ribosomal RNA
Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species
... evaluate ribosomal RNA gene sequenc- ing for the identification of medically relevant Candida species in comparison with a standard phenotypic ...26S ribosomal RNA gene regions were used as ...
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Basonuclin regulates a subset of ribosomal RNA genes in HaCaT cells.
... cell-type-specific ribosomal RNA (rRNA) gene regulator, is expressed mainly in keratinocytes of stratified epithelium and gametogenic cells of testis and ...of RNA polymerase I (Pol I) transcription ...
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Epidemiology, biochemistry and evolution of Trypanosoma cruzi lineages based on ribosomal RNA sequences
... In order to analyse the activity of Lineage 2 rRNA promoter, we cloned the corresponding seg- ment from the Dm28 strain. Alignment of this se- quence (800bp) with rRNA promoters from other strains of T. cruzi indicated ...
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Characterization of primary biogenic aerosol particles in urban, rural, and high-alpine air by DNA sequence and restriction fragment analysis of ribosomal RNA genes
... the ribosomal RNA genes. These are the 16S ribosomal genes in the domains Archaea and Bacteria, and the 18S ribosomal genes in the domain Eu- karya (animals and ...
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Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold.
... 50S ribosomal RNA-binding protein L35Ae from the extremophilic archaea Pyrococcus horikoshii has been probed for its potential to serve as a backbone in alternative scaffold ...
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Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing
... Based on the sequences of strains QAU-63 and QAU-68, BLAST search results showed that the both strains are more closely related to the species of genus Bacillus (Figure 2) with 95.75% an[r] ...
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WDR55 is a nucleolar modulator of ribosomal RNA synthesis, cell cycle progression, and teleost organ development.
... At 2 days after WDR55-siRNA transfection into NIH3T3 cells, the expression of p21 Waf1/Cip1 , a gene that is regulated by p53 and controls cell cycle [36,37], was significantly increased, while that of WDR55 mRNA was ...
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Mapping of the 18S and 5S ribosomal RNA genes in Astyanax altiparanae Garutti Britski, 2000 (Teleostei, Characidae) from the upper Paraná river basin, Brazil
... inactive ribosomal DNA (rDNA) and almost always allows detection of a larger number of NORs than can be detected using Ag-NOR banding and is also more precise in identifying ...
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Evaluation and selection of candidate reference genes for normalization of quantitative RT-PCR in Withania somnifera (L.) Dunal.
... 18S ribosomal RNA (18S-rRNA), 26S ribosomal RNA (26S), Actin (ACT), cyclophylin (CYP), elongation factor-1 (EF-1), glyceral-dehyde-3-phosphate de- hydrogenase (GAPDH), ribosomal protein ...
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A non-hepatotropic parasite infection increases mortality in the acetaminophen-induced acute liver failure murine model: possible roles for IL-5 and IL-6
... Quantification of IL-5 and IL-6 expression by re- verse transcriptase polymerase chain reaction (RT- PCR) - To quantify the mRNA expression of IL-5 and IL-6, total RNA samples from hepatic tissues were re- ...
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Trichoderma spp. in Garlic and Onion Fields and In Vitro Antagonism Trials on Sclerotium cepivorum
... The hypotheses was that Trichoderma is a genus present in the onion and garlic’s rhizosphere in soil in Costa Rica, and the isolates obtained had different levels of antagonistic activity against S. cepivorum . The ...
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First report of Xiphinema brevicolle Lordello et Costa, 1961 (Nematoda, Longidoridae) in Japan
... his study was conducted using morpho- logical characters of females and DNA sequences of the mitochondrial COI region and the nuclear ribosomal RNA (rDNA) regions to identify and charac[r] ...
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MATERIALS AND METHODS Bacterial Strains
... The multiplex PCR technique described in this study has proven to be effective for the R. equi molecular characterization. Additionally, the epidemiological status (endemic, sporadic or no infection) of horse breeding ...
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Genetic diversity, morphological uniformity and polyketide production in dinoflagellates (Amphidinium, Dinoflagellata).
... the ribosomal RNA (rRNA) operon, has shown that high levels of genetic diversity exist within morphologically indistinguishable species of dinoflagellates ...
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A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography
... for RNA isolation from a biological source, because of its unique char- acteristic of using a biospecific ligand to purify biomolecules, on the basis of their biological function or individual chemi- cal ...of ...
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Significance of anaerobes and oral bacteria in community-acquired pneumonia.
... Methods: Patients with community-acquired pneumonia were enrolled based on their clinical and radiological findings. Bronchoalveolar lavage specimens were collected from pulmonary pathological lesions using bronchoscopy ...
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Ticks and Tick-borne Diseases
... Quantitative PCR studies were designed according to the MIQE guidelines (Bustin et al., 2009). Quantitative polymerase chain reac- tions (qPCR) were run on all genes to determine optimal reaction conditions, PCR ...
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Variability in Ribosomal DNA Genic and Spacer Regions in Verticillium dahliae Isolates from Different Hosts
... the ribosomal DNA intergenic spacer region (IGS) and a portion of the mitochondrial DNA small subunit ribosomal RNA gene (mtDNA SSU rRNA), the genetic variability among Verticillium dahliae isolates ...
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Multiplex TaqMan qPCR assay for specific identification of encapsulated Trichinella species prevalent in North America
... Oligonucleotide design and TaqMan real-time PCR optimization - Generic ribosomal RNA (rRNA) gene sequences of the different nematode species, including Trichinella spp. available in GenBank were aligned and ...
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Molecular Characterisation of Intermediate Snail Hosts and the Search for Resistance Genes
... The relationship between schistosomes and their intermediate hosts is an extremely intricate one with strains and species of the parasite depending on particular species of snail, which in turn may vary in their ...
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