Gene transfer
Gene transfer utilizing pollen-tubes of Albuca
... seed setting varied between natural and treated pollen grains. However, setting was 24.8% for T. violacea and 74.7% for A. nelsonii in the selection treatment, signifying that Km-resistant pollen effected seed setting, ...
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A rice Stowaway MITE for gene transfer in yeast.
... in gene transfer, a rice Stowaway MITE previously shown to excise efficiently in yeast was engineered to carry cargo genes (neo and gfp) for delivery into the budding yeast ...cargo gene cassettes ...
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Perspectives for cancer immunotherapy mediated by p19Arf plus interferon-beta gene transfer
... functional gene sequences but greatly diverge with respect to transcriptional regulation ...our gene transfer ...our gene transfer ...
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Cancer progression mediated by horizontal gene transfer in an in vivo model.
... vertical gene transfer, but this paradigm ignores that DNA circulates in higher organisms and that it is biologically active upon its uptake by recipient ...
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The cobweb of life revealed by genome-scale estimates of horizontal gene transfer.
... horizontal transfer and incorporation of genetic ...horizontal gene transfer (HGT) affects the core genealogical history of organisms remains ...orthologous gene clusters, and we detect ...
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Evaluation of pEPI-1 and pEPito expression systems for gene transfer to the retina
... In this study, we evaluated the ability of pEPI-vector and its derivative pEPito as an expression system for gene transfer to the retina. These plasmids contain in their backbone a S/MARs. Previous studies ...
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An adenovirus vector incorporating carbohydrate binding domains utilizes glycans for gene transfer.
... performed gene transfer assays in two cell lines with markedly different levels of CAR expression: CAR-deficient Chinese hamster ovary (CHO) cells and a CHO-derived cell line, CHO- hCAR, which stably ...
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Gene transfer and genome-wide insertional mutagenesis by retroviral transduction in fish stem cells.
... for gene transfer and integration in dividing cells of diverse or- ...mediated gene transfer and genome-wide IM in fish stem cells from medaka and ...upon gene trapping (GT) via ...
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Gene transfer to primary corneal epithelial cells with an integrating lentiviral vector
... confluency) prior to transduction. Gene transfer was monitored using fluores- cence microscopy and fluorescence activated cell sorter (FACS). We evaluated the transduction efficiency (TE) over time and the ...
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Leu452His mutation in lipoprotein lipase gene transfer associated with hypertriglyceridemia in mice in vivo.
... hydrodynamics-based gene delivery (HD). Mutated-LPL (MLPL) gene transfer significantly increased the concentrations of plasma MLPL and triglyceride (TG) but significantly decreased the activity of ...
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Functional biogeography as evidence of gene transfer in hypersaline microbial communities.
... functional gene groups ...of gene transfer between informational genes (16S) and operational genes (func- tion) ...of gene transfer is dependent on whether the function is part of the ...
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AAV-mediated gene transfer of the obesity-associated gene Etv5 in rat midbrain does not affect energy balance or motivated behavior.
... mediated gene transfer of Etv5 into the VTA/SNpc of rats did not alter expression of tyrosine hydroxylase in ...AAV-mediated gene transfer of Etv5 in the VTA/SNpc did not affect measures of ...
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Horizontal Gene Transfer and its Detection
... horizontal transfer datasets if the analysis considers each gene as a data ...a gene has a fixed number of variables associated with it, where such variables are indicators of phylogenetic or ...
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Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts
... a gene coding for a green fluorescent protein (GFP) (Chalfie et ...transient gene expression has been reported under electric field strengths causing more than 50% reduction in protoplast vi- ability (Fromm ...
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Characterization of an ancient lepidopteran lateral gene transfer.
... GH31-like gene called HMEL002892 with strong BLAST similarity to DpGH31 in the recently published genome of Heliconius ...melpomene gene is also related to Figure ...
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A biolistic process for in vitro gene transfer into chicken embryos
... DNA transfer to chicken embryos culti- vated in culture medium was successful. The CMV promoter used caused strong expres- sion in all embryo and extra-embryonic tis- sues, and the lacZ and GFP reporter genes were ...
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Adeno-associated virus for cystic fibrosis gene therapy
... the gene transfer of AAV vectors for CF gene therapy, the activities of different promoters, cytomegalovirus (CMV), Rous sarcoma virus and cytomega- lovirus promoter conjugated/β - actin (CB), were ...
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Modulation of the expression of the transcription factor Max in rat retinal ganglion cells by a recombinant adeno-associated viral vector
... (8). Gene transfer vectors based on AAV combine a number of advantages: AAV is naturally defective for replication and is considered nonpathogenic ...cells, transfer a gene to a number of cell ...
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Rev. Bras. Hematol. Hemoter. vol.22 número3
... first gene transfer protocol which entered clinical practice involved gene marking ...influencing gene transfer efficiency, and the mechanism of relapse in patients receiving stem cell ...
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MOLECULAR BIOLOGY OF MOLLICUTES
... conjugative transfer, and integration with the host (Burrus and Waldor ...mass transfer of genes is not believed to have had a very signifi cant role in the evolution of ...which gene transfer ...
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