[PDF] Top 20 Expression of the CTCF gene in bovine oocytes and preimplantation embryos
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Expression of the CTCF gene in bovine oocytes and preimplantation embryos
... pools of 50 embryos and from 50 oocytes using the RNeasy Mini Kit (Qiagen, USA) and from peripheral blood with Trizol (Invitrogen, Brazil), fol- lowing the manufacturer’s ... See full document
4
Annexin II mRNA expression in bovine oocytes during follicular development
... µM of each dNTP, 0.4 µM of each primer, 2.5 U Taq DNA polymerase and 3µL of cDNA in a final volume of 25 ...performed in a thermocycler under the following ... See full document
5
Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue
... that the presence of MATER and ZAR1 in oocytes is needed for further embryo development, since null mutant mice embryos arrest cleavage at the 1- or 2-cell stage (Tong et ... See full document
8
Oocyte activation and preimplantation development of bovine embryos obtained by specific inhibition of cyclin-dependent kinases
... use of broad-spectrum inhibitors of protein synthesis or phosphorylation, such as cycloheximide or 6-DMAP, respectively, have allowed high rates of parthenogenetic activation of bovine ... See full document
8
Gene expression studies on bovine oocyytes and embryos subjected to various heat stress and heat shock conditions
... affects the dairy cattle to experience either heat stress or cold ...to the seasonal effect on reproduction in dairy ...to the low fertility of dairy cows during summer months ( Ray et ... See full document
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Characterization of the IGF2 Imprinted Gene Methylation Status in Bovine Oocytes during Folliculogenesis.
... involved in chromatin remodeling at imprinted and non-imprinted regions of the ...acquired in a sex- specific manner during oogenesis and spermatogenesis ...for the ... See full document
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Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes.
... Regarding the length of CNT, some studies have shown that MWNT with longer length have greater tox- icity than smaller ones 43 ...that the shorter MWNT caused more toxicity than longer-length MWNT 44 ... See full document
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Chromatin modifying agents in the in vitro production of bovine embryos
... reports of utilizing HDAC inhibitors were in cancer ...Inhibitors of HDAC promote global chromatin acetylation, which leads to excessive gene ...increase in protein production in ... See full document
10
Oocytes, embryos and pluripotent stem cells from a biomedical perspective
... refined and characterized a non- integrative episomal plasmid-based human iPSC reprogramming strategy first published by Okita et ...on the use of electroporation of fibroblasts with three ... See full document
16
Dynamics of DNA Methylation during Early Development of the Preimplantation Bovine Embryo
... divergence in control of DNA methylation during preimplantation ...development. The exact pattern of methylation in the bovine embryo has not been established nor ... See full document
10
Reference gene selection for gene expression analysis of oocytes collected from dairy cattle and buffaloes during winter and summer.
... (10–20 oocytes) to obtain reliable results ...analyze gene expression in oocytes or embryos [37,41,45–49], mostly because of the general belief that this method ... See full document
13
Expression profile of genes as indicators of developmental competence and quality of in vitro fertilization and somatic cell nuclear transfer bovine embryos.
... using the PolyA Tract mRNA Isolation System (Promega, Madison, WI, USA) with the only modification being a 10-fold reduction in recommended reagent amounts for all steps ...following the ... See full document
8
Expression of RONIN and NANOG-associated proteins in goat parthenogenetic embryos
... The expression of a subset of transcription factors is enriched in early preimplantation embryos, which contributes to their cellular ...NANOG and its associated ... See full document
8
Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos
... research in serum supplementation during in vitro culture have focused on developmental rates and gene expression [for review see (Lonergan et ...evaluated the effects on ... See full document
8
Validation of reference genes for gene expression studies in bovine oocytes and cumulus cells derived from in vitro maturation
... level of gene expression is frequently evaluated by reverse transcription (RT) associated with the quantitative polymerase chain reaction ...allow the removal of typical ... See full document
7
Global gene expression profiling of individual human oocytes and embryos demonstrates heterogeneity in early development.
... importance of several components involved in apoptosis, cell cycle and progesterone mediated oocyte maturation pathways in the development of competent oocytes and ... See full document
14
A role for gene duplication and natural variation of gene expression in the evolution of metabolism.
... for the aliphatic glucosinolate biosynthetic pathway was a dramatic outlier in both accession datasets (Figure 5 and Table ...S3). Gene expression variation across the different ... See full document
13
In vitro production of buffalo embryos from stepwise vitrified immature oocytes
... temperature in 10% ethylene glycol (EG) in modified Dulbecco phosphate‐buffered saline (mPBS) for 5 min (PBS supplemented with 10% FCS and ...% bovine serum albumin [BSA]) and were then ... See full document
6
The constitutive expression of the V gene of Parainfluenza virus 5 affects the growth properties of bovine herpesvirus 5
... to bovine viral diarrhea virus (CRIB) were transfected with the Parainfluenza virus 5 (PIV5) V gene, previously used to block the IFN-I receptor signaling in the bovine ... See full document
3
Aging and gene expression in the primate brain.
... patterns of gene expression change in human tissues outside the brain? A recent study finding similar aging profiles of human kidney cortex and medulla regions implies that ... See full document
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