High-Throughput Screening Assays
Use of activity-based probes to develop high throughput screening assays that can be performed in complex cell extracts.
... is high throughput screening ...HTS assays have traditionally been performed with either a recombinant form of the target enzyme or with purified native enzyme ...extract-based assays ...
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A screen against Leishmania intracellular amastigotes: comparison to a promastigote screen and identification of a host cell-specific hit.
... cell-based screening assay using a human macrophage cell line infected with ...enables screening of compounds directly against the intracellular stage of the ...this screening method with traditional ...
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High-throughput screening assay used in pharmacognosy: Selection, optimization and validation of methods of enzymatic inhibition by UV-visible spectrophotometry
... In research laboratories of both organic synthesis and extraction of natural products, every day a lot of products that can potentially introduce some biological activity are obtained. Therefore it is necessary to have ...
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High throughput screening for anti-Trypanosoma cruzi drug discovery.
... HTS assays based on recombinant reporter parasites An increasing knowledge of ...the screening of large compound ...inhibitory assays, these genetically engineered microorganisms were shown to ...
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TRAIL-Based High Throughput Screening Reveals a Link between TRAIL-Mediated Apoptosis and Glutathione Reductase, a Key Component of Oxidative Stress Response.
... The potent and safe to primary hepatocytes combined treatments of NSC130362 and oxida- tive stress inducers against a variety of cancer cells as well as the effect of NSC130362 on the TRAIL pathway undoubtedly warranted ...
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Nanoscale Synaptic Membrane Mimetic Allows Unbiased High Throughput Screen That Targets Binding Sites for Alzheimer's-Associated Aβ Oligomers.
... 6. Screening strategy effectively eliminates false positives. (a) Screening assays used to evaluate the effect of Spectrum Collection molecules on AβO ...counterscreening assays designed to ...
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Use of an in vivo FTA assay to assess the magnitude, functional avidity and epitope variant cross-reactivity of T cell responses following HIV-1 recombinant poxvirus vaccination.
... of high functional avidity and have a high capacity to recognize HIV epitope variants, when compared to HIV-1 infection ...vaccines, assays that can effectively measure CTL quality specifically in ...
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High-content, high-throughput screening for the identification of cytotoxic compounds based on cell morphology and cell proliferation markers.
... such assays are routinely used for in vitro toxicity testing as considerable overlap occurs between the toxicity of hepatotoxic, nephrotoxic and cardiotoxic com- pounds in the cell lines commonly used to assess ...
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A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of Cell Lysis.
... Mycobacterium tuberculosis (Mtb) is well-established to be one of the most important bac- terial pathogens for which new antimicrobial therapies are needed. Herein, we describe the development of a high ...
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Tackling heterogeneity: a leaf disc-based assay for the high-throughput screening of transient gene expression in tobacco.
... Nicotiana tabacum is a well-characterized platform for the production of a wide range of recombinant proteins, such as antibodies, vaccines and biopharmaceuticals [1]. Although plant- based production platforms have many ...
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A Sensitive and Robust High-Throughput Screening Assay for Inhibitors of the Chikungunya Virus nsP1 Capping Enzyme.
... Failure to determine ideal assay parameters and assess assay stability during initial develop- ment stages could result in the identification of false hits, inability to detect active compounds or simply the lack of a ...
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A microfluidic platform for high-throughput multiplexed protein quantitation.
... and high-dynamic range with ...*100,000 assays using our ...by screening all 64 combinations of 4 primary biotinylated anti-PSA mouse monoclonal antibodies, 4 secondary anti-PSA rabbit poly- and ...
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High-throughput screening reveals alsterpaullone, 2-cyanoethyl as a potent p27Kip1 transcriptional inhibitor.
... luciferase-based assays for transcriptional screening has been around for over a decade, but to our knowledge, using this method to screen for small molecules that transcriptionally repress CDK inhibitors ...
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Identification of fluorescent compounds with non-specific binding property via high throughput live cell microscopy.
... for screening for compounds, with a non-stickiness ...for high-throughput imaging, can identify hits that can later be tested in three dimensional plant species at a lower throughput, ...new ...
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Cheburator software for automatically calculating drug inhibitory concentrations from in vitro screening assays.
... vitro screening of natural and synthetic compounds is an important first stage in the selection of new anticancer drugs throughout the ...earlier screening programs based on in vivo mouse leukemia ...vitro ...
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Screening for novel LRRK2 inhibitors using a high-throughput TR-FRET cellular assay for LRRK2 Ser935 phosphorylation.
... a high-throughput screening (HTS) compatible Time- Resolved Fo¨rster Resonance Energy Transfer (TR-FRET) assay using either LRRKtide or Nictide as the substrate [5,23,24], standard radioactive ...
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An assay suitable for high throughput screening of anti-influenza drugs.
... The ACA-less gene for M2 (from residue 2–49) was synthesized with flanked NdeI and BamHI restrictions sites in pUC57 by Genscript. pColdII m2 was constructed by transferring M2 gene to pColdII(sp-4) with the former two ...
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Rapid identification of antifungal compounds against Exserohilum rostratum using high throughput drug repurposing screens.
... colorimetric assays using 2,3-bis(2- methoxy-4-nitro-5-[(sulphenylamino)carbonyl]-2H-tetrazolium- hydroxide (XTT) and a fluorometric assay using AlarmaBlue have been developed ...in high-throughput ...
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WormScan: a technique for high-throughput phenotypic analysis of Caenorhabditis elegans.
... are high-throughput assessment of a greater range of phenotypic endpoints including determination of mortality and quantification of the mobility of ...
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Achievement of constitutive fluorescent pLEXSY-egfp
... The assays were done using different number of Leishmania per well, as described in #cell/well. The data indicate that GFP fluo- rescence assay is more sensible than Resazurin because similar % of live cells were ...
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