Site-Directed
Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile
... A site-directed mutant R453T of a laccase from hermus thermophilus HB27 (Tth-laccase) was constructed in order to investigate the efect on laccase catalytic ...
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A structure-based site-directed mutagenesis study on the neurolysin (EC 3.4.24.16) and thimet oligopeptidase (EC 3.4.24.15) catalysis
... The QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA) was used to introduce speci¢c point mutations in the wild type EP24.15 or EP24.16 cDNA, as previously described [11]. Point ...
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Membrane docking geometry of GRP1 PH domain bound to a target lipid bilayer: an EPR site-directed spin-labeling and relaxation study.
... The previously described optimized, fully functional Cysless GRP1 human PH domain (residues 255–392 and C293S/C327A/ C343S) was employed as the background for creation of a single- Cys mutant library [26,27]. Single ...
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Identification of residues involved in nucleotidyltransferase activity of JHP933 from helicobacter pyloriby site-directed mutagenesis
... rational site- directed mutagenesis and enzymatic assay, we further pinpointed the residues key to JHP933’s nucleotidyl transferring function and confirmed the active site for JHP933, thus providing ...
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Characterization and regulation of a bacterial sugar phosphatase of the haloalkanoate dehalogenase superfamily, AraL, from Bacillus subtilis
... using site-directed mutagenesis, we show that the production of AraL is regulated by a structure in the translation initiation region of the mRNA, which most probably blocks access to the ribosome-binding ...
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Heterologous Proteins Production in Escherichia coli: An Investigation on the Effect of Codon Usage and Expression Host Optimization
... by site-directed mutagenesis and the expression level of the recombinant protein is analyzed by means of sodium dodecyl sulfate polyacry- lamide gel electrophoresis (SDS-PAGE) and Western ...
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Alpha-synuclein phosphorylation role on Parkinson’s disease
... The SynT aggregation model was used to study the effect of Y125F and S129G mutations on aSyn inclusion formation.. The SynT plasmids were constructed by site-directed muta[r] ...
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Structural and Mechanistic Studies of Prokaryotic Ferritins
... In order to understand the functionality and DNA binding properties of Dps through the N-terminal region, site-directed spin labelling (SDSL) [190,192] combined with pulsed electron-electron double ...
226
Defining the functional domain of programmed cell death 10 through its interactions with phosphatidylinositol-3,4,5-trisphosphate.
... using site-directed mutagenesis (QuikChange, Stragetagene) including two K-to-A mutations (D2KA:K169A, and K172A), three K-to-A mutations (D3KA:K179A, K183A and K186A), and five K-to-A mutants (D5KA: K169A, ...
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A sucrose binding protein homologue from soybean exhibits GTP-binding activity that functions independently of sucrose transport activity
... by site-directed mutagenesis the GTP binding site of S64/SBP2 to this imperfect A consensus ...binding site (Thr283Leu and Lys284Glu changes) do not represent structurally neutral ...the ...
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ISSN 0100-879X BIOMEDICAL SCIENCES AND CLINICAL INVESTIGATION
... A pT7-7 vector coding the wild-type Sfβgly was used as a template in site-directed mutagenesis experiments employing the kit “QuikChange site directed mutagenesis” (Stratagene, USA). The ...
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Initiation of RNA Polymerization and Polymerase Encapsidation by a Small dsRNA Virus.
... active site (Fig 1A). Through site-directed mutagenesis, we determined that the loop structure most likely functions as a priming platform to support the binding of a single priming nucleotide (Fig ...
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Revealing a Novel Otubain-Like Enzyme from Leishmania infantum with Deubiquitinating Activity toward K48-Linked Substrate
... and site-directed mutations on amino acids close to the catalytic site (F82) or involved in Ub interaction (L265 and F182) caused structural changes as shown by molecular dynamics, resulting in a ...
11
Dissection of the TssB-TssC interface during type VI secretion sheath complex formation.
... Leu, Ile) while the opposite face is composed of polar or positively charged residues (Arg, Gln). The charge partition in the C- terminal region of the helix is better balanced but we observed a leucine-rich face, on the ...
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Purification, cloning, characterization and essential amino acid residues analysis of a new ι-carrageenase from Cellulophaga sp. QY3.
... subsites. Site-directed mutagenesis followed by kinetic analysis identified three strictly conserved residues at subsites 21 and +1 of i-carrageenases, G228, Y229 and R254 in CgiA_Ce, which played important ...
11
Development of constructs for recombinant expression of human follicle-stimulating hormone receptor in rod cells of the zebrafish
... reactions, site-directed mutagenesis and other molecular biological methods, we were able to construct mutated receptors with different signal sequences and different tags, including the last fifteen amino ...
80
Functional Domains of the Bacillus subtilis Transcription Factor AraR and Identification of Amino Acids Important for Nucleoprotein Complex Assembly and Effector Binding†
... Negative transdominance of AraR mutants. B. subtilis strain IQB101 (wild-type araR), harboring an araAB⬘-lacZ fusion, was transformed with the plasmids encoding selected AraR mutants obtained by ...
13
Structural insights into cellulolytic and chitinolytic enzymes revealing crucial residues of insect β-N-acetyl-D-hexosaminidase.
... overall topology as well as amino acid sequence shares very little similarity (Fig. 1) [8]. Insect OfHex1 from the Asian corn borer (Ostrinia furnacalis) is a b-N-acetyl-D-hexosaminidase specialized for chitin ...
9
Novel enzyme-polymer conjugates for biotechnological applications
... the site-directed incorporation of tailor-made polymers into protein to create biocatalysts with excellent properties for pharmaceutical industrial purpose has been ...the site-directed ...
17
Intergenic incompatibilities reduce fitness in hybrids of extremely closely related bacteriophages
... known site of contact between the coat protein and the external scaffolding protein (D) during the procapsid stage of assembly (Dokland et ...by site-directed mutagenesis by substituting this ...
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