Dec 6, 2020 06:49 am GMT
PDB ID : 6I7O EMDB ID : EMD-4427
Title : The structure of a di-ribosome (disome) as a unit for RQC and NGD quality control pathways recognition.
Authors : Tesina, P.; Cheng, J.; Becker, T.; Beckmann, R. Deposited on : 2018-11-16
Resolution : 5.30 Å(reported)
This is a wwPDB EM Validation Summary Report for a publicly released PDB entry. We welcome your comments at validation@mail.wwpdb.org
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https://www.wwpdb.org/validation/2017/EMValidationReportHelp
with speci c help available everywhere you see the Oi symbol.
The following versions of software and data (seereferencesOi) were used in the production of this report: EMDB validation analysis : 0.0.0.dev61
MolProbity : 4.02b-467
Percentile statistics : 20191225.v01 (using entries in the PDB archive December 25th 2019) Ideal geometry (proteins) : Engh & Huber (2001)
Ideal geometry (DNA, RNA) : Parkinson et al. (1996) Validation Pipeline (wwPDB-VP) : 2.15.1
1 Overall quality at a glance
O
iThe following experimental techniques were used to determine the structure: ELECTRON MICROSCOPY
The reported resolution of this entry is 5.30 Å.
Percentile scores (ranging between 0-100) for global validation metrics of the entry are shown in the following graphic. The table shows the number of entries on which the scores are based.
Metric Whole archive(#Entries) EM structures(#Entries)
Ramachandran outliers 154571 4023
Sidechain outliers 154315 3826
RNA backbone 4643 859
The table below summarises the geometric issues observed across the polymeric chains and their t to the map. The red, orange, yellow and green segments of the bar indicate the fraction of residues that contain outliers for >=3, 2, 1 and 0 types of geometric quality criteria respectively. A grey segment represents the fraction of residues that are not modelled. The numeric value for each fraction is indicated below the corresponding segment, with a dot representing fractions <=5% The upper red bar (where present) indicates the fraction of residues that have poor t to the EM map (all-atom inclusion < 40%). The numeric value is given above the bar.
Mol Chain Length Quality of chain
1 BQ 3396 1 YQ 3396 2 BR 121 2 YR 121 3 BS 157 3 YS 157 4 AW 252 4 XW 252
Continued from previous page...
Mol Chain Length Quality of chain
5 BA 386 5 YA 386 6 BE 361 6 YE 361 7 BI 294 7 YI 294 8 BM 176 8 YM 176 9 BO 223 9 YO 223 10 AA 231 10 XA 231 11 AD 190 11 XD 190 12 BD 221 12 YD 221 13 AG 169 13 XG 169 14 AJ 194 14 XJ 194 15 AM 137 15 XM 137 16 AQ 203 16 XQ 203 17 AU 197
Continued from previous page...
Mol Chain Length Quality of chain
17 XU 197 18 2 1800 18 2b 1800 19 P 206 19 Pb 206 20 Q 216 20 Qb 216 21 R 217 21 Rb 217 22 A 223 22 Ab 223 23 S 260 23 Sb 260 24 B 206 24 Bb 206 25 T 218 25 Tb 218 26 U 185 26 Ub 185 27 V 200 27 Vb 200 28 W 185 28 Wb 185 29 C 92 29 Cb 92
Continued from previous page...
Mol Chain Length Quality of chain
30 X 146 30 Xb 146 31 D 124 31 Db 124 32 Y 150 32 Yb 150 33 Z 128 33 Zb 128 34 E 119 34 Eb 119 35 F 141 35 Fb 141 36 G 125 36 Gb 125 37 H 145 37 Hb 145 38 I 143 38 Ib 143 39 J 101 39 Jb 101 40 a 87 40 ab 87 41 b 129 41 bb 129 42 c 144
Continued from previous page...
Mol Chain Length Quality of chain
42 cb 144 43 d 134 43 db 134 44 K 69 44 Kb 69 45 e 97 45 eb 97 46 f 81 46 fb 81 47 L 63 47 Lb 63 48 M 53 48 Mb 53 49 g 60 49 gb 60 50 N 73 50 Nb 73 51 O 313 51 Ob 313 52 AX 184 52 XX 184 53 BB 185 53 YB 185 54 BF 188 54 YF 188
Continued from previous page...
Mol Chain Length Quality of chain
55 BH 172 55 YH 172 56 BJ 159 56 YJ 159 57 BL 98 57 YL 98 58 AB 134 58 XB 134 59 AE 135 59 XE 135 60 AH 120 60 XH 120 61 AK 124 61 XK 124 62 AN 135 62 XN 135 63 AR 148 63 XR 148 64 AV 58 64 XV 58 65 AY 100 65 XY 100 66 BC 109 66 YC 109 67 BG 127
Continued from previous page...
Mol Chain Length Quality of chain
67 YG 127 68 BK 106 68 YK 106 69 BN 112 69 YN 112 70 BP 119 70 YP 119 71 AC 99 71 XC 99 72 AF 82 72 XF 82 73 AI 77 73 XI 77 74 AL 50 74 XL 50 75 AO 52 75 XO 52 76 AS 25 76 XS 25 77 AP 105 77 XP 105 78 AT 91 78 XT 91 79 BU 312 79 YU 312
Continued from previous page...
Mol Chain Length Quality of chain
80 n 76
81 m 75
82 nb 76
83 mb 77
2 Entry composition
O
iThere are 85 unique types of molecules in this entry. The entry contains 405889 atoms, of which 0 are hydrogens and 0 are deuteriums.
In the tables below, the AltConf column contains the number of residues with at least one atom in alternate conformation and the Trace column contains the number of residues modelled with at most 2 atoms.
Molecule 1 is a RNA chain called 25S ribosomal RNA.
Mol Chain Residues Atoms AltConf Trace
1 BQ 3127 66891 29878 12066 21820 3127Total C N O P 0 0
1 YQ 3127 66891 29878 12066 21820 3127Total C N O P 0 0
Molecule 2 is a RNA chain called 5S ribosomal RNA.
Mol Chain Residues Atoms AltConf Trace
2 BR 121 Total2579 1152 461 845 121C N O P 0 0
2 YR 121 Total2579 1152 461 845 121C N O P 0 0
Molecule 3 is a RNA chain called 5.8S ribosomal RNA.
Mol Chain Residues Atoms AltConf Trace
3 BS 157 Total3333 1491 584 1101 157C N O P 0 0
3 YS 157 Total3333 1491 584 1101 157C N O P 0 0
Molecule 4 is a protein called 60S ribosomal protein L2-A.
Mol Chain Residues Atoms AltConf Trace
4 AW 252 Total1912 1190 388 333 1C N O S 0 0
4 XW 252 Total1912 1190 388 333 1C N O S 0 0
Mol Chain Residues Atoms AltConf Trace
5 BA 386 Total3075 1950 584 533 8C N O S 0 0
5 YA 386 Total3075 1950 584 533 8C N O S 0 0
Molecule 6 is a protein called 60S ribosomal protein L4-A.
Mol Chain Residues Atoms AltConf Trace
6 BE 361 Total2748 1729 522 494 3C N O S 0 0
6 YE 361 Total2748 1729 522 494 3C N O S 0 0
Molecule 7 is a protein called 60S ribosomal protein L5.
Mol Chain Residues Atoms AltConf Trace
7 BI 294 Total2359 1489 412 456 2C N O S 0 0
7 YI 294 Total2359 1489 412 456 2C N O S 0 0
Molecule 8 is a protein called 60S ribosomal protein L6-A.
Mol Chain Residues Atoms AltConf Trace
8 BM 157 Total1248 806 224 217 1C N O S 0 0
8 YM 157 Total1248 806 224 217 1C N O S 0 0
Molecule 9 is a protein called 60S ribosomal protein L7-A.
Mol Chain Residues Atoms AltConf Trace
9 BO 223 Total1791 1155 325 310 1C N O S 0 0
9 YO 223 Total1791 1155 325 310 1C N O S 0 0
Molecule 10 is a protein called 60S ribosomal protein L8-A.
Mol Chain Residues Atoms AltConf Trace
10 AA 231 Total1763 1130 316 314 3C N O S 0 0
Continued from previous page...
Mol Chain Residues Atoms AltConf Trace
10 XA 231 Total1763 1130 316 314 3C N O S 0 0
Molecule 11 is a protein called 60S ribosomal protein L9-A.
Mol Chain Residues Atoms AltConf Trace
11 AD 190 Total1510 957 273 276 4C N O S 0 0
11 XD 190 Total1510 957 273 276 4C N O S 0 0
Molecule 12 is a protein called 60S ribosomal protein L10.
Mol Chain Residues Atoms AltConf Trace
12 BD 209 Total1696 1077 321 293 5C N O S 0 0
12 YD 209 Total1696 1077 321 293 5C N O S 0 0
Molecule 13 is a protein called 60S ribosomal protein L11-B.
Mol Chain Residues Atoms AltConf Trace
13 AG 169 Total1353 847 253 249 4C N O S 0 0
13 XG 169 Total1353 847 253 249 4C N O S 0 0
Molecule 14 is a protein called 60S ribosomal protein L13-A.
Mol Chain Residues Atoms AltConf Trace
14 AJ 194 Total1548 965 316 267C N O 0 0
14 XJ 194 Total1548 965 316 267C N O 0 0
Molecule 15 is a protein called 60S ribosomal protein L14-A.
Mol Chain Residues Atoms AltConf Trace
15 AM 137 Total1059 678 200 179 2C N O S 0 0
Molecule 16 is a protein called 60S ribosomal protein L15-A.
Mol Chain Residues Atoms AltConf Trace
16 AQ 203 Total1720 1077 361 281 1C N O S 0 0
16 XQ 203 Total1720 1077 361 281 1C N O S 0 0
Molecule 17 is a protein called 60S ribosomal protein L16-A.
Mol Chain Residues Atoms AltConf Trace
17 AU 197 Total1555 1003 289 262 1C N O S 0 0
17 XU 197 Total1555 1003 289 262 1C N O S 0 0
Molecule 18 is a RNA chain called 18S ribosomal RNA.
Mol Chain Residues Atoms AltConf Trace
18 2 1758 37455 16745 6624 12328 1758Total C N O P 0 0
18 2b 1758 37455 16745 6624 12328 1758Total C N O P 0 0
Molecule 19 is a protein called 40S ribosomal protein S0-A.
Mol Chain Residues Atoms AltConf Trace
19 P 206 Total1583 1017 281 283 2C N O S 0 0
19 Pb 206 Total1583 1017 281 283 2C N O S 0 0
Molecule 20 is a protein called 40S ribosomal protein S1-A.
Mol Chain Residues Atoms AltConf Trace
20 Q 216 Total1722 1091 312 315 4C N O S 0 0
20 Qb 216 Total1722 1091 312 315 4C N O S 0 0
Mol Chain Residues Atoms AltConf Trace
21 R 217 Total1635 1047 289 297 2C N O S 0 0
21 Rb 217 Total1635 1047 289 297 2C N O S 0 0
Molecule 22 is a protein called 40S ribosomal protein S3.
Mol Chain Residues Atoms AltConf Trace
22 A 223 Total1734 1101 313 314 6C N O S 0 0
22 Ab 223 Total1734 1101 313 314 6C N O S 0 0
Molecule 23 is a protein called 40S ribosomal protein S4-A.
Mol Chain Residues Atoms AltConf Trace
23 S 260 Total2068 1316 389 360 3C N O S 0 0
23 Sb 260 Total2068 1316 389 360 3C N O S 0 0
Molecule 24 is a protein called 40S ribosomal protein S5.
Mol Chain Residues Atoms AltConf Trace
24 B 206 Total1609 1007 300 299 3C N O S 0 0
24 Bb 206 Total1609 1007 300 299 3C N O S 0 0
Molecule 25 is a protein called 40S ribosomal protein S6-A.
Mol Chain Residues Atoms AltConf Trace
25 T 218 Total1755 1102 337 313 3C N O S 0 0
25 Tb 218 Total1755 1102 337 313 3C N O S 0 0
Molecule 26 is a protein called 40S ribosomal protein S7-A.
Mol Chain Residues Atoms AltConf Trace
26 U 185 Total1486 954 266 266C N O 0 0
Continued from previous page...
Mol Chain Residues Atoms AltConf Trace
26 Ub 185 Total1486 954 266 266C N O 0 0
Molecule 27 is a protein called 40S ribosomal protein S8-A.
Mol Chain Residues Atoms AltConf Trace
27 V 188 Total1489 925 298 264 2C N O S 0 0
27 Vb 188 Total1489 925 298 264 2C N O S 0 0
Molecule 28 is a protein called 40S ribosomal protein S9-A.
Mol Chain Residues Atoms AltConf Trace
28 W 185 Total1494 943 289 261 1C N O S 0 0
28 Wb 185 Total1494 943 289 261 1C N O S 0 0
Molecule 29 is a protein called 40S ribosomal protein S10-A.
Mol Chain Residues Atoms AltConf Trace
29 C 92 Total741 478 121 140 2C N O S 0 0
29 Cb 92 Total741 478 121 140 2C N O S 0 0
Molecule 30 is a protein called 40S ribosomal protein S11-A.
Mol Chain Residues Atoms AltConf Trace
30 X 146 Total1168 747 221 197 3C N O S 0 0
30 Xb 146 Total1168 747 221 197 3C N O S 0 0
Molecule 31 is a protein called 40S ribosomal protein S12.
Mol Chain Residues Atoms AltConf Trace
31 D 124 Total890 560 156 172 2C N O S 0 0
Molecule 32 is a protein called 40S ribosomal protein S13.
Mol Chain Residues Atoms AltConf Trace
32 Y 150 Total1192 759 224 207 2C N O S 0 0
32 Yb 150 Total1192 759 224 207 2C N O S 0 0
Molecule 33 is a protein called 40S ribosomal protein S14-B.
Mol Chain Residues Atoms AltConf Trace
33 Z 128 Total949 582 188 176 3C N O S 0 0
33 Zb 128 Total949 582 188 176 3C N O S 0 0
Molecule 34 is a protein called 40S ribosomal protein S15.
Mol Chain Residues Atoms AltConf Trace
34 E 119 Total939 595 176 161 7C N O S 0 0
34 Eb 119 Total939 595 176 161 7C N O S 0 0
Molecule 35 is a protein called 40S ribosomal protein S16-A.
Mol Chain Residues Atoms AltConf Trace
35 F 141 Total1105 708 203 194C N O 0 0
35 Fb 141 Total1105 708 203 194C N O 0 0
Molecule 36 is a protein called 40S ribosomal protein S17-A.
Mol Chain Residues Atoms AltConf Trace
36 G 125 Total1001 625 188 186 2C N O S 0 0
36 Gb 125 Total1001 625 188 186 2C N O S 0 0
Mol Chain Residues Atoms AltConf Trace
37 H 145 Total1192 743 237 210 2C N O S 0 0
37 Hb 145 Total1192 743 237 210 2C N O S 0 0
Molecule 38 is a protein called 40S ribosomal protein S19-A.
Mol Chain Residues Atoms AltConf Trace
38 I 143 Total1112 694 208 208 2C N O S 0 0
38 Ib 143 Total1112 694 208 208 2C N O S 0 0
Molecule 39 is a protein called 40S ribosomal protein S20.
Mol Chain Residues Atoms AltConf Trace
39 J 101 Total805 512 145 147 1C N O S 0 0
39 Jb 101 Total805 512 145 147 1C N O S 0 0
Molecule 40 is a protein called 40S ribosomal protein S21-A.
Mol Chain Residues Atoms AltConf Trace
40 a 87 Total684 420 125 137 2C N O S 0 0
40 ab 87 Total684 420 125 137 2C N O S 0 0
Molecule 41 is a protein called 40S ribosomal protein S22-A.
Mol Chain Residues Atoms AltConf Trace
41 b 129 Total1021 650 188 180 3C N O S 0 0
41 bb 129 Total1021 650 188 180 3C N O S 0 0
Molecule 42 is a protein called 40S ribosomal protein S23-A.
Mol Chain Residues Atoms AltConf Trace
42 c 144 Total1121 708 220 191 2C N O S 0 0
Continued from previous page...
Mol Chain Residues Atoms AltConf Trace
42 cb 144 Total1121 708 220 191 2C N O S 0 0
Molecule 43 is a protein called 40S ribosomal protein S24-A.
Mol Chain Residues Atoms AltConf Trace
43 d 134 Total1073 676 208 189C N O 0 0
43 db 134 Total1073 676 208 189C N O 0 0
Molecule 44 is a protein called 40S ribosomal protein S25-A.
Mol Chain Residues Atoms AltConf Trace
44 K 69 Total558 357 103 98C N O 0 0
44 Kb 69 Total558 357 103 98C N O 0 0
Molecule 45 is a protein called 40S ribosomal protein S26-B.
Mol Chain Residues Atoms AltConf Trace
45 e 97 Total769 475 160 129 5C N O S 0 0
45 eb 97 Total769 475 160 129 5C N O S 0 0
Molecule 46 is a protein called 40S ribosomal protein S27-A.
Mol Chain Residues Atoms AltConf Trace
46 f 81 Total610 382 110 113 5C N O S 0 0
46 fb 81 Total610 382 110 113 5C N O S 0 0
Molecule 47 is a protein called 40S ribosomal protein S28-A.
Mol Chain Residues Atoms AltConf Trace
47 L 63 Total497 306 99 91 1C N O S 0 0
Molecule 48 is a protein called 40S ribosomal protein S29-A.
Mol Chain Residues Atoms AltConf Trace
48 M 53 Total442 274 92 72 4C N O S 0 0
48 Mb 53 Total442 274 92 72 4C N O S 0 0
Molecule 49 is a protein called 40S ribosomal protein S30-A.
Mol Chain Residues Atoms AltConf Trace
49 g 60 Total475 299 98 77 1C N O S 0 0
49 gb 60 Total475 299 98 77 1C N O S 0 0
Molecule 50 is a protein called Ubiquitin-40S ribosomal protein S31.
Mol Chain Residues Atoms AltConf Trace
50 N 73 Total556 352 105 95 4C N O S 0 0
50 Nb 73 Total556 352 105 95 4C N O S 0 0
There are 2 discrepancies between the modelled and reference sequences:
Chain Residue Modelled Actual Comment Reference
N 97 ALA LYS con ict UNP P05759
Nb 97 ALA LYS con ict UNP P05759
Molecule 51 is a protein called Guanine nucleotide-binding protein subunit beta-like protein.
Mol Chain Residues Atoms AltConf Trace
51 O 313 Total2403 1521 411 463 8C N O S 0 0
51 Ob 313 Total2403 1521 411 463 8C N O S 0 0
There are 2 discrepancies between the modelled and reference sequences:
Chain Residue Modelled Actual Comment Reference
O 161 ALA LYS con ict UNP P38011
Molecule 52 is a protein called 60S ribosomal protein L17-A.
Mol Chain Residues Atoms AltConf Trace
52 AX 175 Total1378 856 273 249C N O 0 0
52 XX 175 Total1378 856 273 249C N O 0 0
Molecule 53 is a protein called 60S ribosomal protein L18-A.
Mol Chain Residues Atoms AltConf Trace
53 BB 185 Total1441 908 290 241 2C N O S 0 0
53 YB 185 Total1441 908 290 241 2C N O S 0 0
Molecule 54 is a protein called 60S ribosomal protein L19-A.
Mol Chain Residues Atoms AltConf Trace
54 BF 183 Total1482 911 320 251C N O 0 0
54 YF 188 Total1522 935 326 261C N O 0 0
Molecule 55 is a protein called 60S ribosomal protein L20-A.
Mol Chain Residues Atoms AltConf Trace
55 BH 172 Total1445 930 267 244 4C N O S 0 0
55 YH 172 Total1445 930 267 244 4C N O S 0 0
Molecule 56 is a protein called 60S ribosomal protein L21-A.
Mol Chain Residues Atoms AltConf Trace
56 BJ 159 Total1276 805 246 221 4C N O S 0 0
56 YJ 159 Total1276 805 246 221 4C N O S 0 0
Mol Chain Residues Atoms AltConf Trace
57 BL 98 Total778 505 127 146C N O 0 0
57 YL 98 Total778 505 127 146C N O 0 0
Molecule 58 is a protein called 60S ribosomal protein L23-A.
Mol Chain Residues Atoms AltConf Trace
58 AB 134 Total993 623 187 176 7C N O S 0 0
58 XB 134 Total993 623 187 176 7C N O S 0 0
Molecule 59 is a protein called 60S ribosomal protein L24-A.
Mol Chain Residues Atoms AltConf Trace
59 AE 135 Total1089 682 219 187 1C N O S 0 0
59 XE 135 Total1089 682 219 187 1C N O S 0 0
Molecule 60 is a protein called 60S ribosomal protein L25.
Mol Chain Residues Atoms AltConf Trace
60 AH 120 Total959 617 168 172 2C N O S 0 0
60 XH 120 Total959 617 168 172 2C N O S 0 0
Molecule 61 is a protein called 60S ribosomal protein L26-A.
Mol Chain Residues Atoms AltConf Trace
61 AK 124 Total976 614 190 172C N O 0 0
61 XK 124 Total976 614 190 172C N O 0 0
Molecule 62 is a protein called 60S ribosomal protein L27-A.
Mol Chain Residues Atoms AltConf Trace
62 AN 135 Total1092 710 202 180C N O 0 0
Continued from previous page...
Mol Chain Residues Atoms AltConf Trace
62 XN 135 Total1092 710 202 180C N O 0 0
Molecule 63 is a protein called 60S ribosomal protein L28.
Mol Chain Residues Atoms AltConf Trace
63 AR 148 Total1173 749 231 190 3C N O S 0 0
63 XR 148 Total1173 749 231 190 3C N O S 0 0
Molecule 64 is a protein called 60S ribosomal protein L29.
Mol Chain Residues Atoms AltConf Trace
64 AV 58 Total462 289 100 73C N O 0 0
64 XV 58 Total462 289 100 73C N O 0 0
Molecule 65 is a protein called 60S ribosomal protein L30.
Mol Chain Residues Atoms AltConf Trace
65 AY 100 Total767 492 128 146 1C N O S 0 0
65 XY 100 Total767 492 128 146 1C N O S 0 0
Molecule 66 is a protein called 60S ribosomal protein L31-A.
Mol Chain Residues Atoms AltConf Trace
66 BC 109 Total883 559 167 156 1C N O S 0 0
66 YC 109 Total883 559 167 156 1C N O S 0 0
Molecule 67 is a protein called 60S ribosomal protein L32.
Mol Chain Residues Atoms AltConf Trace
67 BG 127 Total1020 647 205 167 1C N O S 0 0
Molecule 68 is a protein called 60S ribosomal protein L33-A.
Mol Chain Residues Atoms AltConf Trace
68 BK 106 Total850 540 165 144 1C N O S 0 0
68 YK 106 Total850 540 165 144 1C N O S 0 0
Molecule 69 is a protein called 60S ribosomal protein L34-A.
Mol Chain Residues Atoms AltConf Trace
69 BN 112 Total880 545 179 152 4C N O S 0 0
69 YN 112 Total880 545 179 152 4C N O S 0 0
Molecule 70 is a protein called 60S ribosomal protein L35-A.
Mol Chain Residues Atoms AltConf Trace
70 BP 119 Total965 612 185 167 1C N O S 0 0
70 YP 119 Total965 612 185 167 1C N O S 0 0
Molecule 71 is a protein called 60S ribosomal protein L36-A.
Mol Chain Residues Atoms AltConf Trace
71 AC 99 Total770 481 156 131 2C N O S 0 0
71 XC 99 Total770 481 156 131 2C N O S 0 0
Molecule 72 is a protein called 60S ribosomal protein L37-A.
Mol Chain Residues Atoms AltConf Trace
72 AF 82 Total650 396 142 107 5C N O S 0 0
72 XF 82 Total650 396 142 107 5C N O S 0 0
Mol Chain Residues Atoms AltConf Trace
73 AI 77 Total608 388 114 106C N O 0 0
73 XI 77 Total608 388 114 106C N O 0 0
Molecule 74 is a protein called 60S ribosomal protein L39.
Mol Chain Residues Atoms AltConf Trace
74 AL 50 Total436 272 97 65 2C N O S 0 0
74 XL 50 Total436 272 97 65 2C N O S 0 0
Molecule 75 is a protein called Ubiquitin-60S ribosomal protein L40.
Mol Chain Residues Atoms AltConf Trace
75 AO 52 Total417 259 86 67 5C N O S 0 0
75 XO 52 Total417 259 86 67 5C N O S 0 0
Molecule 76 is a protein called 60S ribosomal protein L41-B.
Mol Chain Residues Atoms AltConf Trace
76 AS 25 Total233 142 63 27 1C N O S 0 0
76 XS 25 Total233 142 63 27 1C N O S 0 0
Molecule 77 is a protein called 60S ribosomal protein L42-A.
Mol Chain Residues Atoms AltConf Trace
77 AP 105 Total847 534 170 138 5C N O S 0 0
77 XP 105 Total847 534 170 138 5C N O S 0 0
Molecule 78 is a protein called 60S ribosomal protein L43-A.
Mol Chain Residues Atoms AltConf Trace
78 AT 91 Total694 429 138 121 6C N O S 0 0
Continued from previous page...
Mol Chain Residues Atoms AltConf Trace
78 XT 91 Total694 429 138 121 6C N O S 0 0
Molecule 79 is a protein called 60S acidic ribosomal protein P0.
Mol Chain Residues Atoms AltConf Trace
79 BU 138 Total1052 672 187 190 3C N O S 0 0
79 YU 138 Total1052 672 187 190 3C N O S 0 0
Molecule 80 is a RNA chain called P-site tRNA.
Mol Chain Residues Atoms AltConf Trace
80 n 76 Total1621 723 291 531 76C N O P 0 0
There are 3 discrepancies between the modelled and reference sequences:
Chain Residue Modelled Actual Comment Reference
n 74 C - insertion GB 1329886529
n 75 C - insertion GB 1329886529
n 76 A - insertion GB 1329886529
Molecule 81 is a RNA chain called E-site tRNA.
Mol Chain Residues Atoms AltConf Trace
81 m 75 Total1589 710 279 525 75C N O P 0 0
There is a discrepancy between the modelled and reference sequences: Chain Residue Modelled Actual Comment Reference
m 11 C U con ict GB 176418
Molecule 82 is a RNA chain called A/P hybrid tRNA.
Mol Chain Residues Atoms AltConf Trace
Molecule 83 is a RNA chain called P/E hybrid tRNA.
Mol Chain Residues Atoms AltConf Trace
83 mb 77 Total1644 732 297 538 77C N O P 0 0
Molecule 84 is a RNA chain called mRNA.
Mol Chain Residues Atoms AltConf Trace
84 l 57 Total1182 530 171 424 57C N O P 0 0
Molecule 85 is ZINC ION (three-letter code: ZN) (formula: Zn).
Mol Chain Residues Atoms AltConf
85 AP 1 Total Zn1 1 0 85 XO 1 Total Zn1 1 0 85 eb 1 Total Zn1 1 0 85 e 1 Total Zn1 1 0 85 XP 1 Total Zn1 1 0 85 AT 1 Total Zn1 1 0 85 Mb 1 Total Zn1 1 0 85 N 1 Total Zn1 1 0 85 YN 1 Total Zn1 1 0 85 XT 1 Total Zn1 1 0 85 AO 1 Total Zn1 1 0 85 XF 1 Total Zn1 1 0 85 fb 1 Total Zn1 1 0 85 f 1 Total Zn1 1 0 85 M 1 Total Zn1 1 0
3 Residue-property plots
O
iThese plots are drawn for all protein, RNA, DNA and oligosaccharide chains in the entry. The rst graphic for a chain summarises the proportions of the various outlier classes displayed in the second graphic. The second graphic shows the sequence view annotated by issues in geometry and atom inclusion in map density. Residues are color-coded according to the number of geometric quality criteria for which they contain at least one outlier: green = 0, yellow = 1, orange = 2 and red = 3 or more. A red diamond above a residue indicates a poor t to the EM map for this residue (all-atom inclusion < 40%). Stretches of 2 or more consecutive residues without any outlier are shown as a green connector. Residues present in the sample, but not in the model, are shown in grey.
• Molecule 1: 25S ribosomal RNA Chain BQ:
G U U3 A13 U14 C15 A26 G30 A40 A43 A48 A49 A60 A65 A66 C73 G74 G75 G76 A85 G92 G96 A109 G110 A115 A121 A122 U133 U134 C135 A150 A151 U152 U153 G156 A157 G161 A165 G170
G171 U182 G183 U184 A187 U190 U191 C200 G206 U210 U217 G218 A219 G220 A221 G234 U240 G241 U245 U246 C247 U248 G251 U252 A253 A254 A265 G269 G282 G283 A284 A285 U286 A295 U305 A306 C311 C315 A323 U329
A334 A338 C339 C346 C350 G368 A375 G376 A387 G390 A398 A399 G400 U401 A402 C403 G406 G420 G421 A422 C439 A440 U G G U G U U U U G U G C C C U C U G C U C C U U G
U G G G U A G G G G A A U C U C G C A U U U C A C U G G494 C503 U507 C515 A519 U520 A521 A522 A523 G535 U536 U545 C546 G547
G548 U555 U556 A557 U558 A559 A569 A578 G579 C580
U581 A589 A592 C593 U594 G595 C596 G597 G600 A603 G604 G609 G610 A611 U612 A619 U620 A621 A622 C636 C637 A645 A649 C650 A656 A660 G661 A665 A666 C667 G674 A677 U681 U682 U683 A691 A705 A715 A716 U719
G725 G726 G727 A735 A736 G737 C758 A761 U762 G763 U764 C765 U766 U767 C768 U776 U777 A780 G781 A784 G785 A786 A806 A807 A808 A817 G826 A830 G831 G832 G833 A837 C849 C861 U871 U872 C873 U874 U879 U897 G907 G908 G909
A914 A915 G916 A917 C918 U919 A920 C923 U932 A936 G937 C944 C959 U960 C961 A962 G974 U979 G984 G991 A1002 G1010 U1014 U1015 C1016 C1017 G1018 G1021 G1024 A1025 A1026 A1027 U1028
G1029 U1033 U1034 G1035 U1041 A1047 A1048 C1049
A1062 G1063 A1064 A1065 G1066 U1067 G1072 A1075 U1081 U1082 A1085 C1086 G1087 A1093 U1096 G1097 A1098 A1103 G1104 G1117 A1120 G1131 A1143 G1152 A1153 A1154 C1155 A1159 A1163 G1178 A1179 A1180 U1181 A1182 G1186 C1187 C1201 A1202 U1208 A1221 G1222
A1223 C1228 C1232 U1235 G1236 G1237 C1238 C1239 A1240 U1241 G1242 G1243 A1244 A1245
G1246 C1254 C1255 G1256 C1257 U1258 G1262 A1263 G1264 U1265 G1266 U1267
G1268 U1269 A1270 A1271
C1275 U1276 C1277 A1278 G1281 G1282 C1283 C1284 G1285 A1286 G1295 A1301 G1307 A1308 U1309 G1313 C1314 U1325
U1511 U1523 A1524 G1525 G1528 G1536 G1542 U1554 U1555 C1556 A1557 A1558 A1559 G1560 G1561 C1562 C1563 U1564 G A U U U U A U G1573 C1574 A1575 G1576 G1577 C1578 C1579 A1580 C1581 C1582 A1583 A1588 A1589 C1597 G1604 A1605 U1606 U1607 U1620 U1629 C1639
A1642 A1643 C1644 U1645 G1655 G1658 U1659 C1660 G1661 G1662 A1683 U1692 C1693 U1694 U1695 U1716 U1717 G1718 U1724 G1743 A1750 G1751 A1760 C1761 C1762 U1763 U1764 U1765 G1766 G1770 G1775 G1780 G1796 A1797 G1808 A1814 U1815 A1816 G1817 U1818 U1821 A1839 U1840
A1841 A1842 C1846 A1847 G1848 C1849 A1850 G1851 A1858 G1863 C1866 G1875 G1878 A1879 U1880 A1883 A1884 A1893 G1897 C1904 G1905 G1906 A1930 G1935 G1953 G U A G U G A G G G C C U U G G U C A G A C G C A G C G G G C G U G C U U G U G G A C U G C U U G G U G G G G C U U G C U C U G C U A G G C G G A C U A C U U G C G
U G C C U U G U U G U A G A C G G C C U U G G U A G G U C U C U U G U A G A C C G U C G C U U G C U A C A A U U A A2093 A2100
C2101 U2102 G2111 U2112 A2113 C2114 G2121 G2122 A2131 C2132 U2133 A2142 A2143 A2144 A2145 A2158 G2169 U2170 A2188 G2194 C2204 U2205 G2206 A2207 A2208 U2209 G2210 U2211 C2212 A2223 A2228 A2229 C2230 C2231 A2232 A2244 C2245 G2246 G2247 C2248 G2249 G2250 G2251 A2252 G2253 U2254 A2255 A2256
C2257 U2260 G2261 A2262 C2263 U2266 U2269 G2272 G2273 G2276 A2279 A2280 A2281 G2288 C2304 G2307 C2308 A2309 U2310 A2313 U2314 G2315 G2335 U2336 C2350 C2362 G2369 A2373 C2374 G2375 G2376 U2388 G2393 A2397 A2401 A2402 G2403 A2404 C2405 U2411
G2412 A2413 G2418 A2419 U2434 G2435 U2436 G2437 A2438 A2441 G2442 A C A U A G A G G G U G U A G A A U A A G U G G G A G C U U C G G C G C C A G U G A A A U A
C C A C U A C C U U U A U A G U2504 U2505 U2506 U2510 A2511 C2512 U2513 U2514 A2515 G2522 A2523 A2524 G2525 G2528 A2529 G2530 G2534 U2537
U2538 C2539 A2540 U2541 U2542
U2543 U2544 C2545 C2552 U2553 A2554 G2555 C2566 C2567 C2568 A2569 U2570 U2571 C2572 G2573
G2574 C2583 G2584 G2585 G2586 U2587 A2593 C2594 C2600 G2606 G2607 U2612 U2613 G2614 G2619 A2626 C2627 A2628 U2629 C2644 U2652 A2656 G2662 G2663 G2672 A2673 A2674 G2677 A2678 U2683 A2689 G2690 A2691 A2694 A2695 A2696 A2704 U2713 G2714 G2720
G2728 U2729 C2737 A2740 C2741 G2753 C2772 C2773 G2778 A2779 G2796 A2799 G2800 A2801 A2802 A2803 C2810 G2814 A2817 U2818 A2819 A2820 C2821 A2845 U2846 A2847 A2853 U2860 U2861 C2870 G2871 A2872 U2873 C2876 C2885 U2886 A2887 U2888 C2889 C2894 C2899 A2900
A2910 U2923 C2928 U2935 A2936 G2937 G2938 A2941 C2942 G2943 G2947 G2957 U2965 A2971 G2972 U2978 U2979 C2983 G2990 U2996 G2997 A3012 U3023 G3030 A3049 U3058 G3059 U3068 U3078 U3079 G3080 A3086 C3092 G3101 G3109
A3113 G3116 U3121 A3122 A3123 A3130 U3131 A3142 C3143 G3144 A3150 U3151 U3152 U3153 C U U U3157 G3158 C3161 A3165 A3170 U3171 A3172 G3173 A3174 U3175 G3176 U3179 A3180 C3181 G3182 A3187 U3195 U3196 U3207 A3210 U3214 A3215 G3216 C3217 A3218 G3219 A3227 C3228
G3229 G3238 A3244 A3245 G3246 U3251 U3259 G3263 A3267 A3268 U3269 U3270 G3271 C3272 U3275 G3276 U3277 C3278 A3279 U3280 U3281 G3286 G3289 G3290 A3294 A3295 U3304 G3309 U3313 A3316 U3317 G3318 U3319 U3334 U3341 A3342 G3345 G3348 C3349 C3350 U3351 U3352
G3353 U3354 U3355 G3356 U3357 U3358 C3367 U3368 G3369 C3378 U3382 U3389 U3396
Chain YQ:
G U U3 A6 U14 C15 A16 A26 G30 C36 A39 A40 A45 U46 C47 A48 A49 A57 A60 A65 A66 C72 C73 G74 G75 G76 A77 G92 G96 A109 G110 C111 A121 A122 U133 U134 C135 G136 U149 A150 G155 G156
A157 G161 A165 G170 G171 G172 U182 G183 U184 C185 U186 A187 U188 G189 U190 U191 C200 C208 A209 U210 A211 G212 A213 U217 G218 A219 G220 A221 U240 G241 U245 U246 C247 U248 G251 U252 A253 A254 C263 G264 A265 A266 G269 G282 G283 A284 A285
U286 A295 U298 C315 A323 A327 U328 U329 G330 A334 A338 C339 U343 A344 G345 C346 G347 A348 A349 C350 A351 A352 G364 G368 A375 G376 G390 A398 U401 A402 C403 G404 U405 G406 A418 G419 G420 G421 A422 G425 C439 A440 U
G G U G U U U U G U G C C C U C U G C U C C U U G U G G G U A G G G G A A U C U C G C A U U U C A C U G G494 C503 U507 C515
A519 U520 A521 U536 C546 G547
G548 A551 U555 U556 A557 U558 A559 A569 A578 G579 A589 A592 C593 U594 G600 A603 G604 G609 G610 A611 U612 U620 A621 A622 C636 C641 U642 A645 A649 A656 G661 U662 C663 C667
A677 U681 U682 U689 A690 A691 A705 A706 U707 G708 A715 A716 U719 A720 G726 C729 A735 A736 G737 G742 C753 G760 A761 C765 U766 U767 G770 U776 U777 A780 G781 G785 A786 G787 C788 C803 A806 A807 A808 G815 A816
A817 A820 A830 G831 G832 A837 G857 C861 C873 U874 U879 A882 C886 U897 G907 G908 G912 A913 A914 A915 G916 A917 G924 A925 A926 A933 G937 C944 G953 C959 U960 C961 A962 G974 U979 A980 U981 G984
G991 G1001 A1002 G1010 U1014 U1015 C1016 C1017 G1018 G1021 G1024 A1025
A1026 A1027 U1028
G1029 U1033 U1034 G1035 C1038 A1047 A1048 C1049 A1062 G1063 A1064 A1065 U1071 G1072 A1079 A1080 U1081 U1082 A1085 A1093 U1094 U1095 U1096 G1097 A1098 A1103 G1104 A1112 G1117
C1118 C1119 A1129 A1130 G1131 A1143 U1144 G1145 C1146 G1152 A1153 A1154 C1155 A1159 C1160 A1165 G1178 A1179 A1180 U1181 A1182 C1185 G1186 C1187 C1192 A1193 G1194 A1195 C1196 C1201 A1202 G1206 G1213 U1220
A1221 G1222 A1223 G1234 U1235 G1236
G1237 C1238 C1239 A1240 U1241 G1242 G1243 A1244 A1245
G1246 U1258 G1262 A1263 G1264 U1265 G1268
U1269 A1270 A1271 A1274
C1277 A1278 C1283 C1284 G1285 A1286 A1287 G1295 A1301 U1305 G1306 G1307 A1308 U1309 G1310 C1327 C1328 U1329 A1330 U1331 A1332 C1333 G1346 U1347 U1348 G A U A1352 U1353 G1354 A1355 U1356 G1357 C1364
A1373 G1377 C1385 A1386 A1399 G1400 G1404 G1408 A1418 A1419 C1420 G1421 G1434 C1437 G1443 G1444 U1445 A1446 C1451 A1452 U1455 A1461 A1475 G1476 A1477 C1478 A1481 A1482 G1483 U1484 G1487 G1488 U1495 C1496 C1505 C1508 A1509 G1510 U1511 U1523
A1524 G1525 U1533 G1544 A1545 A1546 U1549 U1554 U1555 C1556 A1559 G1560 G1561 C1562 C1563 U1564 G A U U U U A U G1573 C1574 A1575 G1576 G1577 C1578 C1579 A1580 C1581 C1582 A1583 U1584 A1587 A1588 A1589 G1590 G1591 C1596 C1597 A1605 U1606 U1607 U1620 U1629 C1639
A1642 A1643 C1644 U1645 G1655 G1658 C1665 G1677 A1683 U1694 U1716 U1717 U1722 A1723 U1724 G1736 A1741 A1750 G1751 C1759 A1760 C1761 C1762 U1763 U1764 U1765 G1766 G1769 G1770 G1775 G1780 G1796 A1797 A1798 A1799 G1808 A1814 U1815 A1816 G1817 U1818
C U U G G U C A G A C G C A G C G G G C G U G C U U G U G G A C U G C U U G G U G G G G C U U G C U C U G C U A G G C G G A C U A C U U G C G U G C C U U G U U G U A G A C G G C C U U G G U A G G U C U C U U G U A G A C C G U C G C U U G C
U A C A A U U A A2093 C2101 U2102 G2111 U2112 A2113 C2114 C2118 G2121 G2122 A2131 U2135 A2139 A2145 C2146 A2149 A2158 A2168 G2169 U2184 A2188 U2189 U2190 U2191 C2192 U2193 G2194 C2204 U2205 G2206 A2207 A2208 U2209 G2210 U2211 C2212 A2213
A2223 C2227 A2228 A2229 A2244 C2245 G2246 G2249 G2250 G2251 A2252 G2253 U2254 A2255 A2256 C2257
U2258
A2259 U2260 G2261 A2262 C2263 U2266 C2267 U2268 U2269 A2270 A2271 G2272 G2273 U2274 A2275 G2276 A2279 A2280 A2281 C2284 G2288 C2304 G2307 C2308 A2309 U2310 A2313 U2314 G2315 C2330 C2346
U2349 C2359 A2372 A2373 C2374 G2375 U2388 C2389 A2390 G2393 G2394 A2397 A2398 A2401 A2402 G2403 A2404 C2405 C2406 C2407 U2411 G2412 A2413 G2418 A2419 C2422 U2434 G2435 U2436 G2437 A2441 G2442 A C A U A G A G G G U G U A G A
A U A A G U G G G A G C U U C G G C G C C A G U G A A A U A C C A C U A C C U U U A U A G U2504 U2505 U2510 A2511 C2512 U2513 U2514 A2515 G2522 A2523 A2524 G2525 C2526
G2530 A2535 A2536 U2537 U2538 C2539
A2540 U2541 U2542 U2543 U2544 C2545 C2548 G2549 C2552 U2553 A2554 A2562 C2566 C2567 C2568 A2569 U2570 U2571 C2572 G2573 G2574 U2578 G2579 A2580 C2583 G2584 G2585 G2589 A2593 C2594 G2602 G2606 G2607 U2612 U2613 G2614 G2615 C2616 U2629
A2636 U2652 U2655 A2656 G2662 G2663 G2672 A2673 A2674 G2677 U2683 A2689 G2690 A2694 A2695 A2696 A2703 A2704 C2708 C2709 C2710 G2714 A2727 G2728 U2729 C2737 A2740 U2752 G2753 G2754 C2755 C2756 A2762 A2769 C2772 C2773 G2777 G2778 A2779 U2782
G2796 A2799 G2800 A2801 A2802 A2803 A2804 C2810 A2811 C2812 A2813 G2814 A2817 U2818 A2819 A2820 C2821 G2828 A2845 U2846 A2847 G2848 C2849 U2860 U2861 C2867 C2870 G2871 A2872 U2873 G2874 U2875 C2876 G2877 G2878 A2887 U2888 C2889 C2894 C2899 A2900 A2910 C2913 U2916
U2923 U2924 U2935 A2936 C2942 G2943 U2944 G2945 A2946 G2947 U2954 U2955 A2956 G2957 U2965 G2966 A2967 C2970 A2971 G2972 U2978 U2979 A2982 C2983 G2990 U2996 G2997 A3011 A3012 G3030 C3039 A3048 A3049 U3058 G3059 C3067 G3074 U3078 U3079 G3080 G3085 A3086
C3092 C3093 A3094 G3102 A3103 U3104 C3110 A3114 C3115 G3116 A3122 A3130 U3131 C3132 C3133 A3134 A3142 C3143 G3144 C3145 A3150 U3151 U3152 U3153 C U U U3157 G3158 C3159 A3165 C3166 A3170 U3171 A3172 G3173 A3174 U3175 G3176 U3179 A3180 C3181 A3187 U3195 U3196 G3197 U3198
U3207 A3210 U3214 C3217 A3218 G3219 A3227 C3228 G3229 C3235 G3238 A3243 A3244 A3245 G3246 U3251 U3259 G3263 A3267 A3268 U3269 U3270 G3271 C3272 G3276 U3277 C3278 A3279 U3280 U3281 G3286 G3289 G3290 A3294 A3295 U3304 C3308 G3309 U3313 A3316
U3317 G3318 U3334 A3335 G3340 U3341 A3342 G3345 G3348 U3351
U3352 G3353 U3354 U3355 G3356 U3357 U3358 A3362 C3367 U3368 G3369 C3378 U3384 U3389 G3390 U3396 • Molecule 2: 5S ribosomal RNA
Chain BR:
G1 G7 C19 A22 U33 U38 U50 U54 A55 G65 C73 C74 G75 A76 G91 G99 A102 G112 U121 • Molecule 2: 5S ribosomal RNA
Chain YR:
G1 G7 G8 C9 C10 A11 A22 U38 A46 U54 A55 G65 C73 A76 G82 G87 C93 A102 A103 G112 C113 C116 U121 • Molecule 3: 5.8S ribosomal RNA
Chain BS:
A1 C8 U34 C35 U38 G39 A48 G49 A59 C62 G63 A80 U81 U82 C83 C84 G85 U86 G87 U90 C91 G95 A96 A97 U102 G103 A104 A105 C106 A111 U112 U113 G116 U125 A138 U156 U157 • Molecule 3: 5.8S ribosomal RNA
Chain YS:
A1 U5 C8 C11 C14 C19 U23 U34 C35 A43 G46 C47 A48 G49 A59 C62 G63 A79 A80 U81 U82 C83 C84 G85 U86 G87 C91 G95 A96 A97 A104 A105 C106 A111 U112 U113 G123 G124 U125 A138 U139 G140
G148 G152 U156 U157
• Molecule 4: 60S ribosomal protein L2-A Chain AW: G2 E109 G110 K156 G214 R247 K251 T252 Q253
• Molecule 4: 60S ribosomal protein L2-A Chain XW: G2 M204 H211 S249 Q250 K251 T252 Q253
• Molecule 5: 60S ribosomal protein L3 Chain BA:
S2 R10 F46 T81 T104 D112 R116 K241 R275 R332 L387
• Molecule 5: 60S ribosomal protein L3 Chain YA:
S2 R10 R19 K34 L47 T104 D112 D140 W233 R332 K333 R334 D386 L387
• Molecule 6: 60S ribosomal protein L4-A Chain BE: S2 A17 A28 E63 R73 A89 F90 G97 R98 I145 D177 L182 L187 L236 E265 T266 V267 A268 P301 L318 L339 K342 D362 • Molecule 6: 60S ribosomal protein L4-A
Chain YE:
S2 A89 F90 R98 I145 D177 K186 P301 L318 L339 G340 S341 K342 K343 D362
• Molecule 7: 60S ribosomal protein L5 Chain BI: Q4 K5 D6 R50 L110 E130 E136 D137 R196 R218 A249 S269 R282 Q296 Q297
• Molecule 7: 60S ribosomal protein L5 Chain YI: Q4 K5 Y79 L110 D137 R196 A228 D229 D230 K254 K270 L293 A294 G295 Q296 Q297
• Molecule 8: 60S ribosomal protein L6-A Chain BM: MET S2 Y10 S33 L34 R46 R51 G67 S88 E94
E109 LYS LEU THR LYS LYS GLU LYS LYS GLU ALA ASN LEU PHE PRO GLU GLN GLN ASN K128 F176
• Molecule 8: 60S ribosomal protein L6-A Chain YM:
MET S2 Y10 L34 E59
E109 LYS LEU THR LYS LYS GLU LYS LYS GLU ALA ASN LEU PHE PRO GLU GLN GLN ASN K128 F176
Chain BO: T22 A23 E24
R33 F229 R232 N244
• Molecule 9: 60S ribosomal protein L7-A Chain YO:
T22 R232 N244
• Molecule 10: 60S ribosomal protein L8-A Chain AA: N24 A102 A117 E118 G119 K120 L189 V190 N191 E202 A205 E206 D207 E208 A209 A210 K245 S253 D254
• Molecule 10: 60S ribosomal protein L8-A Chain XA: N24 A117 E118 L162 K245 N252 S253 D254
• Molecule 11: 60S ribosomal protein L9-A Chain AD:
M1 E189
D190
• Molecule 11: 60S ribosomal protein L9-A Chain XD: M1 T35 K36 D107 E189 D190
• Molecule 12: 60S ribosomal protein L10 Chain BD: MET A2 R21 N55
K101 MET LEU SER CYS ALA GLY ALA ASP ARG LEU GLN Q113 G132
R153 A219 Q220 A221
• Molecule 12: 60S ribosomal protein L10 Chain YD: MET A2 R3 D28 R32
L48 K101 MET LEU SER CYS ALA GLY ALA ASP ARG LEU GLN Q113 D130
R139 R153 E186 R211 A219
Q220 A221
• Molecule 13: 60S ribosomal protein L11-B Chain AG: Q6 R10 D11 R94 N95 G113 I114 K115 H152 L172 D173 K174
• Molecule 13: 60S ribosomal protein L11-B Chain XG: Q6 N7 P8 D11 L12 G42 R94 N95 G113 I120 H152 L172 D173 K174
• Molecule 14: 60S ribosomal protein L13-A Chain AJ:
A2
V22
A47 P48 A60 P61 T62 R67 G74 L77 A135 S140 A141 A151
D161 N162 G163 E164 K190 A195 • Molecule 14: 60S ribosomal protein L13-A Chain XJ:
A2
R15 A47 P48 A60 P61 T62 G74 L77 A135 S140 A151
T152 D153 A195
• Molecule 15: 60S ribosomal protein L14-A Chain AM:
There are no outlier residues recorded for this chain. • Molecule 15: 60S ribosomal protein L14-A
Chain XM: S2 R55 G61 Q62 A138
• Molecule 16: 60S ribosomal protein L15-A Chain AQ: G2 A40 A146 R147 A185 K204
• Molecule 16: 60S ribosomal protein L15-A Chain XQ:
G2 A146 R147 K204
• Molecule 17: 60S ribosomal protein L16-A Chain AU: V3 L99 R117 Y199
• Molecule 17: 60S ribosomal protein L16-A Chain XU: V3 E4 P5 R59 L99 D113 Y199
• Molecule 18: 18S ribosomal RNA Chain 2:
U1 A2 U3 C4 G7 G10 C17 C25 A26 U27 G34 A43 A46 A47 C50 G57 G63 A67 A68 G69 C70 A71 A72 U73 U74 U75 A76 U77 A78 A100 A103 A104 U111 C114 G115 U116 G127 C130 C131 U132
U133 U134
A135
C136 U137 A138 C139 A140 U141 U144 A145 U146 G153 A156 A157 U158 U159 C160 U161 C166 U178 U185 C186 G187 A188 C189 C190 C191 U192 U193 U194 G195 G196 A197 A200 A215 U216 A217 A218 A219 C224 U227
G228 U229 C230 U231 U232 C233 G234 G235 A236 C237 U238
C239 U240 U241 U242 G243 C250 U261 U262 A265 A271 U272 C275 C276 U277 U278 G279 U280 G281 G287 A288 U289 G290 A299 C308 C309 A312 U313 C314 A315 A316 C321 G322 A323 A333 G337 C338 C351 A352 A359 A360 C361 G365 U380
G388 A399 A400 A401 C402 G403 G404 C411 A416 A417 G418 G422 G423 C424 A425 G426 U439 U440 C444 A445 A446 U447 C448 U453 U454 C455 G459 A460 A468 C469 A470 A475 U476 A477 C484 A485 G486 G487 G488 U493 U494 C495 G496 G497 G498 U499 C500 U501
A619 A620 A621 A622 A623 G624 U639 U649 U650 C653 C654 G655 G656 U657 C658
C G A U U U U U U C G U G U A C U G676 G677 A678 U679 U680 U681 C682 C683 A684 A685 C686 G687 U694 U695 C696 C697 G702 G703 C704 U705 A706 A707 C708
C709 U710 U711 G712 A713 G714 U715 C716 C717 U718
U719
G720 U721 G722 G723 C724 U725 C726 U727 U728 G729 G730 C731 G732 A733 A734 C735 C736 A737 G738 C741 U742 U743 U744 U745 A755 A756 A757 U758 G765 U766 A771 A774 G778 U781 U782 G783 C784 A789 U794 U795
A806 A811 A812 G815 G816 A817 C818 G819 U820 U821 U822 G823 G824 A829 U830 U831 U832 U833 G837 G838 U839 U840 U841 G846 C852 A856 A863 U864 U873 A881 U886 A898 G904 U912 G913 G914 A915 U928 A933 C934 U935 G936
G942 C943 A944 U945 U960 A966 C969 A970 U982 A983 G984 G987 A988 A992 A993 U996 A1003 U1004 A1005 C1006 C1007 C1016 A1020 C1021 U1024 A1025 A1026 A1027 C1028 G1032 A1039 G1040 U1052 G1053 U1057 U1058 U1059
U1060 A1061 A1062 U1063 A1076 C1077
U1080 A1081 C1082 A1086 A1091 A1092 C1096 U1097 G1100 G1109 G1110 G1111 G1112 A1113 G1114 U1115 G1126 A1138 G1150 C1158 C1159 A1160 G1164 G1167 G1170 U1185 U1191 A1194 C1195 A1196 C1197 G1198 G1199 G1200 A1204 C1205 U1206 C1207 A1208 A1217 G1218 A1219 C1220
A1226 A1227 G1228 G1229 A1230 U1231 C1235 U1239 U1240 G1241 A1242 G1243 A1244 G1245 C1246 G1255 A1256 U1257 U1258 U1259 U1260 C1274 U1285 U1286 A1287 G1288 G1294 G1295 U1301 A1312 A1313 U1314 U1315 G1316 C1317 G1318 A1319 U1320 A1321 C1332 A1337 A1341 A1344 A1345 A1346 G1354
U1361 U1362 U1363 G1364 G1367 A1371 U1378 C1389 U1390 U1398 C1399 A1410 U1413 U1414 U1415 G1419 C1420 A1421 A1422 A1427 G1428 U1432 G1438 G1445 G1448 C1451 U1452 G1453 G1458 C1459 A1460 C1461 A1471 C1472 U1473 C1481 C1482 A1483 U1489 C1490 U1491
A1492 A1493 U1496 G1506 U1514 A1515 A1516 G1523 A1524 U1535 G1536 C1537 U1538 G1539 G1540 G1541 G1542 G1553 U1554 A1555 A1556 U1557 U1558 A1559 U1560 C1568 A1569 A1573 G1574 U1582 A1583 G1584 U1585 G1590 C1591 A1592 C1596 A1597 U1598 C1599 A1600 G1601 C1606 A1614 C1615 G1616
C1619 C1620 U1621 C1632 U1650 U1657 G1658 G1663 C1664 U1665 A1678 A1681 U1682 G1685 C1686 U U A G A G A A G G G G G C A A C U C C A U C U C A1712 G1715 C1716 G1720 C1739 A1754 A1755 A1756 G1757
A1766 G1767 G1768 U1769 U1770 G1780 A1781 A1782 C1783 C1784 G1792 G1793 A1794 U1795 C1796 U1799 A1800
• Molecule 18: 18S ribosomal RNA Chain 2b:
U1 A2 U3 C4 C25 A26 U27 G34 A40 A43 U44 U45 A46 A47 G48 G57 U60 G63 A68 G69 C70 A71 A72 U73 U74 U75 A76 U77 A86 A93 A103 A104 U111 C114 G115 U116 G127 C131 U132
U133 U134 A135 C136 U137 A138
C139 A140 U141 G142 A145 U146 G153 U158 U159 C166 U177 U178 U183 C184 U185 C186 C190 C191 U192 U193 U194 G195 G196 A197 A200 A215 A218 A219 C224 A225 A226 U227 G228 U229 C230 U231 U232 C233 A236 C237 U238 C239 U240 U241 G246 C250
A417 G418 G423 C424 A425 G426 C427 A428 G434 A437 A438 U439 C444 A445 A446 U447 C448 U453 A460 A468 C469 A470 A475 C484 A485 G486 G487 G488 U493
U494 C495 G496 G497 G498 U499 C500 U501 U502 G503 U504 A505 A506 U507 U508 G509 G510 A511 A512 U513 G514
A515 C519 A527 U532 U533 A534 A535 C536 G537 A538 G539 G540 A541 A542 C543 A544 A545 G548 G551 G552 G553 C554 A555 A556 G557 U558 C559 C565 G568 C569 A570 G574 U578 A579 A580 U581 U582 A585 A594 G595 U608 U609 G610 U611 C614 A619
A620 A621 A622 A623 G624 G641 G642 G648 U649 U650 G651 G652 C653 C654 G655 G656 U657 C658 C G A U U U U U U C G U G U A C U G676 G677 A678 U679 U680 U681 C682 C683 A684 A685 C686 G687 C691 U694 U695 C696 C697 U698 U699 C700 U701
G702 G703 C704 U705 A706 A707 C708 C709 U710 U711 G712 A713 G714 U718 U719 G720 U721 G722 G723 C724 U725 C726 U727 U728 G729 G730 C731 G732 A733 A734 C735 G738 U742 U743 U744 U745 A754 A755 A756 G765 A771 A774 G775 G778 U779
A780 U781 U782 G783 C784 G787
A788 A789 U794 U795 A803 A806 A812 G815 G816 A817 C818 G819 U820 U821 G824 A829 U830 U831 U832 U833 U839 U840 U841 G846 U854 A855 A856 A863 U864 G868 C874 A892 A898 G899 U912 G913 G914 A915 U928 A933 C934 U935
G942 C943 A944 U945 A951 U959 U960 U961 C962 U965 A966 C969 A970 U982 A992 A993 U996 U1004 A1005 C1006 C1007 A1020 C1021 A1026 A1027 C1028 U1029 A1030 U1031 G1040 A1043 U1052 G1053 U1058 U1059 U1060 A1061 A1062 U1063 G1064 A1065 G1074 C1075 A1076
C1082 A1086 A1092 A1093 C1096 U1097 G1100 G1107 G1108 G1109 G1110 G1111 G1112 A1113 A1138 G1150 C1158 C1159 A1160 G1164 G1167 U1185 U1191 A1194 C1195 A1196 C1197 G1198 G1199 G1200 A1203 A1204 C1205 U1206 C1207 G1212 A1217 G1218 A1226 A1227 G1228 G1229
A1230 U1231 C1235 U1239 U1240 G1241 A1242 G1243 A1244 G1245 C1246 G1255 A1256 U1257 U1258 U1259 G1273 C1274 A1275 U1276 U1286 A1287 G1288 G1294 U1307 A1312 A1313 U1314 U1315 G1318 A1319 U1320 A1321 A1322 C1332 A1337 A1344 A1345 A1346 U1347 A1348 G1354 A1360 U1361 U1362 U1363
G1364 G1367 A1371 U1372 U1378 G1385 G1386 G1387 A1388 C1389 U1390 A1391 U1398 C1399 U1413 U1414 U1415 C1426 A1427 G1428 U1432 G1445 A1446 C1447 G1448 C1451 U1452 G1453 G1454 G1455 C1456 C1457 G1458 C1459 A1460 C1467 A1471 C1472 U1473 C1481 C1482 G1486 U1489 C1490 U1491
A1492 A1493 C1494 C1495 U1496 G1506 U1514 A1515 A1516 U1517 G1521 U1522 G1523 A1524 U1535 G1536 C1537 U1538 G1553 U1554 A1555 A1556 U1557 U1558 A1559 U1560 C1568 A1569 G1572 A1573 G1574 G1575 G1584 U1585 G1590 C1596 A1597 U1598 C1599 A1600 G1601 G1616 C1619 C1620 U1621 G1622 C1632
U1657 G1658 G1663 C1664 U1665 C1674 A1678 G1679 G1680 A1681 U1682 G1685 C1686
U U A G A G A A G G G G G C A A C U C C A U C U C A1712 G1715 C1716 G1720 G1736 U1742 A1750 A1754 A1755 A1756 G1757
A1766 G1767 G1768 U1769 U1770 U1771 C1772 G1780 A1781 A1782 G1792 G1793 A1794 U1795 C1796 U1799 A1800
• Molecule 19: 40S ribosomal protein S0-A Chain P: S2 V31 D43 G44 I111 G166 L184 R185 G186 P207
Chain Pb: S2 V31 G94 F107 I111 T112 R113 R119 G166 L184 R185 G186 P207
• Molecule 20: 40S ribosomal protein S1-A Chain Q: V20 V21 R41 L54 D59 A60 G63 G75 G93 Q146 S169 L218 K219 K222 G233 E234 G235
• Molecule 20: 40S ribosomal protein S1-A Chain Qb: V20 L54 D59 Q146 K151 K202 E234 G235
• Molecule 21: 40S ribosomal protein S2 Chain R: G34 G75 R91 D106 P236 Q250
• Molecule 21: 40S ribosomal protein S2 Chain Rb: G34 G75 R91 D106 P236 Q250
• Molecule 22: 40S ribosomal protein S3 Chain A: A3 T44 K45 R51 R90 L157 V164 K194 S195 R196 E213 E214 E215 P216 I217 L218 A219 Y225
• Molecule 22: 40S ribosomal protein S3 Chain Ab:
A3 T44 K45 N62 R90 R143 R178 Q179 G180 R196 T197 G198 K212 E213 I217 L218 A219 Y225 • Molecule 23: 40S ribosomal protein S4-A Chain S: A2 D88 V89 E118 D163 F175 I195 Q231 G260 L261
• Molecule 23: 40S ribosomal protein S4-A Chain Sb: A2 H16 A28 E40 A110 V111 T115 D116
E117 E118 Y149
D163 S166 D171 F175 D176 A177 I195 S213 L214 D215 Q231 G232 K240 G260 L261
• Molecule 24: 40S ribosomal protein S5 Chain B: F20 L25 I29 P30 E31 E50 V51 E52 H72 N100 G101 D126 G150 A154 R157 R225 • Molecule 24: 40S ribosomal protein S5 Chain Bb:
F20 L25 I29 N44 A71
L93 N100 G101 D126 G152 R225
• Molecule 25: 40S ribosomal protein S6-A Chain T:
M1 D37 G38 Y48 V49 L68 R98 K116
L121 L124 E218
• Molecule 25: 40S ribosomal protein S6-A Chain Tb:
M1 V67 L68 R94 R98 R132 K164 G165 E166
R191 E218
Chain U: A3 L9 S30 S31 A52 G53 V64 P65 Q74 K111 V130 F131 S187
• Molecule 26: 40S ribosomal protein S7-A Chain Ub: A3 L9 S31 V64 P65 Q74 L81 K111 V130 F131 E134 S187
• Molecule 27: 40S ribosomal protein S8-A Chain V:
MET G2 I60 K123 LYS LYS ASN VAL LYS GLU GLU GLU THR VAL ALA K135 A148
K200
• Molecule 27: 40S ribosomal protein S8-A Chain Vb:
MET G2 I60 R77 K123
LYS LYS ASN VAL LYS GLU GLU GLU THR VAL ALA K135 I152
A198
K199 K200
• Molecule 28: 40S ribosomal protein S9-A Chain W:
P2 R3 P163 K180 A181 E182
E186
• Molecule 28: 40S ribosomal protein S9-A Chain Wb: P2 R3 A4 S22 E88 D89 K90 K91 A98 G137 K138 D151 K154 S162 P163 R174 A177 A178 R179 K180 A181 E182 A183 S184 G185 E186
• Molecule 29: 40S ribosomal protein S10-A Chain C:
M1 K24 K31 E34 I35 I92
• Molecule 29: 40S ribosomal protein S10-A Chain Cb:
M1 A23 K24 E34 I35 I86
I92
• Molecule 30: 40S ribosomal protein S11-A Chain X: S2 T3 E4 L5 T6 K26 T27 S28 K29 R30 K46 R67 R116 I122 R129 A144 A145 A146 G147
• Molecule 30: 40S ribosomal protein S11-A Chain Xb: S2 T3 E4 L5 K29 R30 R67 R116 V117 A144 A145 A146 G147
• Molecule 31: 40S ribosomal protein S12 Chain D: A20 E21 V22 T23 I24 E25 T67 E68 I89 R108 E109 G110 N111 S119 E129 T130 D131 Q142 Q143
• Molecule 31: 40S ribosomal protein S12 Chain Db: A20 E21 V22 T23 I24 E25 L45 I89 K90 D107 R108 E109 G110 N111 E129 T130 D131 H139 F140 S141 Q142 Q143
• Molecule 32: 40S ribosomal protein S13 Chain Y: G2 A22 S29 A144 N151
• Molecule 32: 40S ribosomal protein S13 Chain Yb:
G2 A22 S29 E83 A133 N151
• Molecule 33: 40S ribosomal protein S14-B Chain Z: N10 S11 Q65 D66 A69 K70 E73 I115 R133 L137
• Molecule 33: 40S ribosomal protein S14-B Chain Zb:
N10 V79 P122 L137
• Molecule 34: 40S ribosomal protein S15 Chain E: T14 P68 E71 T124 P125 V126 G129 R130 A131 G132
• Molecule 34: 40S ribosomal protein S15 Chain Eb:
T14 R40 M49
P68 E69 N70 E71 T124 P125 V126 R127 A131
G132
• Molecule 35: 40S ribosomal protein S16-A Chain F:
A3
E40 T115 L116 R143
• Molecule 35: 40S ribosomal protein S16-A Chain Fb: A3 V4 E40 K102 R114 T115 L116 R143
Chain G: G2 R5 E37 G68 D82 K116 I122 N123 V124 S125 A126
• Molecule 36: 40S ribosomal protein S17-A Chain Gb: G2 R5 D82 E87 N123 V124 S125 A126
• Molecule 37: 40S ribosomal protein S18-A Chain H:
S2 N90 D91 I92 L101 A146
• Molecule 37: 40S ribosomal protein S18-A Chain Hb:
S2 L3
H13 I14 E60 N90 D91 I92 L101 R123 A146
• Molecule 38: 40S ribosomal protein S19-A Chain I:
P2 E144
• Molecule 38: 40S ribosomal protein S19-A Chain Ib:
P2 G32
I116 E144
• Molecule 39: 40S ribosomal protein S20 Chain J:
I19 V51 K52
• Molecule 39: 40S ribosomal protein S20 Chain Jb:
I19 L50 V51 P71 N72 A119
• Molecule 40: 40S ribosomal protein S21-A Chain a:
M1 G6 D27
N81 R87
• Molecule 40: 40S ribosomal protein S21-A Chain ab: M1 K5 L8 V9 E10 L11 D27 R44 R87
• Molecule 41: 40S ribosomal protein S22-A Chain b:
T2 W89 L93 Y130
• Molecule 41: 40S ribosomal protein S22-A Chain bb:
T2 L11 R68 W89 L93 L104 Y130
• Molecule 42: 40S ribosomal protein S23-A Chain c:
G2 K3 R7 R13 R16 S41 P88 N89 E101 R144
S145
• Molecule 42: 40S ribosomal protein S23-A Chain cb:
G2 S41 K56 P88 N89 E101 R109 E138 E141 K142 P143 R144 S145
• Molecule 43: 40S ribosomal protein S24-A Chain d:
S2
D3
A33 K49 A50 E51 K52 F58 S78 E98
D135
• Molecule 43: 40S ribosomal protein S24-A Chain db: S2 D3 A4
H29 A33 N34 V35 K49 A50 E51
K52 F58 S78 A97 E98 K99 V100 E101 R108 F119 K123 D135 • Molecule 44: 40S ribosomal protein S25-A Chain K:
Q37
T105
• Molecule 44: 40S ribosomal protein S25-A Chain Kb:
Q37 G87 T105
• Molecule 45: 40S ribosomal protein S26-B Chain e:
P2 S7 N8 G9 R10 K34 E61 Y62 C74 P98
• Molecule 45: 40S ribosomal protein S26-B Chain eb:
P2 R5 A6 S7 R10 K34 A35 E61 Y62 C74 P98
Chain f: V2 A53
I62 K82
• Molecule 46: 40S ribosomal protein S27-A Chain fb:
V2 I62 K82
• Molecule 47: 40S ribosomal protein S28-A Chain L: T5 P6 V7 N51 L66 R67
• Molecule 47: 40S ribosomal protein S28-A Chain Lb: T5 P6 R67
• Molecule 48: 40S ribosomal protein S29-A Chain M: E4 N5 V6 H10 R13 Y14 T28 I50 R56
• Molecule 48: 40S ribosomal protein S29-A Chain Mb: E4 N5 V6 H10 P11 R12 R13 Y14 S18 T28 I50 R56
• Molecule 49: 40S ribosomal protein S30-A Chain g: A2 K3 V4 H5 T48 S61
• Molecule 49: 40S ribosomal protein S30-A Chain gb: A2 K3 V4 H5 G6 S7 L8 L39 N46 V47 T48 L49 V50 N51 P60 S61
• Molecule 50: Ubiquitin-40S ribosomal protein S31 Chain N: R80 K81 V84 L103 Y106 K107 V108 D109 A110 E111 S122 N123 P124 L139 V147 A152
• Molecule 50: Ubiquitin-40S ribosomal protein S31 Chain Nb: R80 K89 K92 L103 Y106 A110 E111 P124 V147 A152
• Molecule 51: Guanine nucleotide-binding protein subunit beta-like protein Chain O: L7 D51 N148 E160 A161 A162 D163 D164 D165 G194 H195 N196 L234 S235 A236 Q237 A257 F263 D266 P267 Q268 D272 D273 E277 F278 A279 K283 A318 N319
• Molecule 51: Guanine nucleotide-binding protein subunit beta-like protein Chain Ob: L7 D163 K229 A318 N319
• Molecule 52: 60S ribosomal protein L17-A Chain AX:
MET A2 A156 VAL ALA LYS ALA ALA GLU LYS LYS V165 A184
• Molecule 52: 60S ribosomal protein L17-A Chain XX:
MET A2 A156 VAL ALA LYS ALA ALA GLU LYS LYS V165 A183
A184
• Molecule 53: 60S ribosomal protein L18-A Chain BB: G2 V84 K98 T99 K159 V186
• Molecule 53: 60S ribosomal protein L18-A Chain YB:
G2 D41 K98 R179 V186
• Molecule 54: 60S ribosomal protein L19-A Chain BF: A2 G16 N36 R42 K52 R181 D182 A183
L184 LEU LYS GLU ASP ALA
• Molecule 54: 60S ribosomal protein L19-A Chain YF: A2 E187 D188 A189
• Molecule 55: 60S ribosomal protein L20-A Chain BH: M1 E17 E130 Y172
• Molecule 55: 60S ribosomal protein L20-A Chain YH: M1 S18 S85 E130 A133 D134 Y172
Chain BJ: G2 A24 G37 G81 R92 R130 P135 R136 R139 G145 T158 F159 I160
• Molecule 56: 60S ribosomal protein L21-A Chain YJ: G2 H22 G23 A24 N77 R88 P135 R136 I160
• Molecule 57: 60S ribosomal protein L22-A Chain BL: I11 N49 D59 Y108
• Molecule 57: 60S ribosomal protein L22-A Chain YL:
I11 N52
Y108
• Molecule 58: 60S ribosomal protein L23-A Chain AB: N4 G5 G41 V137
• Molecule 58: 60S ribosomal protein L23-A Chain XB: N4 G43 T115 V135 V136 V137
• Molecule 59: 60S ribosomal protein L24-A Chain AE: M1 D25 T64 E65 E66 V67 A68 K69 K70 R71 S72 R73 K74 T75 V76 K77 P81 I82 T83 G84 A85 S86 L87 D88 L89 I90 K91 E92 R93 R94 S95 L96 K97 P98 E99 V100 R101 K102 A103 N104 R105 E106 E107 K108 L109 K110 A111 E114 K115 K116 K117 A118 A121 A122 R123
K124 A125 E126 K127 A128 K129 S130 A131 G132 T133 Q134 S135
• Molecule 59: 60S ribosomal protein L24-A Chain XE: M1 R30 T64 E65 E66 V67 A68 K69 K70 K74 T75 A78 Q79 R80 P81 I82 A85 S86 L87 S95 L96 K97 P98 E99 V100 R101 K102 A103 E106 A125 E126 K127 A128 K129 S130 A131 G132 T133 Q134 S135
• Molecule 60: 60S ribosomal protein L25 Chain AH: A23 D129 I142
• Molecule 60: 60S ribosomal protein L25 Chain XH:
A23 I142
• Molecule 61: 60S ribosomal protein L26-A Chain AK:
A2 D7
K125
• Molecule 61: 60S ribosomal protein L26-A Chain XK:
There are no outlier residues recorded for this chain. • Molecule 62: 60S ribosomal protein L27-A
Chain AN: A2 A91
F101 E102 F136
• Molecule 62: 60S ribosomal protein L27-A Chain XN:
A2 K3
F101 E102 L134
R135 F136
• Molecule 63: 60S ribosomal protein L28 Chain AR:
P2 G18 A66 L78 E84
A149
• Molecule 63: 60S ribosomal protein L28 Chain XR:
P2 A17 L78 A149
• Molecule 64: 60S ribosomal protein L29 Chain AV: A2 N19 G20 I21 K22 K23 P24 R40 A56 A57 K58 K59
• Molecule 64: 60S ribosomal protein L29 Chain XV: A2 N19 G20 I21 A56 A57 K58 K59
• Molecule 65: 60S ribosomal protein L30 Chain AY: S6 Q7 E8 T102 T103 L104 A105
• Molecule 65: 60S ribosomal protein L30 Chain XY: S6 Q7 Q71 L84 D97 T103 L104 A105
Chain BC: L4 V7 F90 E111
D112
• Molecule 66: 60S ribosomal protein L31-A Chain YC: L4 V7 R28 E82 E83 F90 E111 D112
• Molecule 67: 60S ribosomal protein L32 Chain BG: A2 H6 V10 K11 F17 K34 Q35 K36 Q52 R125 L128
• Molecule 67: 60S ribosomal protein L32 Chain YG: A2 H6 P7 K8 F17 A127 L128
• Molecule 68: 60S ribosomal protein L33-A Chain BK:
There are no outlier residues recorded for this chain. • Molecule 68: 60S ribosomal protein L33-A
Chain YK:
There are no outlier residues recorded for this chain. • Molecule 69: 60S ribosomal protein L34-A
Chain BN: A2 R8 L38 A39 E107 Q108 T109 E110 A111 A112 K113
• Molecule 69: 60S ribosomal protein L34-A Chain YN:
A2 R8 R16 K37 C44 A112
K113
• Molecule 70: 60S ribosomal protein L35-A Chain BP:
A2 Q20 K83 A91 L92
A120
• Molecule 70: 60S ribosomal protein L35-A Chain YP: A2 D79 K83 A91 A120
• Molecule 71: 60S ribosomal protein L36-A Chain AC: T2 A48 N63 R98 R99 H100
• Molecule 71: 60S ribosomal protein L36-A Chain XC: T2 N63 R68 R99 H100
• Molecule 72: 60S ribosomal protein L37-A Chain AF:
There are no outlier residues recorded for this chain. • Molecule 72: 60S ribosomal protein L37-A
Chain XF: G2 C22 C37 R73 F74 K75 A83
• Molecule 73: 60S ribosomal protein L38 Chain AI:
A2 R17 K33 A59 L78
• Molecule 73: 60S ribosomal protein L38 Chain XI:
A2 R17 K33 T72
L78
• Molecule 74: 60S ribosomal protein L39 Chain AL:
A2 I51
• Molecule 74: 60S ribosomal protein L39 Chain XL:
There are no outlier residues recorded for this chain. • Molecule 75: Ubiquitin-60S ribosomal protein L40 Chain AO:
I77
C99 R113 K128
• Molecule 75: Ubiquitin-60S ribosomal protein L40 Chain XO: I77 K83 C91 D92 R106 A107 T108 K126 L127 K128
• Molecule 76: 60S ribosomal protein L41-B Chain AS:
There are no outlier residues recorded for this chain. • Molecule 76: 60S ribosomal protein L41-B
M1 R2 A3 R9 R23 S24 K25
• Molecule 77: 60S ribosomal protein L42-A Chain AP: V2 C74 G95 E96 Q99 K100 F106
• Molecule 77: 60S ribosomal protein L42-A Chain XP: V2 T7 G94 Q105 F106
• Molecule 78: 60S ribosomal protein L43-A Chain AT: A2 C59 A74 V90 E91 A92
• Molecule 78: 60S ribosomal protein L43-A Chain XT: A2 R49 C57 E91 A92
• Molecule 79: 60S acidic ribosomal protein P0 Chain BU: MET GLY G3 R16 E20 E21 Y22 K23 N32 R61 R64 G65 F66 L67 S68 D69 L70 P71 D72 F73 E74 K75 L76 L77 P78 F79 V80 K81 N90 E91 P92 L93 A107
PRO ALA ARG ALA GLY ALA VAL ALA PRO GLU ASP ILE TRP VAL ARG ALA VAL ASN THR GLY
MET GLU PRO GLY LYS THR SER PHE PHE GLN ALA LEU GLY VAL PRO THR LYS ILE ALA ARG GLY THR ILE GLU ILE VAL SER ASP VAL LYS VAL VAL ASP ALA GLY ASN LYS VAL GLY GLN SER GLU ALA SER LEU LEU ASN LEU LEU ASN ILE SER PRO PHE THR PHE G184 Q189
V190 Y191 D192 N193 G194 Q195 V196 F197 P198 S199 S200 I201 L202 H212 A216
VAL SER THR ILE ALA SER ILE SER LEU ALA ILE GLY TYR PRO THR LEU PRO SER VAL GLY HIS THR LEU ILE ASN ASN TYR LYS ASP LEU LEU ALA VAL ALA ILE ALA ALA SER TYR HIS TYR PRO GLU ILE
• Molecule 79: 60S acidic ribosomal protein P0 Chain YU: MET GLY G3 A9 E10 R16 E20 E21 Y22 K23 S24 D31 N32 R64 G65 F66 L67 S68 D69 L70 P71 D72 F73 E74 K75 L76 L77 P78 F79 V80 K81 N90 E91 P92 L93 T94 E95 A106 A107
PRO ALA ARG ALA GLY ALA VAL ALA PRO GLU ASP ILE TRP VAL
ARG ALA VAL ASN THR GLY MET GLU PRO GLY LYS THR SER PHE PHE GLN ALA LEU GLY VAL PRO THR LYS ILE ALA ARG GLY THR ILE GLU ILE VAL SER ASP VAL LYS VAL VAL ASP ALA GLY ASN LYS VAL GLY GLN SER GLU ALA SER LEU LEU ASN LEU LEU ASN ILE SER PRO PHE
THR PHE G184 V190 Y191 D192 N193 G194 Q195 V196 F197 P198 S199 S200 I201 L202 D203 I204 A216
VAL SER THR ILE ALA SER ILE SER LEU ALA ILE GLY TYR PRO THR LEU PRO SER VAL GLY HIS THR LEU ILE ASN ASN TYR LYS ASP LEU LEU ALA VAL ALA ILE ALA ALA SER TYR
HIS TYR PRO GLU ILE GLU ASP LEU VAL ASP ARG ILE GLU ASN PRO GLU LYS TYR ALA ALA ALA ALA PRO ALA ALA THR SER ALA ALA SER GLY ASP ALA ALA PRO ALA GLU GLU ALA ALA ALA GLU GLU GLU GLU GLU SER ASP ASP ASP MET GLY PHE GLY LEU PHE ASP • Molecule 80: P-site tRNA
Chain n:
U1 U16 G17 G18 U19 C22 G23 G24 U33 C39 C40 A41 G42 U48 C56 A57 A58 G59 G67 G73 C74 C75 A76 • Molecule 81: E-site tRNA
Chain m: U1 U6 A7 U8 A9 G10 C11 G12 U13 C16 G17 G18 C19 U20 C25 C28 A29 C30 G31 C32 U33 U34 U35 C36 A37 C38 C39 G40 U41 G42 G43 A44 G45 A46 C47 C48 U53 C60 C61 C62 C63 G64 U65 A66 U67 G72 C73 C74 A75 • Molecule 82: A/P hybrid tRNA
Chain nb: G1 C4 A9 C13 A14 G15 U16 C17
G18 G19 U20 A21 G22 A23 G24 C25 G30 U33 A34 G35 G36 A37 A38 U39 C40 C41 C42 C43 G44 U47 C48 C49 U50 U51 G52 G53 U54 U55 C56 G57 A58 U59 U60 C68 C72 A73 C74 C75 A76 • Molecule 83: P/E hybrid tRNA
Chain mb: C1 G2 G9 C17
U18A G19 G20 U21 A36 U48
C49 C57 C63 C76 A77 • Molecule 84: mRNA
U4 U5 U6 U7 U8 U9 U10 U11 U12 U13 G16 A19 U23 U24 U25 U26 U27 U28 U29 U30 U31 A32 A33 U34 C35
G38 G39 A40
4 Experimental information
O
iProperty Value Source
EM reconstruction method SINGLE PARTICLE Depositor
Imposed symmetry POINT, C1 Depositor
Number of particles used 15739 Depositor
Resolution determination method FSC 0.143 CUT-OFF Depositor
CTF correction method PHASE FLIPPING AND AMPLITUDE
CORRECTION Depositor
Microscope FEI TITAN KRIOS Depositor
Voltage (kV) 300 Depositor
Electron dose (e−/Å2) 2.5 Depositor
Minimum defocus (nm) Not provided
Maximum defocus (nm) Not provided
Magni cation Not provided
Image detector FEI FALCON II (4k x 4k) Depositor
Maximum map value 0.160 Depositor
Minimum map value -0.067 Depositor
Average map value 0.000 Depositor
Map value standard deviation 0.006 Depositor
Recommended contour level 0.013 Depositor
Map size (Å) 758.7976, 758.7976, 758.7976 wwPDB
Map dimensions 506, 506, 506 wwPDB
Map angles (◦) 90.0, 90.0, 90.0 wwPDB
5 Model quality
O
i5.1 Standard geometry
O
iBond lengths and bond angles in the following residue types are not validated in this section: ZN
The Z score for a bond length (or angle) is the number of standard deviations the observed value is removed from the expected value. A bond length (or angle) with |Z| > 5 is considered an outlier worth inspection. RMSZ is the root-mean-square of all Z scores of the bond lengths (or angles).
Mol Chain RMSZBond lengths#|Z| >5 RMSZBond angles#|Z| >5
1 BQ 1.05 1/74873 (0.0%) 1.11 138/116727 (0.1%) 1 YQ 1.24 39/74873 (0.1%) 1.21 228/116727 (0.2%) 2 BR 0.92 0/2883 1.03 2/4491 (0.0%) 2 YR 1.05 0/2883 1.12 6/4491 (0.1%) 3 BS 1.04 1/3724 (0.0%) 1.10 5/5798 (0.1%) 3 YS 1.25 3/3724 (0.1%) 1.20 15/5798 (0.3%) 4 AW 0.63 0/1946 0.71 0/2614 4 XW 0.78 1/1946 (0.1%) 0.78 0/2614 5 BA 0.62 0/3146 0.78 4/4228 (0.1%) 5 YA 0.71 1/3146 (0.0%) 0.83 3/4228 (0.1%) 6 BE 0.62 0/2800 0.79 5/3790 (0.1%) 6 YE 0.73 0/2800 0.82 3/3790 (0.1%) 7 BI 0.50 0/2408 0.72 3/3248 (0.1%) 7 YI 0.56 0/2408 0.72 2/3248 (0.1%) 8 BM 0.50 0/1269 0.81 2/1705 (0.1%) 8 YM 0.52 0/1269 0.79 0/1705 9 BO 0.65 0/1828 0.78 1/2461 (0.0%) 9 YO 0.73 0/1828 0.76 0/2461 10 AA 0.48 0/1795 0.58 0/2429 10 XA 0.53 0/1795 0.61 0/2429 11 AD 0.48 0/1531 0.60 0/2062 11 XD 0.54 0/1531 0.62 0/2062 12 BD 0.53 0/1732 0.73 0/2323 12 YD 0.65 0/1732 0.80 1/2323 (0.0%) 13 AG 0.43 0/1374 0.70 0/1842 13 XG 0.48 0/1374 0.69 0/1842 14 AJ 0.55 0/1573 0.73 1/2113 (0.0%) 14 XJ 0.63 0/1573 0.78 1/2113 (0.0%) 15 AM 0.47 0/1074 0.68 0/1446 15 XM 0.50 0/1074 0.68 1/1446 (0.1%) 16 AQ 0.67 0/1757 0.74 0/2354 16 XQ 0.83 0/1757 0.81 0/2354
Mol Chain RMSZBond lengths#|Z| >5 RMSZBond angles#|Z| >5 17 AU 0.63 0/1585 0.61 0/2128 17 XU 0.73 0/1585 0.63 1/2128 (0.0%) 18 2 1.65 14/41891 (0.0%) 1.20 147/65273 (0.2%) 18 2b 1.68 14/41891 (0.0%) 1.27 200/65273 (0.3%) 19 P 0.45 0/1623 0.67 1/2222 (0.0%) 19 Pb 0.56 0/1623 0.72 1/2222 (0.0%) 20 Q 0.46 0/1748 0.65 1/2352 (0.0%) 20 Qb 0.54 0/1748 0.66 0/2352 21 R 0.52 0/1665 0.62 0/2263 21 Rb 0.63 0/1665 0.70 0/2263 22 A 0.47 0/1759 0.79 1/2368 (0.0%) 22 Ab 0.58 0/1759 0.85 0/2368 23 S 0.48 0/2109 0.65 0/2839 23 Sb 0.50 0/2109 0.69 0/2839 24 B 0.47 0/1629 0.82 0/2202 24 Bb 0.51 0/1629 0.88 1/2202 (0.0%) 25 T 0.42 0/1779 0.73 3/2379 (0.1%) 25 Tb 0.44 0/1779 0.71 3/2379 (0.1%) 26 U 0.44 0/1511 0.83 1/2036 (0.0%) 26 Ub 0.52 0/1511 0.84 2/2036 (0.1%) 27 V 0.49 0/1514 0.65 0/2021 27 Vb 0.48 0/1514 0.65 0/2021 28 W 0.48 0/1519 0.65 0/2035 28 Wb 0.53 0/1519 0.70 1/2035 (0.0%) 29 C 0.43 0/757 0.57 0/1022 29 Cb 0.49 0/757 0.61 0/1022 30 X 0.61 0/1194 0.67 0/1610 30 Xb 0.61 0/1194 0.65 0/1610 31 D 0.32 0/898 0.67 0/1220 31 Db 0.35 0/898 0.69 2/1220 (0.2%) 32 Y 0.56 0/1215 0.66 0/1638 32 Yb 0.58 0/1215 0.66 0/1638 33 Z 0.46 0/960 0.71 1/1290 (0.1%) 33 Zb 0.56 0/960 0.71 0/1290 34 E 0.38 0/959 0.68 0/1288 34 Eb 0.47 0/959 0.73 1/1288 (0.1%) 35 F 0.45 0/1125 0.66 0/1510 35 Fb 0.55 0/1125 0.70 0/1510 36 G 0.43 0/1011 0.73 0/1355 36 Gb 0.49 0/1011 0.72 0/1355 37 H 0.37 0/1211 0.67 0/1628 37 Hb 0.45 0/1211 0.71 1/1628 (0.1%) 38 I 0.42 0/1130 0.60 0/1517
