AACL Bio flux , 2 01 3 , Vo lum e 6 , Issue 2 .
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AACL BI OFLUX
Aqu a cu lt u re , Aqu a riu m , Con se rv a t ion & Le gisla t ion
I n t e rn a t ion a l Jou rn a l of t h e Bioflu x Soci e t y
M ix e d st o ck a n a lysis ( M SA) - a t ool for p rope r
m a n a ge m e n t in f ish e rie s
Vior ica Co
ş
ier
1, I . Valent in Pet r escu- Mag
1,2,31Universit y of Agricult ure Science and Vet erinary Medicine of Cluj -Napoca, Cluj , Rom ania; 2I on I onescu de la Brad Universit y of Agricult ural Sciences and Vet erinary Medicine I ași,
POSDRU Proj ect ( European Social Fund) : Post doctoral School in Agricult ure and Vet erinary Medicine Area), Rom ania; 3SC Bioflux SRL, Cluj -Napoca, Romania.
Corresponding author: V Coşier, [email protected]
Abst ract. For nat ural environm ent , it is crucial t o develop a fishery m anagem ent plan, out lining conservation and rest oration m easures. From this point of view , if t he fishery is m anaged as a single unit (st ock) , there is a huge pot ent ial of overfishing of t he less abundant population. I n order t o achieve populat ion levels t hat support harvest s or t o prot ect a particular vulnerable population from ant hropogenic pressure, t he uses of genet ic m arkers inform at ion have been proposed in fishery m anagem ent . Mixed st ock analysis uses genet ic m arkers inform ation in several source populat ions and in a single m ixt ure population t o est im ate proport ional cont ribution of each source t o t he m ixed population.
Key W ords: m ixed st ock analysis, MSA, genetic m arkers, fisheries.
Su m m a ry. Rare, endangered or econom ically im port ant fish species reproduct ion,
st ocking and conservat ion are nowadays new challenges for t he cut t ing edge t echnologies and laboratories. Som e of t hese species have very part icular reproduct ion biology ( Arbuat t i et al 2011; Geru et al 2012) and require specific rearing and stocking condit ions.
Unt il now, t he genet ic dat a have proven ext rem ely useful in ident ifying st ock groups in com plex m ixt ures. Genet ic m ixed- st ock analysis used genet ic dat a t o est im ate t he m ixt ure proport ion in a sam ple of t he m ixt ure, or t o assign t he individuals in t he sam ple t o baseline populat ion. These m et hods allow t he est imat ion of t he relat ive exploit ation, m ort alit y and harvest rat es of t he different com ponent s of m ixed- st ock fisheries ( Vidar et al 2008) . First, t he genet ic variat ions at t he prot ein level and of enzym es were applied ( allozym es) in various st udies, t hen m icrosat ellit e loci, m t DNA polym orphism s and m ore recent ly, t he most abundant variat ion on DNA level – t he SNP polym orphism s.
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expect ed het erozygousy, t he average FST value across neut ral and non- neut ral loci and t he global pair- wise FST are det erm ined in order t o establish genetic diversit y in eit her real or sim ulat ed analysis. For t he assignment and MSA of sam ples, different program m es were developed ( GeneClass, Bayes, ONCOR) ( see details in Vidar et al 2008) .
Re fe re n ce s
Arbuat t i A. , Trent ini R. , Bernabò N., Lucidi P. , 2011 Cont ribut ion t o t he biology of t he endangered Mexican fish, Zoogonet icus t equila, and suggest ions for it s indoor m anagem ent . AACL Bioflux 4( 5) : 670- 683.
Geru L., Trofim ov A. , Ruxanda F., Rus V. , Radu I . , Pop R., Miclăuş V. , 2012 Ovarian m orphology of pike (Esox lucius) from t he Danube River, during t he oogenesis cycle. Annals of t he Romanian Societ y for Cell Biology 17( 1) : 307- 311.
Pella J. J. , Milner G. B., 1987 Use of genet ic marks in st ock com posit ion analysis. Pages 274- 276 in N. Rym an, and F. Ut t er ( eds) . Populat ion genet ics and fisheries m anagem ent . Universit y of Washingt on Press, Seat t le, WA.
Shaklee J. B., Beacham T. D., Seeb L. , White B. A., 1999 Managing fisheries using genet ic dat a: case st udies from four species of Pacific salm on. Fisheries Research 43( 1- 3) : 45- 78.
Vidar W., Knut E. J., Geir D. , Svein E. F., 2008 Mixed st ock analysis and t he power of different classes of m olecular m arkers in discrim inat ing coast al and oceanic At lant ic cod (Gadus m orhua L. ) on t he Lofoten spawning grounds, Nort hern Norway. Hydrobiologia 606: 7–25. DOI 10. 1007/ s10 750- 008- 934 9- 5
Received: 25 Oct ober 2012. Accept ed: 12 Novem ber 2012. Published online: 26 Novem ber 2012. Authors:
Viorica Coşier, Universit y of Agriculture Science and Vet erinary Medicine of Cluj- Napoca, 3-5 Mănăştur Street, Cluj- Napoca, Cluj , Rom ania, European Union, e-m ail: viorica.cosier@gm ail.com
I oan Valentin Pet rescu- Mag, I on I onescu de la Brad University of Agricultural Sciences and Vet erinary Medicine I ași ( POSDRU Proj ect - European Social Fund - Post doct oral School in Agricult ure and Vet erinary Medicine Area) , Mihail Sadoveanu Alley 8, 700489 I ași, Romania, European Union, e-m ail: zoobiom [email protected]
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