56 (A) e BRS 179 (B), em função do aumento GD FRQFHQWUDomR VDOLQD VHQGR ]HUR Ŷ Ƈ Ÿ î H P0 GH 1D&O determinadas aos zero, quatro e oito dias após a germinação.

P0GH1D&OGXUDQWHRSHUtRGRH[SHULPHQWDOGLPLQXHPRV WHRUHVGHDo~FDUHVUHGXWRUHV/LPDHWDO

+RXYHUHGXomRVLJQL¿FDWLYD3”QRWHRUSURWpLFR GDV VHPHQWHV GH WULJR GDV FYV %56 H %56 FRP LQFUHPHQWR QD FRQFHQWUDomR VDOLQD )LJXUD 1DV GXDV FXOWLYDUHV QR HQWDQWR RFRUUHX JUDQGH DXPHQWR QR WHRU GH SURWHtQDV DRV TXDWUR GLDV HP YLUWXGH GD VtQWHVH ³GH QRYR´

GH ĮDPLODVH )LJXUD 51

H SURWHDVHV GLPLQXLQGR HVVHWHRUDRVRLWR'$*GHYLGRDRHVJRWDPHQWRQRVWHRUHV GH DPLGR )LJXUD SRUpP FRP YDORUHV DLQGD PDLRUHV GR TXH D TXDQWLGDGH SURWpLFD REWLGD DRV ]HUR GLDV DSyV D JHUPLQDomR $ FDSDFLGDGH GH R HPEULmR VLQWHWL]DU QRYDV SURWHtQDVGXUDQWHDJHUPLQDomRpGLPLQXtGDTXDQGRRQtYHO GHKLGUDWDomRGLPLQXLRVWHFLGRVQmRFUHVFHPHRPRGHORGH

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CONCLUSÕES

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