substrate specificity
Profiling of substrate specificity of SARS-CoV 3CL.
... of substrate specificity of the protease, a substrate library of 19|8 variants was created by performing saturation mutagenesis on the autocleavage sequence at P5 to P3’ ...The substrate ...
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Identifying protein phosphorylation sites with kinase substrate specificity on human viruses.
... kinase substrate specificities, human phosphorylated proteins annotated with their catalytic kinases are collected from ...visualized substrate specificity of human kinases are compared. A ...
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Identification of crucial amino acids in mouse aldehyde oxidase 3 that determine substrate specificity.
... Additionally, XOR and AOX enzymes act on different, yet overlapping sets of substrates, and XOR enzymes usually show higher rate constants towards their specific substrates. Here, we present for the first time the ...
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Molecular mechanism of strict substrate specificity of an extradiol dioxygenase, DesB, derived from Sphingobium sp. SYK-6.
... broad substrate specificity, DesB has strict substrate specificity for ...The substrate specificity of DesB seems to be required for the efficient growth of ...the ...
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ZapA, a possible virulence factor from Proteus mirabilis exhibits broad protease substrate specificity
... the substrate specificity of this enzyme has not yet been fully ...broad specificity, demonstrated by the presence of hydrophobic, hydrophilic, charged and uncharged amino acid residues at the ...
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Regulation of AKT phosphorylation at Ser473 and Thr308 by endoplasmic reticulum stress modulates substrate specificity in a severity dependent manner.
... AKT substrate specificity in a severity-dependent manner, as shown by phospho-specific antibodies against known AKT ...Thr308. Substrate specificity is again ...
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Serine 363 of a Hydrophobic Region of Archaeal Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase from Archaeoglobus fulgidus and Thermococcus kodakaraensis Affects CO2/O2 Substrate Specificity and Oxygen Sensitivity.
... To assess the importance of Ser-363, in the current study mutations were made in archaeal RubisCO at this residue. Ser-363 resides in a hydrophobic pocket in which many of the constitu- ent hydrophobic residues are ...
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Substrate specificity and the effect of calcium on Trypanosomabrucei metacaspase 2
... extended substrate specificity and effects of Ca 2+ on the activation of these enzymes, detailed kinetic, biochemical and structural analyses were carried out on metacas- pase 2 from Trypanosoma brucei ...
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Substrate specificity within a family of outer membrane carboxylate channels.
... are substrate specific for low-molecular weight compounds (,200 Da), we anticipated that the specificity might be even more pronounced for the higher molecular weight antibiotics (,300–500 Da; Figure ...
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A detailed analysis of the murine TAP transporter substrate specificity.
... the specificity matrix calculated by us for murine TAP is in good qualitative agreement with previously published experimental data generated with model ...
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Crystal structure and substrate specificity of D-galactose-6-phosphate isomerase complexed with substrates.
... different substrate orientation than that in RpiB, where the substrate binds at ...and substrate orientation, and Cys-65 is essential for the isomerization activity of the ...
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Substrate specificity of kallikrein-related peptidase 13 activated by salts or glycosaminoglycans and a search for natural substrate candidates
... KLK13 is a kallikrein-related peptidase preferentially expressed in tonsils, esophagus, testis, salivary glands and cervix. We report the activation of KLK13 by kosmotropic salts and glycosaminoglycans and its ...
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Yellow fever virus NS2B/NS3 protease: Hydrolytic Properties and Substrate Specificity
... the substrate was hydro- lyzed over the course of the ...of substrate hydrolyzed per minute based on a calibration curve obtained from the complete hydrolysis of each ...
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Evolution of acetylcholinesterase and butyrylcholinesterase in the vertebrates: an atypical butyrylcholinesterase from the Medaka Oryzias latipes.
... ChE specificity is the acyl pocket, which in vertebrate AChE is characterized by two Phe residues (Phe288 and Phe290 in Torpedo ...intermediate substrate specificity of the ...
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Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability.
... A new Cerrena sp. strain HYB07 was reported, which produced over 200 U mL 21 laccase activity after 3-d cultivation in the shaking flask. The high laccase yield and short production period of HYB07 are advantageous for ...
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Crystal structures of the novel cytosolic 5'-nucleotidase IIIB explain its preference for m7GMP.
... The overall structure of Dm cN-IIIB consists of two domains: the globular core domain and a smaller cap domain. The globular core domain is a haloacid dehalogenase domain (HAD domain) and resembles a Rossmannoid-like a/b ...
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Cleavage entropy as quantitative measure of protease specificity.
... Protease Specificity We therefore surmise that a detailed understanding of protease specificity is only in reach within an even smaller subset of homologue proteases, where changes in substrate ...
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PRODUCTION OF THERMOSTABLE GLUCOAMYLASE BY NEWLY ISOLATED ASPERGILLUS FLAVUS A 1.1 AND THERMOMYCES LANUGINOSUS A 13.37
... better substrate than corn starch for glucoamylase production by ...the substrate used was a mixture of starch and maltose, suggesting that this fungus produced two types of ...the substrate ...
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The use of Fluorescence Resonance Energy Transfer (FRET) peptidesfor measurement of clinically important proteolytic enzymes
... Proteolytic enzymes have a fundamental role in many biological processes and are associated with multiple patholog- ical conditions. Therefore, targeting these enzymes may be important for a better understanding of their ...
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Molecular cloning and characterization of taurocyamine kinase from Clonorchis sinensis: a candidate chemotherapeutic target.
... characterize substrate recognition property of ...with substrate binding, as revealed by the CK and AK crystal ...guanidine substrate-binding site [33], [34], [35], and functionally, this residue ...
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