Rpob Gene
Low-stringency single-specific-primer PCR as a tool for detection of mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis
... 9. Taniguchi, H., H. Aramaki, Y. Nikaido, Y. Mizuguchi, M. Nakamura, T. Koga, and S. Yoshida. 1996. Rifampicin resistance and mutation of the rpoB gene in Mycobacterium tuberculosis. FEMS Microbiol. Lett. ...
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Multilocus Sequence Analysis and rpoB Sequencing of Mycobacterium abscessus (Sensu Lato) Strains
... of rpoB gene sequencing for the molecular identification of iso- lates and discrimination between the different groups of ...on rpoB sequencing (31, 34, 47, ...the rpoB gene between the ...
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Characterization of Mycobacteria from a Major Brazilian Outbreak Suggests that Revision of the Taxonomic Status of Members of the Mycobacterium chelonae-M-abscessus Group Is Needed
... Genetic sequencing of the rpoB region, as proposed by Adekambi et al. (2) for the separation of rapidly growing mycobacteria, confirmed the high degree of heterogeneity within clinical isolates of M. abscessus, ...
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Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates
... hsp65 gene (PRA-hsp65), called Mycobacterium absces- sus type 2 by Devallois et ...the rpoB gene (2, 19, 28); and two highly similar patterns with one band difference when they were typed by ...
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Characterization of Francisella species isolated from the cooling water of an air conditioning system
... rRNA gene, and phylogenetic and BLAST analyses with multiple genes were consistent in differentiating the 9 strains of ...the rpoB gene, strains of cluster 1 were also 100% iden- tical to ...
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Correlation between the BACTEC MGIT 960 culture system with Genotype MTBDRplus and TB-SPRINT in multidrug resistant
... clinical specimens, such as sputa, avoiding delay due to specimen treatment and DNA extraction and providing the so called “sample-to-answer” solution. However, the MTBDRplus and Xpert MTB/RIF require expensive equipment ...
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Pre-existing isoniazid resistance, but not the genotype of Mycobacterium tuberculosis drives rifampicin resistance codon preference in vitro.
... Both the probability of a mutation occurring and the ability of the mutant to persist will influence the distribution of mutants that arise in a population. We studied the interaction of these factors for the in vitro ...
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Diversidade bacteriana do solo em diferentes fitofionomias do bioma cerrado e perspectivas biotecnológica
... The versatility of the microbial community is very important for biotechnology because the soil microorganisms have been the source of new products, including antibiotics and enzyme production. A Paenibacillus genus ...
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Genetic and proteomic characterization of rpoB mutations and their effect on nematicidal activity in Photorhabdus luminescens LN2.
... resulting rpoB fragments were purified and ligated into the suicide vector pPHU281 [50] digested with BamHI and PstI to yield plasmids pPHU281-rpoB- LW and ...mutant rpoB allele from LN2-R31) and ...
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Molecular characterization of Mycobacterium massiliense and Mycobacterium bolletii in isolates collected from outbreaks of infections after laparoscopic surgeries and cosmetic procedures
... hsp65 gene with BstEII of bands of 235 and 210 bp and with HaeIII of bands of 200, 70, 60, and 50 bp, which is common to Mycobacterium abscessus type 2, Mycobac- terium bolletii, and Mycobacterium ...and ...
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Mutations in rpoB and katG genes in Mycobacterium isolates from the Southeast of Mexico
... The most frequent mutations associated with rifampin and isoniazid resistance in Mycobacterium are the sub- stitutions at codons 531 and 315 in the rpoB and katG genes, respectively. Hence, the aim of this study ...
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Plasmidbased controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reactionhighresolution melting
... the rpoB gene with qPCR-HRM using plasmid-based ...the rpoB were cloned into pGEM-T vector and these vectors were used as controls in the qPCR-HRM analysis of 54 ...
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Pleural effusion of patients with tuberculosis is characterized by accumulation of gamma delta T lymphocytes that expresses distinct surface markers
... in rpoB gene were revealed in ...katG gene, was revealed in ...inhA gene in 43(23.1%) patients. The ahpC gene has low- est number of mutations - ...studied gene. In 3 gene ...
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Identification of Leptospira serovars by RFLP of the RNA polymerase beta subunit gene (rpoB)
... the rpoB gene, which encodes the beta-subunit of RNA polymerase, was used as an alternative tool to identify ...the rpoB gene was amplified by polymerase chain reaction ...the rpoB ...
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Clonal multidrug-resistant Corynebacterium striatum within a nosocomial environment, Rio de Janeiro, Brazil
... and rpoB gene ...rRNA gene was amplified using universal primers pA (5’-AGAGTTTGATCCTGGCTCAG) and pH (5’-AAG- GAGGTGATCCAGCCGCA), as described by Watts et ...The rpoB gene was amplified ...
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Detection of rifampin-resistant genotypes in Mycobacterium tuberculosis by reverse hybridization assay
... Comparision of CRDH with DNA sequencing - Out of 53 M. tuberculosis strains that had mutations in the hot-spot region of the rpoB gene according to sequenc- ing, 48 (90.6%) were correctly detected by the ...
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The rationale for using rifabutin in the treatment of MDR and XDR tuberculosis outbreaks.
... The 34 clinical isolates were phenotypically susceptible to RFB as per CC, despite of their resistance to RIF and the presence of SNPs at codon 516 in the rpoB gene (Table 1). However, a shift in the RFB ...
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A gene wiki for community annotation of gene function.
... Wikipedia gene network has at least two ...Wikipedia gene network facilitates the efficient “browsing” of related topics in the local gene ...in gene wiki connectivity has potential ...
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POSTULAÇÃO DE GENES (Lr) DE RESISTÊNCIA À FERRUGEM DA FOLHA EM CULTIVARES BRASILEIRAS DE TRIGO
... cada gene que condiciona uma reação de resistência no hospedeiro existe um gene complementar, no patógeno, que condiciona ...conceito gene-a-gene e na genética das associações ...
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Einstein (São Paulo) vol.13 número1
... polymorphism characterizes these genes (2,735 alleles at HLA-A, 3,455 alleles at HLA-B and 2,259 alleles at HLA-C loci), and the vast majority of these alleles are functional. There are also so-called non-classic HLA ...
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