[PDF] Top 20 Differentiation of human pluripotent stem cells to the neuronal dopaminergic fate

... É uma doença rara e hereditária que se deve à deficiência da enzima glucocerebrosidade. Esta deficiência leva ao armazenamento excessivo de glucosilceramida no fígado, baço, osso e medula óssea. Além disso, pacientes com ... See full document

... ability to direct ES and induced pluripotent stem (iPS) cell differentiation toward specific tissue fates in vitro provides an excellent opportunity to investigate the gene ... See full document

... commitment of a specialized cell type from induced pluripotent stem cells (iPSCs) without contamination from unknown substances is crucial to their use in clinical ...progenitor ... See full document

... IPS to a range of sources, including epigenetic memory [5–10,17], inherent differences between IPSCs and ESCs [4,28], artifacts of reprogramming [7] or lab environment ...surprisingly, the ... See full document

... penicillin-streptomycin). The NSSs were sub-cultured every week using a McIlwain tissue chopper (Mickle Engineering, Gomshall, Surrey, UK), and the medium was refreshed every 2 ...terminal ... See full document

... induction of hESCs based on co-culture with MS5 or PA6 stromal cells have been reported [8, 16, 20, ...HSF6 cells differentiated into neural rosette-like cells, which are primitive neuroe- ... See full document

... Ki67-ir cells, mean6SEM, n = 10, two indepen- dent ...cultures, differentiation at low oxygen tension resulted in a significantly higher percentage of proliferating cells as compared to ... See full document

... induced pluripotent stem cells (hiPSCs) generated by de-differentiation of adult somatic cells offer potential solutions for the ethical issues surrounding human ... See full document

... led to efficient motoneuron generation from iPS and E14 cell ...found to be Hb9-positive (Fig. 5A3). C57-iPS-12 cells showed similar potency in motoneruon ...in the percentage of both ... See full document

... iPS Cells: Human induced pluripotent stem cells (iPSCs, cat# SC102A-1) were generated by transducing genetically unmodified human fibroblasts with viruses individually encoding ... See full document

... hiPS cells were differentiated on the R-Fc-coated surface, we detected the expression of several markers of hepatic lineage cells (Fig 6B), and differentiated cells on ... See full document

... [2]). The retroviral construct pRV-IRES-EGFP (referred to simply as the EGFP vector) was used as a control in infection ...experiments. The human Gsx2 ORF (1 Kb) was amplified from a ... See full document

... during differentiation to oligodendrocytes. The percentage of antigen-expressing cells measured by immuno- fluorescence staining exhibited no significant difference between ... See full document

... profiling of cell lysates and conditioned culture medium showed an increase in vascular endothelial growth factor for CO-treated cultures (cell lysates) compared to controls (Fig ...investigated the ... See full document

... According to our results, erythroid cells ex- pressed high levels of fetal ...remarkably, the expression level of γ-globin was ...related to the presence of several ... See full document

... use of experimental animals in this study was conducted in accordance with the recom- mendations in the Guiding Principles for the Care and Use of The Japanese Pharmacological ... See full document

... positive cells could be detected in long-term culture of hPSCs on Matrigel matrix together with mTeSR1 medium than in MEF and SNL feeder cell ...cultures. The expression of PAX-6, Musashi and ... See full document

... rejection of syngeneic mouse iPSCs (miPSCs) following transplantation [17], in which miPSCs derived via the episomal approach were less prone to immune-mediated attack than those generated using ... See full document

... colonies of undifferentiated hES cells, 800–1000 mm in diameter, were picked up from the feeder layer using a glass capillary and transferred into non-adhesive bacteriological dishes each containing ... See full document

... that the isolated MSCs are positive for CD44, CD73, CD90, and CD105 and negative for the hemato- poietic markers CD3, CD14, CD19, CD34, and ...and neuronal structures ...blood-derived cells in ... See full document