[PDF] Top 20 MATERIALS AND METHODS Bacterial strain and cultures
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MATERIALS AND METHODS Bacterial strain and cultures
... fliC:d/j and fljB:z66, located on the chromosome and on a linear plasmid, ...FlhDC, and OmpR were constructed in this ...fliC and fljB:z66 were analyzed by qRT–PCR in the wild-type ... See full document
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MATERIALS AND METHODS Virus and cell cultures
... feeding and so was also used for poliovirus detection in the present ...repeats and this can be observed by the regression analysis applied to two different variables (virus concentration inoculated in the ... See full document
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MATERIALS AND METHODS Bacterial strain isolation and cultivation
... R and F are the amplification products using, respectively, the Reverse and Forward ...U and D are the same amplification products Undigested and Digested, ... See full document
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MATERIALS AND METHODS Cultures
... small light blue colonies with diameters of approx. 0.2 mm. The blue color of the colonies was much lighter than B. lactis, and there was no surrounding blue area, as observed for B. lactis. In the plates ... See full document
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MATERIALS AND METHODS Stock cultures
... ABSTRACT — The effects of food concentration and temperature on embryonic and postem- bryonic duration of three tropical species, Daphnia gessneri (1.5mm), Diaphanosoma sarsi (1.2mm) an[r] ... See full document
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MATERIALS AND METHODS Isolation and maintaining of cultures
... the strain selected as the best producer of cutinase was assayed with different types of cutin, extracted from apple, papaya and ...acid and 3 mL chloroform were added and vigorously shaken ... See full document
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MATERIALS AND METHODS Animal sampling and cultures
... 2008 and references therein) and Japan (Yamazaki et ...2011) and the South Brazilian State Rio Grande do Sul (Gamo & Leal-Zanchet 2004, Brac- cini & Leal-Zanchet, 2013 and in this ... See full document
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MATERIALS AND METHODS Bacterial cultures and growth media
... design and response surface analysis were possible to determine optimal operating conditions to obtain higher bacteriocin activity and total viable cell ...negative and Gram positive bacteria ... See full document
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MATERIALS AND METHODS Bacterial cultures
... world and remediation technologies should be in according to environmental sustainability ...removal and could be effective to many applications in environmental ...mangrove, and evaluate cadmium ... See full document
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MATERIALS AND METHODS Strain and growth conditions
... extension and branching at irregular intervals ...3A) and colony growth was ...treated cultures, may have resulted from biochemical disturbances caused by these compounds in one or more metabolic ... See full document
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MATERIALS AND METHODS Bacterial strains and media
... pepF::pNPTS138 strain (SP2008) showed no growth defects, with a doubling time of 3 h, equivalent to the wild type strain NA1000, and normal swimming behaviour was observed by light ...phase, ... See full document
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MATERIALS AND METHODS Yeast strain and culture condition
... washed and transferred to a fresh mineral medium containing either glucose, lactose, ethanol or potassium acetate at 1% (w/v) as carbon sources or either ammonium sulfate, glutamate, glutamine or leucine at 40 mM, ... See full document
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MATERIALS AND METHODS Bacterial isolates
... PCR. Bacterial DNA extraction: The growth of the cultures in MH agar plates in aerobiosis at 35ºC for 24h was suspended in 500 µl of 10 mM Tris-1mM EDTA (pH 8) and the turbidity was adjusted to a ... See full document
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MATERIALS AND METHODS Bacterial strains
... The application of a constant level of E. coli O157:H7 with either a similar or a higher level of P. fluorescens in the inoculum showed interesting growth patterns for both bacteria, as well as the APC. E. coli O157:H7 ... See full document
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MATERIALS AND METHODS Bacterial strains and media
... The cultures were incubated for 14 days, when cells show the elongated morphology characteristic of late stationary phase (29), and then plated in rich ... See full document
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MATERIALS AND METHODS Yeast strain and medium
... Furthermore, “synchrony in S. cerevisiae has been reported several times to start after small substrate pulses, shifts of dilution rate, pH, aeration or other disturbances of asynchronous cultures” (13). Presently ... See full document
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MATERIALS AND METHODS Bacterial isolates
... treated and untreated MAP ...USA) and homogenised with ...mixed and centrifuged (10,000 ×g for 10 min at ...tube and 350 l of RLT buffer was added (RNeasy kit, Qiagen, Hilden, ...buffer ... See full document
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MATERIALS AND METHODS Bacterial strains
... aureus and Salmonella sp. inoculated crab meat, we used 600 and 100 µg/mL of nisin wash solution, respectively and pH 10 sodium bicarbonate ...concentration and pH level was based on their ... See full document
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MATERIALS AND METHODS Bacterial strains
... Brazil and was kindly donated by Prof. Marcelo Brocchi. The reference strain was obtained directly from American Type Culture Collection (ATCC), ...The bacterial strains were kept at - 70ºC in tubes ... See full document
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MATERIALS AND METHODS Bacterial strains
... overnight cultures of ...inoculum and antibiotics were dispensed into each well of the microtitre plate ...plates and incubating for 18–24 ... See full document
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