Specific primers
Highly parallel and short-acting amplification with locus-specific primers to detect single nucleotide polymorphisms by the DigiTag2 assay.
... locus specific primers sufficiently worked in a multiplex form under the same reaction conditions without any optimization processes, either 96-plex PCR or 192-plex ...locus-specific primers ...
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Rapid plant identification using species- and group-specific primers targeting chloroplast DNA.
... Plant identification is challenging when no morphologically assignable parts are available. There is a lack of broadly applicable methods for identifying plants in this situation, for example when roots grow in mixture ...
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PCR WITH Paracoccidioides brasiliensis SPECIFIC PRIMERS: POTENTIAL USE IN ECOLOGICAL STUDIES
... The precise microenvironment of Paracoccidioides brasiliensis has not yet been discovered perhaps because the methods used are not sensitive enough. We applied to this purpose the polymerase chain reaction (PCR) using ...
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Specific genotyping of human leukocyte antigen-A*01 by polymerase chain reaction using allele group-specific primers
... pletely complementary to HLA-A*0103, HLA-A*0106 or HLA-A0107. Moreover, other HLA-A group alleles (e.g., HLA-A*3603, HLA-A*8001) are matched to this sequence and amplified by these primers. In this study, however, ...
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Detection of Metarhizium anisopliae var. anisopliae within infected sugarcane borer Diatraea saccharalis (Lepidoptera, Pyralidae) using specific primers
... oligonucleotide primers in the design of these systems is not useful in the detection of fungi within environmental samples which can contain a significant amount of DNA from indigenous biotic materi- ...
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Evaluating primers for profiling anaerobic ammonia oxidizing bacteria within freshwater environments.
... PCR primers for the detection of anammox bacteria within freshwater ecosystems, we analyzed representative positive controls and selected samples from Grand River and groundwater sites, both from Ontario, ...
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DISSERTACAO Desenvolvimento de primers para detecção de Xanthomonas axonopodis pv. malvacearum
... of specific primers for detection of Xam in cotton ...such, primers were designed and tested for specificity and sensitivity in nineteen strains of Xam belonging to the States MG, SP, GO, MT e PR, ...
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Comparative study on the use of specific and heterologous microsatellite primers in the stingless bees Melipona rufiventris and M. mondury (Hymenoptera, Apidae)
... these primers have revealed a low level of polymorphism, possibly the consequence of the heterologous primers used, since in most cases these were not specifically designed for the species under ...
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Assessment of primers designed from the small ribosomal subunit RNA for specific discrimination between Babesia bigemina and Babesia bovis by PCR
... species-specific primers were designed from the alignment of the sequences of the SS rRNA gene obtained from the Genbank database for Babesia bigemina (accession number X59604) and for ...of primers ...
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Análise de diferentes primers utilizados na PCR visando ao diagnóstico da tuberculose no Estado do Amazonas.
... The primers specific for amplifying the 123 bp IS6110 fragment gave the highest positivity ...the specific primers used to amplify the 123bp IS6110 fragment showed a greater efficiency in ...
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Family-specific vs. universal PCR primers for the study of mitochondrial DNA in plants
... of primers which are specific for groups of more closely related species, such as families, assumed to have similar evolution and organization of their cytoplasmic genomes ...family- specific ...
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Descrição de marcadores InDel ligados ao cromossomo X com uso possível de primers internos
... a specific sequence in PCR, by designing primers that anneal to the regions of interest and amplify specifics alleles in that locus, which improves the DNA detection and quantification in DNA mixtures ...
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Transferibilidade de primers heterólogos em Brycon falcatus
... species-specific primers. However, pairs of primers designed for one species can be used for cross-amplification in phylogenetically related species, allowing genetic studies to use transferred ...
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Obtenção de primers microssatélite e desenvolvimento, validação e mapeamento de marcadores SCAR em feijoeiro-comum
... que primers específicos sejam desenhados para estas regiões (YU et ...Estes primers podem ser utilizados, para amplificar fragmentos a partir do DNA de diferentes ...
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NEW PRIMERS FOR DETECTION OF Leishmania infantum USING POLYMERASE CHAIN REACTION
... or specific. Our aim was to design new primers to perform a Polymerase Chain Reaction (PCR) for detecting ...FLC2/RLC2 primers were ...FLC2/RLC2 primers amplified a fragment of 230 bp and the ...
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DESENVOLVIMENTO DE PRIMERS IN SILICO DE Mycobacterium tuberculosis DA REGIÃO 16S PARA O DIAGNÓSTICO DA TUBERCULOSE
... Durante esse processo, utiliza-se majoritariamente a ferramenta OligoAnalyzer para analisar os aspectos termodinâmicos da sequência de primer. Esta técnica evidencia o padrão exergônico da reação, por meio do valor do ∆G ...
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RAPD profile among Candida albicans isolates by using different primers
... In our study we observed that the discriminatory power of RAPD assays rest upon the nature of the primer. According to Dassanayake et al. (4) the resolution of PCR mediated typing of Candida strains depends heavily on ...
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Type-specific cell line models for type-specific ovarian cancer research.
... Regardless of origin or histological similarities and differences, biomarker and genomic studies have been successfully used to distinguish each histotype and may represent a far more biologically relevant basis for ...
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Design and implementation of degenerate microsatellite primers for the mammalian clade.
... comparative primers useful across the Mammalia, then many more are expected to be developed from more specific comparisons, ...degenerate primers (low amplifica- tion), and direct PCR sequencing (low ...
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Construção de pares de primers para a detecção espécie-específica de Leishmania (Leishmania) infantum chagasi por PCR
... using primers targeting kDNA of the Leishmania can be explained by the large number of copies of DNA present in the kinetoplast minicircle (REALE et ...using primers for kDNA targets, Smyth et ...that ...
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