Expression vector
pSiM24 is a novel versatile gene expression vector for transient assays as well as stable expression of foreign genes in plants.
... Ti vector pSiM24 for plant transformation with maximum ...pSiM24 vector, the size of the backbone of the early binary vector pKYLXM24 (GenBank Accession ...binary vector pSiM24 is composed of ...
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Foxp3 represses retroviral transcription by targeting both NF-kappaB and CREB pathways.
... CREB expression vector resulted in nearly complete inhibition of Tax-mediated activation of the HTLV-I LTR, while blocking NF-jB activation by addition of a dominant-negative IKKb expression ...
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A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs.
... optimal expression. That the single G in the U6T7G-C24 vector sup- ports transcription by T7 polymerase but produced a lower absolute amount of RNA tends to support this notion (lane 4, Fig 3 and Table ...
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Qun Luo, Ying He, Deng-Yong Hou, Jian-Guo Zhang, Xian-Rong Shen
... To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was con- structed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression ...
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A glutathione s-transferase confers herbicide tolerance in rice
... Plant expression vector was introduced to Agrobacterium tumefaciens AGL0, and trans- formed into Oryza sativa ...over-expression vector, whereas they were not found in non-transformed plants ...
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Meilan Xue, Yinlin Ge, Jinyu Zhang, Qing Wang and Lin Hou
... its expression can exert a significant effect on cell fatty acid ...eukaryotic expression vector pEGFP-C3-n-3 and to express the n-3 Fad gene in human breast cancer cells (MCF-7 ...recombinant ...
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Estratégias para integração múltipla de cassetes de expressão no genoma de Komagataella phaffii
... of expression by providing extra-copies of a heterologous ...an expression vector carrying a drug-resistance marker following a screening for multicopy clones on plates with increasingly higher ...
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Cloning and expression of NS3 helicase fragment of hepatitis C virus and the study of its immunoreactivity in HCV infected patients
... The expression vector (pET102/D-TOPO), Ni- NTA purification system, anti-His Tag antibody, and HRP-conjugated anti human against IgG were purchased (Invitrogen, Frankfurt, ...
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Licenciada em Química Aplicada
... 43 AuNPs functionalized with nuclear localization signal (NLS) peptide, which are capable to bind specifically to nuclear membrane and must have facilitated a higher amount of AuNPs entry into the nucleus (Kang et al. ...
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EXPRESSION OF MOUSE BETA DEFENSIN 2 IN ESCHERICHIA COLI AND ITS BROAD-SPECTRUM ANTIMICROBIAL ACTIVITY Tianxiang Gong
... 2. Expression of fusion protein TrxA-mBD2 in ...the expression vector pET32a-mBD2, which was transformed into ...for expression fusion ...
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Lu Zheng, Ping Liang, JianBo Zhou, XiaoBing Huang, Yu Wen, Zheng Wang and Jing Li
... linear vector, the pMD18-T simple vector was used for direct cloning (T-A cloning) of PCR products, permitting blue/white screening since it was free of LacZ gene MCS but kept β-galactosidase ...eukaryotic ...
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High level constitutive expression of luciferase reporter by lsd90 promoter in fission yeast.
... new expression vector was constructed by cloning the putative promoter region of the lsd90 gene (earlier reported to be strongly induced by heat stress) into a previously reported high copy number ...
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IMMUNE RESPONSE TO DNA VACCINE EXPRESSING TRANSFERRIN BINDING PROTEIN A GENE OF PASTEURELLA MULTOCIDA Satparkash Singh
... mammalian expression vector in such a way that the former was transcribed under cytomegalovirus promoter whereas the latter was under IRES (internal ribosome entry site) promoter from encephalomyocarditis ...
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Gateway vectors for efficient artificial gene assembly in vitro and expression in yeast Saccharomyces cerevisiae.
... Recent progress in synthetic biology has made it possible to engineer gene regulatory networks with predictable behaviors [1]. Construction of de novo gene networks requires efficient assembly of DNA sequences including ...
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Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
... of expression by providing extra-copies of a heterologous ...an expression vector carrying a drug-resistance marker following a screening for multicopy clones on plates with increasingly higher ...
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Bao Zhen Feng, Peiqian Li
... acterized from Phytophthora capsici for the first time. The 1683 bp full-length cDNA of pclac2 en- coded a mature laccase protein containing 560 amino acids preceded by a signal peptide of 23 amino acids. The deduced ...
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A glutathione s-transferase confers herbicide tolerance in rice
... Plant expression vector was introduced to Agrobacterium tumefaciens AGL0, and trans- formed into Oryza sativa ...over-expression vector, whereas they were not found in non-transformed plants ...
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Rev. Bras. Psiquiatr. vol.35 suppl.2
... myc vector with tetracycline-regulated v-myc expres- sion. 26 This vector also contains a tetracycline transacti- vator that enhances the expression of v-myc from a minimal promoter from human ...
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Rabies virus vector transgene expression level and cytotoxicity improvement induced by deletion of glycoprotein gene.
... RV vector other than its infection properties such as the transgene expression level ...G expression; (2) the presumed increase of the L gene ...transgene expression level and the reduction of ...
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A Versatile Vector for In Vivo Monitoring of Type I Interferon Induction and Signaling.
... Although several plasmids containing ISRE-driven reporter elements have been generated, few studies have undertaken the task of improving such reporter plasmids and analyzing their pos- sible use for the in vivo ...
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