Reverse transcriptase
Should we include connection domain mutations of HIV-1 reverse transcriptase in HIV resistance testing.
... HIV-1 reverse transcriptase (RT) confers a high level of resistance to lamivudine (3TC) and was also shown to counteract resistance to zidovudine (AZT) in the presence of thymidine analogue–associated ...
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PCR Amplification and Sequence Analyses of Reverse Transcriptase- like Genes in Crinipellis perniciosa Isolates
... Reverse transcriptase (RT) sequence analysis is an important technique used to detect the presence of transposable elements in a genome. Putative RT sequences were analyzed in the genome of the pathogenic ...
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A real-time reverse transcriptase polymerase chain reaction for detection and quantification of Vesiculovirus
... Vesiculoviruses (VSV) are zoonotic viruses that cause vesicular stomatitis disease in cattle, horses and pigs, as well as sporadic human cases of acute febrile illness. Therefore, diagnosis of VSV infections by reliable ...
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Evaluation of Viral Resistance to Reverse Transcriptase Inhibitors (RTI) in HIV-1- Infected Patients Before and After 6 Months of Single or Double Antiretroviral Therapy
... We evaluated samples of peripheral blood mononuclear (PBMC) cells from 46 AIDS patients, before starting therapy with HIV-1 reverse transcriptase inhibitors (RTI), and after 6 months of drug use. PBMC were ...
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Computational development of rubromycin-based lead compounds for HIV-1 reverse transcriptase inhibition
... HIV-1 reverse transcriptase using the efficient algorithm and simplified force field described by Hinsen ...HIV-1 reverse transcriptase (PDB: 2HMI) (Ding et ...empty reverse ...
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Mechanisms of human telomerase reverse transcriptase (hTERT) regulation: clinical impacts in cancer
... Telomerase was discovered in 1985, as an enzyme cap- able of extending telomeric repeat sequences; and in 1989, telomerase activity was reported for the first time [18–20]. However, the protein component of telomerase ...
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Micronuclei induced by reverse transcriptase inhibitors in mononucleated and binucleated cells as assessed by the cytokinesis-block micronucleus assay
... nucleoside reverse transcriptase inhibitors (zidovudine - AZT, lamivudine - 3TC and stavudine - d4T) using the cytokinesis-block micronucleus (CBMN) assay in human lymphocyte ...
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Diagnosis of Dengue by Using Reverse Transcriptase- Polymerase Chain Reaction
... The reverse transcriptase-polymerase chain re- action (RT-PCR) protocol developed by Lanciotti et al. (1992) could detect and identify all four serotypes of dengue viruses simultaneously in a nested ...
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Vitiligo: analysis of grafting versus curettage alone, using melanocyte morphology and reverse transcriptase polymerase chain reaction for tyrosinase mRNA
... through reverse transcriptase polymerase chain reaction, in tissue samples of vitiligo, before and after curettage, with or without subsequent autologous skin graft using a new ...
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INSILICO SCREENING OF NNRTI AS POTENTIAL TARGETS FOR TUMOR CONTROL
... Non-nucleoside reverse transcriptase inhibitors (NNRTIs) were identified as a new class of antiretroviral drugs over the past twenty years by means of dedicated research and development towards HIV ...
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Differentially displayed expressed sequence tags in Melipona scutellaris (Hymenoptera, Apidae, Meliponini) development
... Reverse transcription reactions were performed in triplicate using 200 ng of total RNA, 8 pmols of one of the oligo-DT primers containing an anchored base (Table I), 12U RNAsin (Amersham Biosciences), 1x RT ...
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Molecular detection of hepatitis E virus in feces and slurry from swine farms, Rio Grande do Sul, Southern Brazil
... nested reverse transcriptase-polymerase chain reaction (nested RT-PCR) in swine feces and effluents from slurry lagoons collected at the municipality of Teutônia, Rio Grande do Sul, the southernmost state ...
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DNA Damage and Its Cellular Response in Mother and Fetus Exposed to Hyperglycemic Environment
... Telomeric length and telomerase activity (a reverse transcriptase that limits telom- ere attrition) were studied in mononuclear cells isolated from umbilical cord blood of pregnant women[r] ...
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Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers.
... the reverse transcription by SuperScriptH III (Figure S1, supporting ...M-MuLV Reverse Transcriptase was also able to produce the full-length cDNA, however, it was not as efficient as SuperScriptH ...
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Sensitive Tumorigenic Potential Evaluation of Adult Human Multipotent Neural Cells Immortalized by hTERT Gene Transduction.
... Stem cells and therapeutic genes are emerging as a new therapeutic approach to treat vari- ous neurodegenerative diseases with few effective treatment options. However, potential formation of tumors by stem cells has ...
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Clinical and Biochemical Evaluation of HIV-Related Lipodystrophy in an Ambulatory Population from the Hospital Universitário Cassiano Antonio de Morais, Vitória, ES, Brazil
... We designed a retrospective cohort study to identify factors associated with HIV-1 related lipodystrophy at a tertiary HIV-care center in Vitória, ES, Brazil. Inclusion criteria were documented HIV diagnosis, ...
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Prevalência de resistência transmitida do HIV-1 aos antirretrovirais no Brasil, pré- início de tratamento
... of reverse transcriptase and protease, with mutational analysis of the profile by a population patients chronically infected with HIV-1 and not treated, but with indication of immediate initiation of ...
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The hepatitis B virus ribonuclease H is sensitive to inhibitors of the human immunodeficiency virus ribonuclease H and integrase enzymes.
... Nucleos(t)ide analog therapy blocks DNA synthesis by the hepatitis B virus (HBV) reverse transcriptase and can control the infection, but treatment is life-long and has high costs and unpredictable ...
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HIV-1 SUBTYPES AND MUTATIONS ASSOCIATED TO ANTIRETROVIRAL DRUG RESISTANCE IN HUMAN ISOLATES FROM CENTRAL BRAZIL Daniela Marreco Cerqueira1 , Eduardo Dias Ramalho1 ; Claudiner Pereira Oliveira1 ; Ruiter Roberto Silva2; Miriam Franchini2 ; Maria Sueli Soare
... The detection of polymorphisms associated to HIV-1 drug-resistance and genetic subtypes is important for the control and treatment of HIV-1 disease. Drug pressure selects resistant variants that carry mutations in the ...
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