Guanylyl cyclase
Differential contribution of the guanylyl cyclase-cyclic GMP-protein kinase g pathway to the proliferation of neural stem cells stimulated by nitric oxide
... enzyme guanylyl cyclase. Guanylyl cyclase cata- lyzes the conversion of GTP to cyclic GMP (cGMP), which in turn can act on further downstream targets such as the cGMP-dependent kinase (PKG) ...
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Mapping Soluble Guanylyl Cyclase and Protein Disulfide Isomerase Regions of Interaction.
... Soluble guanylyl cyclase (sGC) is a heterodimeric nitric oxide (NO) receptor that produces cyclic GMP. This signaling mechanism is a key component in the cardiovascular system. NO binds to heme in the β ...
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Loss of guanylyl cyclase C (GCC) signaling leads to dysfunctional intestinal barrier.
... Guanylyl cyclase C (GCC) is a transmembrane receptor for the endogenous peptides guanylin (GN) and uroguanylin (UGN) and for bacterial heat stable enterotoxin (ST) ...
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Nitroxyl (HNO) stimulates soluble guanylyl cyclase to suppress cardiomyocyte hypertrophy and superoxide generation.
... Similar to Angeli’s salt, the hypertrophic response to Ang II in neonatal rat cardiomyocytes is also prevented by the natriuretic peptide and particulate guanylyl cyclase (pGC) ligand, BNP (1 m mol/L). BNP ...
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UV-B Filter Octylmethoxycinnamate Induces Vasorelaxation by Ca2+ Channel Inhibition and Guanylyl Cyclase Activation in Human Umbilical Arteries
... soluble guanylyl cyclase (sGC) that increases the cyclic guanosine monophosphate (cGMP) intracellular levels and an inhibition of L-type voltage-operated Ca 2+ channels (L-Type ...
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Identification of residues in the heme domain of soluble guanylyl cyclase that are important for basal and stimulated catalytic activity.
... Nitric oxide signals through activation of soluble guanylyl cyclase (sGC), a heme-containing heterodimer. NO binds to the heme domain located in the N-terminal part of the b subunit of sGC resulting in ...
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Nucleotidyl cyclase activity of particulate guanylyl cyclase A: comparison with particulate guanylyl cyclases E and F, soluble guanylyl cyclase and bacterial adenylyl cyclases CyaA and edema factor.
... Guanosine 3 9,59-cyclic monophosphate (cGMP) is a well- established second messenger that is involved in the regulation of many physiological processes like blood homeostasis, neuro- transmission, intestinal secretion, ...
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Vasodilator e ffects and putative guanylyl cyclase stimulation by 2-nitro-1- phenylethanone and 2-nitro-2-phenyl-propane-1,3-diol on rat aorta
... guanylate cyclase (sGC) pathway that increases the levels of cyclic guanosine 3',5'- monophosphate (cGMP), a second messenger with relaxant properties on smooth muscle cells (Ignarro et ...
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Hydrogen peroxide alters splicing of soluble guanylyl cyclase and selectively modulates expression of splicing regulators in human cancer cells.
... In addition, we show that the H 2 O 2 treatment of MDA-MD- 468 cells selectively decreases protein levels of PTBP1 and hnRNP A2/B1 splice factors identified as potential a 1 gene splicin[r] ...
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effect of 1nitro2phenylethane in mice pancreatic acinar cells subjected to taurocholate : putative role of guanylyl cyclasederived 8nitrocyclicGMP
... The nitroderivative 1-nitro-2-phenylethane (NPE) was recently described as a compound possessing heme-dependent soluble guanylyl cyclase (sGC) stimulating properties in vascular smooth muscle cells. In this ...
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Adenylyl cyclase types I and VI but not II and V are selectively inhibited by nitric oxide
... Adenylyl cyclase (AC) isoforms catalyze the synthesis of 3',5'-cyclic AMP from ...enzyme guanylyl cyclase, but little has been reported on the effects of NO on other important second messengers, such ...
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Mutational analysis gives insight into substrate preferences of a nucleotidyl cyclase from Mycobacterium avium.
... Adenylyl cyclase assays were carried out with approximately 500 nM of protein (50 mM MES, HEPES and diethanolamine - a triple buffer system, at appropriate pH), 10 mM NaCl, 5 mM b- mercaptoethanol, 1 mM ATP, 11 mM ...
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Role of soluble guanylate cyclase activation in the effect of the HO1CO pathway against alendronateinduced gastricdamage in rats
... Our objective was to evaluate the role of soluble guanylate cyclase (sGC) activation in the gastro- protective effect of the HO-1/CO pathway against alendronate-induced gastric damage in rats. Rats were ...
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Ca2+ influx and tyrosine kinases trigger Bordetella adenylate cyclase toxin (ACT) endocytosis. Cell physiology and expression of the CD11b/CD18 integrin major determinants of the entry route.
... adenylate cyclase toxin (ACT) which enters human phagocytes and catalyzes the unregulated formation of cAMP, hampering important bactericidal functions of these immune cells that eventually cause cell death by ...
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Pituitary adenylate cyclase activating peptide deficient mice exhibit impaired thymic and extrathymic regulatory T cell proliferation during EAE.
... It has been shown that the nervous system and the immune system interact with each other during health and disease. In this regard, there is robust evidence that neurological mediators, including multiple ...
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Bordetella pertussis commits human dendritic cells to promote a Th1/Th17 response through the activity of adenylate cyclase toxin and MAPK-pathways.
... The complex pathology of B. pertussis infection is due to multiple virulence factors having disparate effects on different cell types. We focused our investigation on the ability of B. pertussis to modulate host ...
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Closure of the human TKFC active site: comparison of the apoenzyme and the complexes formed with either triokinase or FMN cyclase substrates
... The main purpose of this study was to look for further confirmation of the trend towards active- site closure and to analyze whether substrate binding could influence protein domain mobility. In particular, we wondered ...
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Calpain-Mediated Processing of Adenylate Cyclase Toxin Generates a Cytosolic Soluble Catalytically Active N-Terminal Domain.
... The peptides were separated from the uncleaved protein by molecular exclusion chromatography in a Superdex 200 10/ 300 GL High performance column (Tricorn Superdex) and their catalytic activity assayed as described under ...
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Tracing the origins of the Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP)
... adenylate cyclase activating polypeptide is proposed to be one of the most well-conserved neuropeptides in the animal kingdom since it has been reported to exist from invertebrates to ...
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Bifunctional Homodimeric Triokinase/FMN Cyclase
... FMN cyclase activities in BL21 cells transformed with plasmid pGEX-6P-3-hF2 that encodes a GST-human DHA kinase/FMN cyclase fusion protein ...kinase/FMN cyclase plus an N-terminal extension of 11 ...
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