Phage display in vivo
Identificação de adesinas bacterianas por phage display
... de phage display é uma metodologia que vem sendo muito explorada e abriu caminhos para a construção de bibliotecas que apresentam ligantes de interesse na busca por alvos ...selecionar, in ...
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Phage display technology - Applications and innovations
... technique phage libraries are injected intrave- nously into animals and then organs or tissues are collected and examined for phage bound to tissue-specific endothe- lial cell markers (Figure ...endothelium ...
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IDENTIFICAÇÃO E CARACTERIZAÇÃO DE PEPTÍDEOS MIMÉTICOS DOS ANTÍGENOS DE Mycobacterium leprae POR PHAGE DISPLAY
... A tecnologia foi empregada com sucesso neste trabalho, uma vez que peptídeos foram caracterizados a partir da afinidade dos parátopos do anticorpo IgM às proteínas PIII da capa protéica dos fagomídeos, mimetizando a ...
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Clonagem e expressão de fragmentos funcionais(scFv) de anticorpo contra o Parvovírus canino-2 com a técnica da Phage display
... específicos ao antígeno inoculado, já que anticorpos específicos estão enriquecidos e passam por um processo de maturação in vivo pelo sistema imune do animal. (Benhar, 2001; Willats, 2002). Para isto, foi ...
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Screening of a specific peptide binding to VPAC1 receptor from a phage display peptide library.
... application in the molecular imaging of ...used in molecular imaging because it selectively binds to one subtype of VIPR with a higher affinity than ...performed in vivo pharmacokinetics and ...
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Tristetraprolin inhibits poly(A)-tail synthesis in nuclear mRNA that contains AU-rich elements by interacting with poly(A)-binding protein nuclear 1.
... a phage-display library for proteins that interact with tristetraprolin and retrieved, among others, a fragment of poly(A)- binding protein nuclear 1, which assists in the 3’-polyadenylation of mRNA ...
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Recombinant phages for Spodoptera frugiperda control: new perspectives to tackle an economic problem / Fagos recombinantes para o controle de Spodoptera frugiperda: novas perspectivas para enfrentar um problema econômico
... using Phage Display technology, seven recombinant phages were successfully selected and bound to intestinal ...SfF3 phage demonstrated similarity to the ABC transporter subfamily C2. In ...
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Caracterização de moléculas envolvidas no desenvolvimento de melanoma pela metodologia de Phage display
... de melanoma murino foi descrita pela primeira vez em nosso laboratório (Staquicini et al., submetido). Depleção de linfócitos por irradiação pode interferir no crescimento tumoral e estabelecimento de metástase ...
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Identification of human embryonic progenitor cell targeting peptides using phage display.
... Phage display is a powerful ligand selection method that has been applied both in vitro and in vivo for the identification of cell- specific targeting peptides ...on phage ...
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Antibody phage display libraries : contributions to oncology
... accumulation in a hVEGFR-3 expressing F9 teratocarcinoma in a mouse model by combined single proton emission computed tomography (SPECT/CT) and immunohistochemical ...targeted in other ways, such as ...
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A new peptide ligand for targeting human carbonic anhydrase IX, identified through the phage display technology.
... applied in order to improve labeling stability and reduce radioactive background ...[32]. In case of CaIX-P1 the high blood value is additionally explained by the metabolic properties of the ...experiments ...
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Selection of binding targets in parasites using phage-display and aptamer libraries in vivo and in vitro
... results in blood loss, dehy- dration, nutrient malabsorption, and increased susceptibility to other opportunistic ...which in turn release invasive ...challenge in veterinary science and, con- ...
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Sheep polyclonal antibody to map Haemonchus contortus mimotopes using phage display library
... evaluate phage display technology for mapping Haemonchus contortus ...PhD-7 Phage Display Peptide Library Kit with a sheep polyclonal antibody against ...50 phage peptide clones were ...
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SELEÇÃO DE PEPTÍDEOS INIBIDORES DA ENZIMA LesA DE Xylella
... Xylella fastidiosa (Xf) é uma bactéria Gram-negativa, restrita aos vasos do xilema dos hospedeiros e agente causal de doenças em diversas espécies de plantas cultivadas, sendo duas importantes: a Clorose Variegada dos ...
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Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library
... nal in osteosarcoma cells and compare it to the signal strength from normal controls in cell imaging approaches, such as immunofluorescence and ...antigen in the osteosarcoma cells was confirmed by ...
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Identificação e caracterização de peptídeos bioativos através de Phage display usando genoma completo de Corynebacterium pseudotuberculosis
... tool Phage Display was used that selects peptides using bacteriophages to express random sequences of peptides on their ...surface. Phage Display has been used successfully in many ...
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Epitope-based vaccines with the Anaplasma marginale MSP1a functional motif induce a balanced humoral and cellular immune response in mice.
... Prior to vaccination, we have demonstrated that peptides showed no toxicity to murine macrophage cells, ensuring that any observed effects were not due to peptides toxicity. The body weight was evaluated as a clinical ...
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SELEÇÃO DE PEPTÍDEOS RECONHECIDOS POR IMUNOGLOBULINAS Y ANTI-METACESTÓDEO DE Taenia solium POR PHAGE DISPLAY
... de phage-display esta técnica tem apresentado uma utilização cada vez mais crescente em diversas áreas das ciências, como a de definir peptídeos para testes ...
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Selection of specific colon cancer tumor peptides from combinatorial phage display libraries
... only in the selection of phage that has a growth advantage over the library ...wild-type phage will completely overtake the pool if too many rounds of amplification are carried out, regardless of the ...
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Genetic modification of a bacteriophage to create a multifunctional drug delivery particle
... grow, in the cover slips, for 24 ...temperature. In the end, cover slips were washed one last time with 1X PBS and transferred to 35 mm dishes (one dish per cover ...described in the section ...
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