Nuclear transfer
Chemically Assisted Enucleation Results in Higher G6PD Expression in Early Bovine Female Embryos Obtained by Somatic Cell Nuclear Transfer
... cell nuclear transfer ...improve nuclear reprogramming in nuclear transfer (NT)–derived embryos because it results in lower damage and higher cytoplasm content than conventional ...as ...
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Bovine conceptus of Bos indicus produced by somatic cell nuclear transfer and parthenogenesis present morphological variations since the blastocyst stage
... In cattle, embryo development is characterized by the appearance of two distinct cell layers, the trophectoderm and the inner cell mass. The latter will undergo differentiation to form the embryonic disc consisting of ...
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Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte
... and offspring: A total of eight reconstructed blastocysts were transferred to two recipient heifers to generate gestations. One recipient was slaughtered at 55 days of gestation to obtain fetal tissue for analysis, ...
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Isolation and characterization of mesenchymal stem cells derived from bovine Wharton’s jelly and their potential for use in cloning by nuclear transfer
... The prevention of epigenetic errors has been suggested as a key to improve the success rate of animal cloning (WANG et al., 2011). Studies have shown significant differences between cell lines in the ability to produce ...
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Scriptaid Treatment Decreases DNA Methyltransferase 1 Expression by Induction of MicroRNA-152 Expression in Porcine Somatic Cell Nuclear Transfer Embryos.
... cell nuclear transfer (SCNT) is thought to be the main cause of low cloning ...the nuclear reprogramming mechanisms underlying the effects of Scriptaid on the improve- ment of porcine SCNT ...
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The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells
... 2008). Nuclear transfer from a somatic cell to an enu- cleated oocyte has been used to generate nuclear transfer embryonic stem cells (ntESCs) (Gurdon and Wilmut, ...
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Uncoupled embryonic and extra-embryonic tissues compromise blastocyst development after somatic cell nuclear transfer.
... cell nuclear transfer (SCNT) is the most efficient cell reprogramming technique available, especially when working with bovine ...embryo transfer at Day 7, elongation and gastrulation defects were ...
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Day-3 Medium Changes can Affect Developmental Potential of Porcine Somatic Cell Nuclear Transfer and Parthenogenesis Embryos In Vitro
... cell nuclear transfer (SCNT) embryos after day-3 medium change with fresh embryo culture medium to that of embryos that did not have a medium change (monoculture ...
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SOMATIC CELL NUCLEAR TRANSFER AS THE METHOD OF CHOICE FOR THE PRODUCTION OF GENETICALLY MODIFIED ANIMALS
... cell nuclear transfer (SCNT) enabled the generation of genetically modified animals utilizing previously modified and selected cell lineages as nuclei donors, assuring therefore the generation of ...
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Handmade cloned transgenic sheep rich in omega-3 Fatty acids.
... Genotype analysis using 13 ovine microsatellite markers demonstrated that all 3 transgenic sheep shared the same genotype to that of the nuclear donor cells (Table 4). The presence and function of mfat-1 gene in ...
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Chromatin modifying agents in the in vitro production of bovine embryos
... in nuclear transfer in pigs: valproic acid, which resulted in higher levels of Oct4 expression [105] and scriptaid [94] and sodium butyrate [106], which resulted in cloned embryos with acetylation levels ...
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THE EVOLVING PICTURE IN OBTAINING GENETICALLY MODIFIED LIVESTOCK
... Animal cloning by somatic cell nuclear transfer (SCNT) gave another perspective on the production of genetically modified livestock (Figure 3B). Shortly after these initial reports on cloned mammals (WILMUT ...
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Highly efficient generation of GGTA1 biallelic knockout inbred mini-pigs with TALENs.
... for nuclear transfer, combining with transcription activator-like effector nucleases (TALENs) and successfully generated α-1,3-galactosyltransferase (GGTA1) gene biallelic knockout (KO) ...as nuclear ...
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O medo nuclear
... energia nuclear se estabeleceu na sociedade desde que foi apresentada à humanidade pelos holocaustos de Hiroshima e Nagasaki em 1945, sob a forma do que se poderia chamar “o pior caso de marketing da ...
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An economic road to peace, a peaceful road for growth: regional integration through the side door in Western Europe and South America
... for nuclear energy development had increased mistrust between neighbors and moreover, had raised suspicions from the international community regarding their motives in pursuing nuclear ...The nuclear ...
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Phase transfer catalysis.
... PHASE TRANSFER CATALYSIS - Since its discovery, phase transfer catalysis (PTC) has grown considerably and nowadays is one of the most versatile preparative ...
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Cold nuclear fusion reactor and nuclear fusion rocket
... Please note that the patent is expected to be completely resolved once and for all mankind faces energy and environmental protection problem, so its rid progress each delayed day, all mankind will have to take more than ...
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The role of the state in development promotion: nuclear technology as a path to national development and autonomy
... the nuclear issue came to acquire relevance within the political agenda; and (3) to the analysis of the rereading of the development project by the Workers’ ...the nuclear issue under the Workers’ Party ...
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Braz. J. Phys. vol.34 número3A
... on Nuclear Physics and the XXVI Workshop on Nuclear Physics in Brazil took place, as a joint and simultaneous event, in Santos, Brazil, from 1 to 5 September, ...on Nuclear Physics was the fifth in a ...
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Lat. Am. j. solids struct. vol.10 número1
... Proceeding in the way analogous to that presented in Section 3.2, we may trace the limiting transition exhibited by relations of the equivalent heat transfer parameter defined by Eqs. (2.15), (2.16) for the ...
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