Culture conditions
Toxigenic potential of Aspergillus flavus tested in different culture conditions
... The objective of the present work was to evaluate the capacity of three isolates of Aspergillus flavus to produce aflatoxin under different culture conditions. This experiment was based on a 2³ factorial ...
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MATERIALS AND METHODS Organism and Culture Conditions
... growth conditions (38°C, pH ...the culture was green-brown with phycobiliproteins constituting up to 10% of the total protein, while at pH ...the culture was brownish-black and the content of these ...
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Effect of Three Different Types of Culture Conditions on
... of culture conditions with four replicates were studied: 1) manual aeration with natural sunlight; 2) manual aeration with artificial light, and; 3) constant aeration using an aquarium compressor and ...
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MATERIALS AND METHODS Organism and culture conditions
... chemical conditions are maintained homogenous and more oxygen becomes available ...the culture conditions of polypore Antrodia cinnamomea an agitation of 100 rpm increased the mycelial growth ...
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Biomass production by Arthrospira platensis under different culture conditions
... of culture. During the cyanobacterium culture, these parameters either increased or did not change, depending on the culture conditions; however, there was no significant effect on the NaHCD 3 ...
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Culture conditions affect cardiac differentiation potential of human pluripotent stem cells.
... of culture conditions have been described for the undifferentiated growth of ...different culture conditions: on mouse embryonic fibroblast (MEF) and SNL feeder cell layers together with ...
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MATERIALS AND METHODS Microorganism and Culture Conditions
... the culture containing ...supplemented culture, the rise in GLA content can be attributed to the controlled intake of oil supplement and subsequent utilization of precursor component (linoleic acid) for GLA ...
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Enhancing inulinase yield by irradiation mutation associated with optimization of culture conditions
... A new inulinase-producing strain was isolated from rhizosphere soils of Jerusalem artichoke col- lected from Shihezi (Xinjiang, China) using Jerusalem artichoke power (JAP) as sole carbon source. It was identified as an ...
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MATERIALS AND METHODS Microorganism maintenance and culture conditions
... São Paulo, Brazil), and 50 – 200 mg.L 1 of 2,5 xylidine (SIGMA, St. Louis, USA) or 20 – 50 g.L 1 of ethanol (Isofar, Rio de Janeiro, Brazil). The flasks were incubated at 28°C in the dark and shaken at 140 rpm. In ...
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MATERIALS AND METHODS Bacterial Strains and culture conditions
... 5188-5242;Agilent) in Sure hyb Chambers (Agilent) at 65º C for 16 hours. Hybridized slides were washed using Agilent Gene Expression wash buffers (Part No: 5188-5327). The hybridized, washed microarray slides were then ...
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MATERIALS AND METHODS Culture Conditions
... the Ministério da Saúde – (Brazil), which regulates toxin concentration and cyanobacteria blooms in fresh water in Brazil. In the case of the samples containing the genus Oscillatoria no toxin was detected above the ...
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MATERIAL AND METHOD Plant material and culture conditions
... In this study, an efficient method for regenerating plants from nodal cultures of seedlings was developed for Aspidosperma polyneuron. Mature seeds were surface-sterilized and embryos were germinated in Woody Plant ...
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MATERIALS AND METHODS Bacterial strains, bacteriophage, media and culture conditions
... dye in the bacterial cells, the sample was agitated for 5 min at 25 o C, followed by the application of non ablative laser (diode, wavelength 660nm, power 50mW, 2 mm spot under continuous wave –cw). A pilot test was ...
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1A gene in Metarhizium anisopliae and Metarhizium acridum across different culture conditions
... different culture media, the average Cq value of conidia collected at 24 h was considered the control treatment, and the average Cq value of conidia collected at 72 h was considered the experimental ...
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Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus
... solution was sterilized separately and added aseptically to the culture medium. The pH was adjusted by adding orthophosphoric acid. Temperature was controlled at 30 0 C and the agitation rate was 300 rpm. The ...
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Improvement of submerged culture conditions to produce colorants by Penicillium purpurogenum
... The results obtained from the factorial designs, which analyzed the independent variables incubation period, or- bital stirring speed, pH, temperature, sucrose and yeast ex- tract concentrations under the production of ...
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MATERIALS AND METHODS Organism, maintenance and culture conditions
... All the experiments were carried out in triplicate and the results were analyzed by single linear regression analysis. The enzyme production by microorganisms is remarkably influenced by conditions like pH, ...
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MATERIAL AND METHODS Microorganism and culture conditions
... (Microorganism Culture Collection, Catholic University of Pernambuco, Recife, Brazil) isolated from mangrove sediment (Rio Formoso, Pernambuco, Brazil) was assayed for chitosan ...
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MATERIALS AND METHODS Microorganism and culture conditions
... conditions and heated at 100ºC for 15 minutes to inactivate the enzymes that could be part of their formulation. The detergents were added to the reaction mixture and the reaction was carried out under standard ...
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MATERIALS AND METHODS Organism and culture conditions
... Different strains of Thermoascus aurantiacus had been said to be good producers of endoglucanase and/or xylanases (8,9,13- 16,22,24-26). Many of these reports were in SmF and/or using optimized nutrient and fermentation ...
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