[PDF] Top 20 Expression of MdL1 and MdL2 in Pichia pastoris
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Expression of MdL1 and MdL2 in Pichia pastoris
... digestive and non-digestive lysozymes revealed adaptive properties related to the digestive function, such as a low isoelectric point (pI ), resistance to proteolytic enzymes, acidic pH optimum, reduction ... See full document
9
Applications of recombinant Pichia pastoris in the healthcare industry
... bacterial and eukaryotic organisms as production ...available in the industrial biotechnology market for a long time ...advent of recombinant DNA technol- ogy, it has become possible, however, to ... See full document
6
Transcriptional profiling of protein expression related genes of Pichia pastoris under simulated microgravity.
... P. pastoris cultured under SMG and NG conditions and reverse transcribed using a two-step strategy described previously [20] with reverse transcrip- tase PCR kit ...normalizing expression ... See full document
9
N-terminal domain of turkey pancreatic lipase is active on long chain triacylglycerols and stabilized by colipase.
... part of TPL DNA encoding the last five amino acid residues of the TPL N-terminal domain (R, D, F, P, L, W) The PCR reaction was carried out using pfu polymerase for 30 cycles with durations of 1 min ... See full document
9
Ser Huy Teh1 , Mun Yik Fong2 and Zulqarnain Mohamed 1,3
... cloning, expression and analysis of rhEPO produced in the methylotrophic yeast Pichia ...biochemical and genetic studies because of the advan- tages they offer compared to ... See full document
7
Biomarkers to evaluate the effects of temperature and methanol on recombinant Pichia pastoris
... The expression level of proteins in P. pastoris de- pends critically on growth conditions (Solà et ...2008) and ox- ygen (Verbelen et ...type-specific and varied depending on the ... See full document
10
Expression of enzymatically inactive wasp venom phospholipase A1 in Pichia pastoris.
... tubes and briefly centrifuged at 16,0006g for 20 ...removed and the cell pellet resus- pended in 200 m l RNALater solution ...removed and the cells snap-frozen in liquid nitrogen ... See full document
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Biosynthesis optimization of STEAP1 protein in Pichia pastoris X33 MUT+
... type of cancer in men over the age of 50 in Western ...are, in many cases, limited and ...specifically and effectively cancer cells. The Six Transmembrane Epithelial ... See full document
68
Production of chitin-glucan complex by Pichia pastoris
... Pichia pastoris was initially developed in 1970 by a North American oil company, named Philips Petroleum Company, for production of single-cell protein (SCP) as high-protein animal feed ... See full document
74
Expressão recombinante e caracterização funcional da β-amilase de banana produzida...
... One of the most important events that occurs during ripening of banana is the starch degradation concomitantly with the accumulation of soluble ...activity and/or specific protein detected at ... See full document
60
Eukaryotic expression system Pichia pastoris affects the lipase catalytic properties: a monolayer study.
... proteins in both prokaryotic and eukaryotic cells for both fundamental and applied research ...used in the pharmaceutical field. In this aim, interfacial and kinetic properties ... See full document
8
Avaliação da atividade antimicrobiana das defensinas recombinantes de ervilha (drr230a) e café (cd1) produzidas em Pichia pastoris
... inefficiency of chemical pesticides to control phytopathogenic fungi in agriculture and the frequent incidence of human diseases caused by bacteria which are resistant to antibiotics lead to ... See full document
112
Use of recombinant gp43 isoforms expressed in Pichia pastoris for diagnosis of paracoccidioidomycosis
... (PCM). In vitro, gp43 expression in super- natant fluids of Paracoccidioides brasiliensis cultures can be unstable, and its regulation is poorly ...secreted in the supernatants ... See full document
8
Expressão heteróloga de defensina de ervilha (pisum sativum) em pichia pastoris e avaliação da atividade biológica contra fungos de impotância agronômica
... production of economically important crops in Brazil and worldwide has been severely limited in recent years by the occurrence of diseases caused by ...Those of particular ... See full document
62
Produção em Pichia pastoris de uma quitinase de feijãodecorda com atividade antifúngica
... present in biopolymers of N-acety- -D-glucosamine, mainly chitin, a structural polysaccharide which is found in cell walls of several ...fungi. In plants, chitinases play a role as ... See full document
156
Expressão de uma quitinase de Violaceum em Pichia Pastoris: purificação e caracterização parcial da proteína recombinante.
... the expression of proteins from a variety of sources, including plants, animals and other ...group of glycosil hydrolases capable to hydrolize chitin, have already been expressed ... See full document
110
HATL5: a cell surface serine protease differentially expressed in epithelial cancers.
... produced in yeast using a Pichia Expression Kit (Invitrogen, Life Technologies, Grand Island, ...tatcgcaattgg-39 and 59-ccaattgcgataagaagtcacccgagtataga- ...resulted in a single ... See full document
11
Bao Zhen Feng, Peiqian Li
... cDNA of pclac2 en- coded a mature laccase protein containing 560 amino acids preceded by a signal peptide of 23 amino ...sequence of PCLAC2 showed high similarity with other known fungal laccases ... See full document
7
Rhipicephalus (Boophilus) microplus: expression and characterization of Bm86-CG in Pichia pastoris
... g of larvae were frozen at –20 °C and macerated using a mortar and ...volume of 8 ...amplitude and another four 10 seconds pulses at ...µm) and stored at –20 °C. The larval ... See full document
8
Protease Gene Shuffling and Expression in Pichia pastoris
... medium in a 50 mL flask at 30°C, and then 0.1 mL was taken and put into 100 mL fresh YPD medium in a 500 mL flask for incubating until an optical density of ...water and two ... See full document
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