DNA extraction
DNA extraction from hair shafts of wild Brazilian felids and canids
... however, DNA must be extracted from ...shaft DNA from eight wild Brazilian felids, ocelot, margay, oncilla, Geoffroy’s cat, pampas cat, jaguarundi, puma, and jaguar, as well as the domestic cat and from ...
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Streamlining DNA barcoding protocols: automated DNA extraction and a new cox1 primer in arachnid systematics.
... for extraction of genomic DNA using the MagMAX Express Magnetic Particle Processor, an automated high throughput DNA extraction ...MagMAX DNA Multi-Sample ...
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CTAB methods for DNA extraction of sweetpotato for microsatellite analysis
... practical DNA extraction methods to ensure good quality and quantity DNA for these studies are yet to be ...for DNA extraction of six sweetpotato landraces using the CTAB ...
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Comparison of Different Methodologies for DNA Extraction from Aegla longirostri
... of DNA extraction from ...of DNA on agarose gel; nevertheless it was enough for the CO1 gene amplification by PCR ...allowed DNA extraction of better ...extracted DNA of ...
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COMPARISON OF COMMERCIAL DNA KITS AND TRADITIONAL DNA EXTRACTION PROCEDURE IN PCR DETECTION OF PORK IN DRY/FERMENTED SAUSAGES
... available dNa extraction kits (Wizard® Genomic dNa Purification Kit, High Pure PCr Template Kit, dNeasy mericon Food and GenejeT PCr Purification Kit), as well as standard phenol/chloroform iso- ...
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Direct RAPD Evaluation of Bacteria without Conventional DNA Extraction
... successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA ...template DNA was obtained without conventional DNA ...phenol ...
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An improved method for genomic DNA extraction from strawberry leaves
... Several extraction methods of genomic DNA for identification and characterization of genetic diversity in different plant species are routinely applied during molecular ...quality DNA in ...for ...
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Rapid Yeast DNA Extraction By Boiling And Freeze- Thawing Without Using Chemical Reagents And DNA Purification
... of DNA went out the cells either during the PCR cycles or during the centrifugation ...amplified DNA and did not interfere with subsequent PCR ...yeast DNA extraction were reliable and ...
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Comparação de protocolos de extração de DNA genômico de Capsicum spp. / Comparison of genomic DNA extraction protocols of Capsicum spp.
... DNA extraction. The present study aimed to compare protocols for plant DNA extraction for Capsicum ...Four extraction methods were compared: P1 Cetyltrimethylammonium bromide detergent ...
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Comparison of nine DNA extraction methods for the diagnosis of bovine tuberculosis by real time PCR
... All samples were derived from cattle carcasses in slaughterhouses inspected by the Serviço de Inspeção Federal (SIF) and sent to the official diagnosis facility in Brazil. Tissues contained granulomatous lesions ...
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MATERIALS AND METHODS Bacterial strains, growth conditions and DNA extraction
... and DNA extraction The rhizobial strains used in this study were the reference strain Bradyrhizobium japonicum USDA 110, and the crop inoculant strain Bradyrhizobium elkanii SEMIA 587; both strains were ...
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2) Antimicrobial susceptibility testing and DNA extraction of isolated strains
... total DNA extraction, applying the phenol-chloroform (1:1) method, according to the protocol described by Sambrook et ...Total DNA was read in agarose gel at ...
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Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria -assessment of DNA extraction methods and field applicability
... parasite DNA from RDTs was first shown by Veron et ...of DNA extraction from RDTs have been published [3,4] suggesting that RDTs are a reliable source for parasite DNA ...published DNA ...
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Evaluation of five methods for total DNA extraction from western corn rootworm beetles.
... extracted DNA depend greatly on the quality of the starting ...extract DNA from a variety of preserved specimens including alcohol-preserved samples or air-dried museum ...extract DNA from ...
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DNA extraction, PCR, and DNA sequencing
... o DNA Barcode em novas áreas para estabelecer novos registros de Liriomyza sativae no Brasil e também a determinação da diversidade genética intra e interpopulacional, e reconstruir a filogenia das espécies ...
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Sample collection and DNA extraction
... mitochondrial DNA control region (mtDNA) from various breeds of short and fat- tailed European sheep, Tapio & Grigaliunaite (2002) observed a higher level ...
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DNA extraction and molecular marker detection
... From these, plants carrying resistant alleles of the three target resistance genes (based on their markers genotyped) and which were phenotypically similar to the recurrent parents w[r] ...
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DNA extraction and SSR genotyping
... SSRs were amplified using Multiplex PCR Kit (Qiagen). PCR reactions, with a final volume of 5 uL, contained 1.0 ng of DNA, 2.5 uL of 2x Qiagen multiplex PCR Master mix (HotStar Taq DNA Polymerase, PCR ...
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Genetic materials and DNA extraction
... the DNA molecular technique is the best and able to detect differences at the genome ...Polymorphic DNA (RAPD) and Amplified Fragment Length Polymorphism (AFLP) are more frequently used for genetic ...
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Bacterial isolates and DNA extraction
... Amplification of an intragenic nodA gene fragment using a NodAforB and NodArevB primer set can exhibit positive results, with sizes ranging from 245 to 800 bp (Klonowska et al., 2012; Taulé et al., 2012). So, the ...
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