gel electrophoresis
Application of urea-agarose gel electrophoresis to select non-redundant 16S rRNAs for taxonomic studies: palladium(II) removal bacteria
... The 16S rRNA gene has been the most commonly used sequence to characterize bacterial communities. The classical approach to obtain gene sequences to study bacterial diversity implies: cloning amplicons, selecting clones ...
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An alkaline single-cell gel electrophoresis (comet) assay for environmental biomonitoring with native rodents
... single-cell gel electrophoresis (SCG) assay, a new procedure for evaluating DNA lesions (single strand breaks and alkali-labile sites), involves application of an electrical current to cells, which results ...
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New approach for segmentation and quantification of two-dimensional gel electrophoresis images
... two-dimensional gel electrophoresis images (2-DE) is a very complex task and current approaches addressing this problem still suffer from significant ...three gel sections are used to test the ...
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Protein profile analysis of Malaysian snake venoms by two-dimensional gel electrophoresis
... Abstract: Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques. In the present study, venoms ...
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Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis
... Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using ...
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Comparative Two-Dimensional Gel Electrophoresis Maps for Promastigotes of Leishmania amazonensis and Leishmania major
... two-dimensional gel electrophoresis (2-DE) separations to determine protein-expression profiles between highly infectious forms of the parasitic species ...infections, gel electrophoresis, ...
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Caracterização de cepas de referência de Leptospira sp utilizando a técnica de pulsed field gel electrophoresis.
... 19. Ribeiro RL, Machry L, Brazil JMV, Ramos TMV, Avelar KES, Pereira MM. Technical improvement to prevent DNA degradation of Leptospira spp in pulsed ield gel electrophoresis. Leters in Applied Microbiol ...
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Typing of Enterococcus faecium by polymerase chain reaction and pulsed field gel electrophoresis
... Polymerase chain reaction (PCR) with JB1 or REP consensus oligo- nucleotides and pulsed field gel electrophoresis (PFGE) were used to study genomic DNA extracted from 31 strains of enterococci. Eleven ATCC ...
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Multilocus Sequence Typing Scheme versus Pulsed-Field Gel Electrophoresis for Typing Mycobacterium abscessus Isolates
... Outbreaks of infections by rapidly growing mycobacteria following invasive procedures, such as ophthalmological, laparoscopic, arthroscopic, plastic, and cardiac surgeries, mesotherapy, and vaccination, have been ...
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Associated Risk Factors and Pulsed Field Gel Electrophoresis of Nasal Isolates of Staphylococcus aureus from Medical Students in a Tertiary Hospital in Lagos, Nigeria
... field gel patterns of the strains, nasal swabs were collected from 185 medical students in a teaching hospital in Lagos, ...Field Gel Electrophoresis (PFGE) was ...
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Molecular analysis of salmonella anteritidis isolates from the Caribbean by pulsed-field gel electrophoresis
... pulsed-field gel electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmo- nella enteritidis isolates from gastroenteritis cases in four Caribbean countries: Barbados, Saint Kitts and Nevis, Saint ...
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Separation of Proteins using Gel Electrophoresis
... ABSTRACT: Red scorpion (Mesobuthus tamulus or Buthus tamulus) venom samples were collected at different regions of India: western (Chiplun and Ahmednagar from Maharashtra State) and southern (Ratnagiri and Chennai from ...
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Alkaline Gel Electrophoresis Assay to Detect DNA Strand Breaks and Repair Mechanisms in Escherichia coli
... Lesions leading to cell inactivation in prokaryotes could be, for instance, alkali-labile sites and strand breaks, which can be detected by alkaline sucrose gradient (MacGrath and Williams, 1966). This experimental ...
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Polyacrylamide gel electrophoresis-SDS as a tool to study myofibrillar proteins. A review.
... un gel vertical de agarosa, con esto las proteínas de pesos moleculares entre 200 y 4,0000 kDa pueden ser claramente separadas, este sistema es extensivamente utilizado en muestras de músculo (Greaser y Warren, ...
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Lectin isolated from Brazilian seeds of velvet bean ( Mucuna pruriens (L) DC.) presents analgesic, anti inflammatory and antihemolytic action
... agarose gel electrophoresis (PAGE), the hemolytic activity on human erythrocytes was determined, as well as its antinociceptive action by models of writhing induced by acetic acid and formalin, in addition ...
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Estudo do proteoma e imunoproteoma salivar do carrapato de bovinos, Rhipicephalus...
... Differential Gel Electrophoresis) para avaliar a expressão diferencial de proteínas do carrapato quando alimentado em diferentes hospedeiros, além de análises de imunoproteoma que empregam o sequenciamento ...
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Caracterização da diversidade de eucariotas fototróficos provenientes de águas ácidas de mina
... num gel de eletroforese em ...no gel, durante a eletroforese, são condicionados pelo seu tamanho e pela sua ...em gel com gradiente desnaturante (DGGE – “Denaturing Gradient Gel ...
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Higher isolation of NDM-1 producing Acinetobacter baumannii from the sewage of the hospitals in Beijing.
... Field Gel Electrophoresis (PFGE) was performed to determine the genetic relationship among the NDM-1 producing isolates from sewage and human, as well as the clinical strains without ...field gel ...
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Phenotyping and genotyping methods applied to investigate the relatedness of Brazilian isolates of Enterobacter cloacae
... In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a ...
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Impact of Amazon land use on the community of soil fungi
... ABSTRACT: Considered as one of the most biodiverse biomes, the Amazon has a featured role in the discovery of new species of plants, animals and microorganisms, which may be important for the functionality of different ...
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