Caco-2
Tick-borne encephalitis virus replication, intracellular trafficking, and pathogenicity in human intestinal Caco-2 cell monolayers.
... However, after prolonged incubation time up to 5 days p.i. apoptosis ratio was massively increased in infected cell monolayers. Concomitantly, TER decreases after 4 days and was lowered to the half of the initial value ...
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Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells.
... Flow cytometry was used to measure DNA ploidy as well as alterations in cell-cycle profiles char- acteristic of DNA fragmentation (necrosis) compared to patterned DNA cleavage (apoptosis). In this way, we were able to ...
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Avaliação da atividade antioxidante do extrato hidroalcoólico da romã (Punica granatum, L.) sobre células da linhagem Caco-2.
... TRIADOU, N.; DUSSAULX, E.; LACROIX, B.; SIMON- ASSMAN, P.; HAFFEN, K.; FOGH, J.; ZWEIBAUM, A. Enterocyte- like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. ...
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RESPOSTA CELULAR À INFECÇÃO POR Campylobacter. jejuni EM EMBRIÕES E EXPLANTS DE ÍLEO DE AVES E PARTICIPAÇÃO DO CITOESQUELETO E LISOSSOMOS NO PROCESSO DE INVASÃO EM CÉLULAS CACO-2
... in Caco-2 cells and the cellular response by quantifying the expression of inflammatory ...in Caco-2 cells is dependent on actin microfilaments and tubulin and to internalize the bacteria ...
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Influence of Gastrointestinal System Conditions on Adhesion of Exopolysaccharide-producing Lactobacillus delbrueckii subsp. bulgaricus strains to Caco-2 Cells
... Dairy L. delbrueckii subsp. bulgaricus and Streptococcus thermophilus are the microorganisms extensively used as the starter bacteria for the manufacture of yogurt (Radke- Mitchell and Sandine, 1986; Siitonen et al., ...
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Gliadin peptides induce tissue transglutaminase activation and ER-stress through Ca2+ mobilization in Caco-2 cells.
... Ca 2+ concentration increased in Caco-2 cells also in the absence of extracellular Ca 2+ ions demonstrates that gliadin peptides induced Ca 2+ mobilization from intracellular Ca ...
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Tumor necrosis factor alpha increases epithelial barrier permeability by disrupting tight junctions in Caco-2 cells
... proteins. Caco-2 cells were plated onto Transwell ® microporous filters and treated with TNF-α (10 or 100 ng/mL) for 0, 4, 8, 16, or 24 ...of Caco-2 intestinal ...in Caco-2 ...
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Benzotropolone moiety in theaflavins is responsiblefor inhibitingpeptide-transport and activating AMP-activated protein kinase in Caco-2 cells
... in Caco-2 cells, although mechanisms underlying LKB1 activation by either transforming growth factor- β-activated kinase (TAK-1) [23-25] or Sirtuin 1 (SIRT1) [26, 27] in Caco-2 cells remain ...
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HYPOXIC STRESS, HEPATOCYTES AND CACO-2 VIABILITY AND SUSCEPTIBILITY TO Shigella flexneri INVASION
... 3. Hypoxic conditions: Both hepatocytes and Caco-2 cells were cultured for 7-8 days. The experimental groups are referred to as hypoxia (H) and normoxia (N). Another two experimental groups were formed: ...
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Avaliação in vitro da Toxicidade da Clomipramina e do Hipericão nas Linhas Celulares HepG2 e Caco-2
... e Caco-2 com clomipramina e extracto de hipericão de forma isolada ou em conjunto pode ser citotóxica para ambas as linhas celulares, dependendo quer da sua concentração quer do tempo de exposição aos ...
135
Effect of Erica australis extract on Caco-2 cells, fibroblasts and selected pathogenic bacteria responsible for wound infection
... Title: Effect of Erica australis extract on CACO-2 cells, fibroblasts and selected 1 .. pathogenic bacteria responsible for wound infection 2 .[r] ...
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Desenvolvimento e validação de um método analítico para determinação de anti-hipertensivos em amostras de estudos de permeação em células caco-2 utilizando cromatografia líquida acoplada à espectrometria de massas
... Essa dissertação teve como objetivo desenvolver um método para determinação simultânea de diferentes anti-hipertensivos, hidroclorotiazida, losartan, telmisartan e valsartan, e alguns de seus metabólitos losartan ...
84
In vitro cytotoxicity of ticlopidine in Hep G2 and Caco 2 cell lines
... Obesity is a chronic disease in which body fat excessively accumulated is capable of affecting the health status. It has a high prevalence worldwide and the WHO declared it a global epidemic of the XXI century. The ...
136
Quercetin represses apolipoprotein B expression by inhibiting the transcriptional activity of C/EBPβ.
... Quercetin inhibits the C/EBPβ-mediated activation of the apoB promoter Because C/EBPβ regulates the intestinal expression of apoB among C/EBP family proteins [16], we investigated the effect of C/EBPβ on the ...
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Poly(alkylidenimine) Dendrimers Functionalized with the Organometallic Moiety [Ru(η
... The 3-(4,5-dimethylthiazol-2yl)2,5-diphenyltetrazolium bromide (MTT) assay was used to explore the in vitro cytotoxic potential of the metallodendrimers 3 and 4. This assay is based on the principle that only cells that ...
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The bacterial cytoskeleton modulates motility, type 3 secretion, and colonization in Salmonella.
... Two-component regulatory systems are vital in sensing environ- mental and cell surface signals, enabling bacteria to rapidly adapt to ever changing conditions [53,54]. These signals are detected by histidine protein ...
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Exogenous HIV-1 Nef upsets the IFN-γ-induced impairment of human intestinal epithelial integrity.
... For Nef entry detection, Caco-2 cells were seeded on polycar- bonate inserts and after 21 days were treated basolaterally with biotynilated-Nef (DIATHEVA s.r.l) in the presence or absence of IFN-c for 1, 3, ...
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CoCl2, a mimic of hypoxia, induces formation of polyploid giant cells with stem characteristics in colon cancer.
... and Caco-2 cells were preincubated for 48 hours in the presence or absence of CoCl 2 for PGCC enrichment and then they were exposed for 72 h to different doses of 5-fluorouracil (A) or oxaliplatin ...
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Antibodies against Marinobacter algicola and Salmonella typhimurium flagellins do not cross-neutralize TLR5 activation.
... by Caco-2 cells. Caco-2 cells were incubated with a premix of 3 ml (grey bars) or 0 ml (black bars) of anti-flagellin hyperimmune serum with MA or STF ...
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Mechanisms of Intestinal Serotonin Transporter (SERT) Upregulation by TGF-β1 Induced Non-Smad Pathways.
... In addition to elucidation of important SERT regulatory mechanisms in the IECs, our find- ings for the first time provide feasibility of utilizing 3D-culture system of Caco-2 cells for study- ing endogenous ...
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