Gel filtration
Gel filtration on Sephadex G-50
... Palamneus gravimanus crude venom was resolved into four bound and one unbound protein peak on a CM-Sephadex C-25 column (Figure 1). Fractions number 70-95, which showed maximum toxicity to white mice, were pooled, ...
21
Purification of an L-amino acid oxidase from Bungarus caeruleus (Indian krait) venom More SS (1), Kiran KM (2) Veena SM (2), Gadag JR (2)
... proteolytic enzymes, hyaluronidases and phosphodiesterases, which are well characterized. However, L-amino acid oxidase (LAO EC.1.4.3.2) from snake venoms has not been extensively studied. A novel L-amino acid oxidase ...
16
Production of membranes for filtration of biomolecules
... with gel-like characteristics ...the gel filtration ...functional gel filtration medium, there are a number of other requirements, such as adequate pore size distribution, rigidity, ...
93
Characterization of a β-glucanase produced by Rhizopus microsporus var. microsporus, and its potential for application in the brewing industry
... Results: The zygomycete microfungus Rhizopus microsporus var. microsporus secreted substantial amounts of β-glucanase in liquid culture medium containing 0.5% chitin. An active protein was isolated by gel ...
9
Purification of bovine pancreatic glucagon as a by-product of insulin production
... The isoelectric precipitate was purified by ion-exchange chromatography on Q- Sepharose FF in buffer containing urea, by gel filtration on Sephadex G-25, and by ion- exchange chromatography on S-Sepharose ...
6
Flooding tolerance and cell wall alterations in maize mesocotyl during hypoxia (1)
... The gel filtration on Sephadex G-200, from pec- tic substances, hemicellulose 2M and 4M fractions isolated from apical and basal mesocotyl segments with normal appearance, translucid, and constriction zone ...
9
Metallothioneins in the clam Ruditapes decussatus: an overview
... by gel filtration chroma- tography, differential pulse polarography and SDS-PAGE, after Cd exposure, revealed that MT is induced in different tissues (whole soft tis- sues, gills, digestive gland and ...
5
Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy.
... by gel filtration ...by gel filtration chromatography gave results consistent with similar analyses of isolated CMG complexes from Drosophila of an estimated size of about ...with gel ...
10
Estudo da estrutura e função das hemoglobinas isoladas das arraias da Amazônia do gênero Potamotrygon ()
... The hemolysates isolated from Amazonian stingrays, Potamotrygon sp. Gel filtration experiments of ferro and ferrihemoglobin show that all of the hemolysates are tetramers which do no[r] ...
12
Aminopeptidase de Mesorhizobium sp. descoberta por mineração de dados genômicos com aplicações biotecnológicas e seu impacto na fisiologia bacteriana
... ABSTRACT - The genomic data mining analysis of a Mesorhizobium sp. revealed the presence of a 1257-bp open reading frame containing the Mesoamp gene, which encodes a protein of 418 amino acids. The deduced amino acid ...
35
PURIFICATION AND PARTIAL CHARACTERIZATION OF A COAGULANT SERINE PROTEASE FROM THE VENOM OF THE IRANIAN SNAKE Agkistrodon halys
... by gel filtration on a Sephadex G-50 column, ion-exchange chromatography on a DEAE-Sepharose and high performance liquid chromatography (HPLC) on a C18 ...polyacrylamide gel electrophoresis ...
13
Surface Imprinting of Silica Gel by Methyldopa and Its Application in the Solid Phase Extraction Procedure Mohammad Taghi Vardini and Leila Mardani
... silica gel material was prepared by surface molecular imprinting technique combined with a sol-gel process on the supporter of activated silica gel and used as a solid-phase extraction for the ...
10
Purificação e caracterização parcial de tonina símile a partir de placenta de humano
... The existence of a renin-angiotensin system (RAS) in the female reproductive organ has been demonstrated. In this tissue, RAS could be acting independently or in accord with the circulating system, participating in the ...
107
Caracterização da diversidade de eucariotas fototróficos provenientes de águas ácidas de mina
... num gel de eletroforese em ...no gel, durante a eletroforese, são condicionados pelo seu tamanho e pela sua ...em gel com gradiente desnaturante (DGGE – “Denaturing Gradient Gel ...
133
Efficient filtration system for paraffin-catalyst slurry separation
... internal filtration is the most efficient tech- nique from an economic and environmental point of view ...a filtration or mag- netic ...but filtration techniques still retain their impor- tance ...
7
Prony filtration of seismic data: theoretical background
... Prony filtration with frequency 43Hz ...Prony filtration obtained for frequency of 54Hz it is possible to clearly distinguish four reflections, located between horizons C and ...
20
Desenho e validação de iniciadores microssatélites SSR para mamoneira.
... Agarose gel was used for detection of the ampliied products, and denaturing polyacrylamide gel for the optimization of PCR conditions and the identiication of ...
7
Secagem de gel ágar.
... Em nenhum momento verificou-se visualmente qualquer tipo de ruptura no gel devido ao seu encolhimento. Houve apenas o retorcimento das amostras quando apresentavam diâmetro entre 3 e 4.10 -2 m. A cor final de ...
125
Modificação da sílica propiletilenodiamina com formaldeído, estudo do equilíbrio e da cinética de adsorção dos íons cádmio, chumbo e cromato
... remover impurezas do meio ambiente (SILVA et al., 2007, AGUIAR et al., 2002), como, por exemplo, a remoção de metais pesados de leitos e efluentes industriais (AUGUSTO FILHA et al., 2007a, SOUSA et al., 2007). Tais ...
97