Gene knockout
Pol II-expressed shRNA knocks down Sod2 gene expression and causes phenotypes of the gene knockout in mice.
... target gene can be ...standard knockout technology reduces the target gene expression to 50% or 0% in most instances, transgenic RNAi can reduce the target gene to more variable degrees in ...
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Neuronal and endothelial nitric oxide synthase gene knockout mice
... eNOS gene knockout ...eNOS knockout mice is consistently 20-30% higher than that of wild-type animals, com- pared under identical conditions of anesthe- sia and in the awake state ...
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Unintentional miRNA ablation is a risk factor in gene knockout studies: a short report.
... In the mouse, stable disruption of a gene is typically accomplished using gene trap mutagenesis or targeted homologous recombination. We wish to communicate the overlooked possibility of unintentionally ...
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A Simplified Method for Gene Knockout and Direct Screening of Recombinant Clones for Application in Paenibacillus polymyxa.
... this gene knockout system, deletion mutations of ...-amylase gene, its structural gene flanking regions were cloned into a pGEM7Zderivative containing the cat gene which inactivates ...
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11β-hydroxysteroid dehydrogenase type 1 gene knockout attenuates atherosclerosis and in vivo foam cell formation in hyperlipidemic apoE⁻/⁻ mice.
... Figure S1 Targeted disruption of the 11bHSD1 gene locus. A) Targeting strategy used to disrupt the 11bHSD1 locus. Homologous recombination (represented by X) between the targeting vector and the 11bHSD1 ...
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Characterisation of iunH gene knockout strain from Mycobacterium tuberculosis
... reporter gene, and the sacB counterselectable ...using gene-specific screening primers forward (5’- ttcag- gaaacgagcgaaggt-3’) and reverse (5’-gtgctatccggcgga- cac-3’) to determine whether the WT or the KO ...
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Age-Dependent Defects of Regulatory B Cells in Wiskott-Aldrich Syndrome Gene Knockout Mice.
... Accordingly, while the concentration of IL-10 detected in the culture medium of stimulated CD1d high CD5 + Breg cells from younger WKO mice did not differ from WT control mice, IL- 10 pr[r] ...
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High-resolution profiling of stationary-phase survival reveals yeast longevity factors and their genetic interactions.
... interacting gene (right). (B) Micrographs of WT and gene-knockout strains expressing the GFP-Atg8 fusion protein under basal conditions (SD, left) and after 7 h of nitrogen starvation (SD-N, ...
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Differential Bees Flux Balance Analysis with OptKnock for in silico microbial strains optimization.
... yield. Bees Algorithm (BA) was first introduced by Pham et al. [9], which is a typical meta-heuristic optimization approach that has been applied to several problems, such as controller formation [10], image analysis ...
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Analysis of microsatellite polymorphism in inbred knockout mice.
... Previously, we found that the genotype of 42 out of 198 mouse microsatellite loci, which are distributed among all chromosomes except the Y chromosome, changed from monomorphism to polymorphism (CMP) in a genetically ...
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The Effect of Different Photoperiods in Circadian Rhythms of Per3 Knockout Mice
... clock gene knockout mice have shown alterations in the endoge- nous circadian period length, loss of persistence of circadian rhythms, and disturbed sleep-wake cycles [3, ...
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Model-driven redox pathway manipulation for improved isobutanol production in Bacillus subtilis complemented with experimental validation and metabolic profiling analysis.
... Bacterial strains, plasmids, and genetic manipulation The strains and plasmids used in this work are listed in Table 1. All B. subtilis mutants used in this work were the derivatives of B. subtilis 168 which had been ...
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β2-adrenergic receptor knockout mice exhibit A diabetic retinopathy phenotype.
... receptor knockout animals, but this did not achieve statistical significance versus that seen in wild type ...receptor knockout mice [10] and animals treated with streptozotocin to induce diabetes [6,7]; ...
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ÁCIDO LINOLEICO CONJUGADO E EXERCÍCIO FÍSICO: EFEITOS NA ATEROSCLEROSE DE CAMUNDONGOS APOE (-/-)
... O acompanhamento de indivíduos praticantes de exer- cícios físicos em academias tem demonstrado que a prática deste tem sido uma estratégia para prevenção ou reversão da doença arterial coronariana (DAC) e dislipidemias. ...
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TLR2, TLR4 and the MYD88 signaling pathway are crucial for neutrophil migration in acute kidney injury induced by sepsis.
... Total RNA was isolated using the Trizol Reagent (Invitrogen, Carlsbad, CA). First-strand cDNAs were synthesized using the MML-V reverse transcriptase kit (Promega, Madison, Wisconsin, USA). Reverse transcriptase ...
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Evolução da lesão aterosclerótica e perfil lipídico de camundongos knockout Apo E alimentados com resíduo de café seco e fermentado
... A cafeicultura é uma atividade agrícola que produz um grande volume de resíduos líquidos e sólidos, gerando custos com o tratamento e destinação dos mesmos. O resíduo do fruto do cafeeiro, por ser rico em macro e micro ...
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Unmet expectations: miR-34 plays no role in p53-mediated tumor suppression in vivo.
... genetic knockout mod- els of either miR-34a or miR-34b/c, or a compound mutant animal harboring ho- mozygous deletion of all three miR-34 family members (miR-34 TKO ) ...
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Resistência a hormônios tireóideos: contribuição da transgênese para a compreensão da síndrome.
... Os camundongos knockout para a isoforma b dos TRs apresentam, como fenótipo principal, surdez por anomalias severas no desenvolvimento da cóclea e resistência hipofisária à ação dos horm[r] ...
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Large-scale in silico mapping of complex quantitative traits in inbred mice.
... We have demonstrated the robustness and reproducibility of GWA analysis by comparing QTLs derived from GWA with those derived from previous linkage analyses in inbred mice. In a recent GWA analysis of lung tumor ...
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Caracterização fenotípica de camundongos knockout para neurolisina
... The oligopeptidase neurolysin (EC 3.4.24.16; Nln) was first identified in rat brain synaptic membranes and shown to ubiquitously participate in the catabolism of bioactive peptides such as neurotensin and bradykinin. ...
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