[PDF] Top 20 MATERIALS AND METHODS Isolation and maintaining of cultures
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MATERIALS AND METHODS Isolation and maintaining of cultures
... cutinase and lipase belong to the esterase group of enzymes (EC ...capable of catalyzing the hydrolysis of ester ...hydrolysis of cutin, an insoluble biopolyester which is the ... See full document
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MATERIALS AND METHODS Isolation of thermophilic fungi
... pectinase and PG activities were determined using pectin and polygalactouronic acid as substrates ...amounts of substrate (1%) prepared in citrate buffer (0.05 M pH4.4) and suitably diluted ... See full document
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MATERIALS AND METHODS Isolation of keratin degrader
... approach and Phylogenetic analysis The Genomic DNA of the test strain Streptomyces IF 5 was isolated using the procedure of Hopwood et ...(rDNA) of the Streptomyces strain. The primers used ... See full document
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MATERIALS AND METHODS Isolation and screening of hypercellulolytic and xylanolytic microorganisms
... residue of forest species Toona ciliata, Celtris australis, Cedrus deodara and Pinus roxburghii was selected as substrate for biodegradation study due to its easy availability and wide use in ... See full document
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MATERIALS AND METHODS Isolation of fungal strains
... raw materials, cost intensive hydrolysis processes are required to obtain fermentable ...Hydrolysis of cellulose with diluted acid occurs at high-temperature, whereas hydrolysis with either concentrated ... See full document
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MATERIALS AND METHODS Isolation and identification of bacterial strains
... East of Tunisia (33 0 44 N – 10 0 46 E). Bacteria-free cysts and nauplii were obtained via decapsulation as described by Sorgeloos et ...filtered and autoclaved sea water (FASW) over a 50 m sterile ... See full document
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MATERIALS AND METHODS Isolation of an aromatic-degrading bacterium
... activity of catechol 2,3-dioxygenase in that case was rather small ...activity of intradiol catechol dioxygenase (Table ...kinds of dioxygenases including protocatechuate 4,5- dioxygenase for ... See full document
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MATERIALS AND METHODS Isolation and inoculum preparation
... objectives of this study were to determine the effects of leaf position, surface, and the need for entry sites, such as wounds, other pathogen lesions or natural openings, to promote infection ... See full document
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MATERIALS AND METHODS Isolation of the organism
... plates and the cells scraped off using a sterile Pasteur ...nm of at least 0.1 and the cultures were incubated at temperatures starting at 50ºC and going up to 65ºC, with vigorous ... See full document
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MATERIALS AND METHODS Isolation of staphylococci
... web and ascitic fluid of patients from May 2006 to March 2007 in Nemazee hospital, affiliated with Shiraz University of Medical Sciences, Shiraz, ...coagulase and DNAse activities using ... See full document
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MATERIALS AND METHODS Isolation and characterization of Gram-positive and Gram- negative bacterial strains
... dihydrolase and urease production, esculin and gelatin hydrolysis, -galactosidase production, and assimilation of different substrates such as D- glucose, L-arabinose, D-mannose, D-mannitol, ... See full document
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MATERIALS AND METHODS Isolation and maintenance of microbes
... solution and 500 µl of 0.36 % casein and 2.0ml of 100mM acetate buffer (pH ...C and stopped with 3 ml of 5 % trichloroacetic acid ...precipitate and known volume of ... See full document
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MATERIALS AND METHODS Isolation of microorganism
... number of fungi, including Aspergillus, Rhizopus, Fusarium, Candida, Penicillium, Thermomucor, basidiomycete Fomitopsis and Thermomyces (Balkan et ...Majority of the studies on fungal amylases are ... See full document
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MATERIALS AND METHODS Isolation and biochemical characterisation of L. reuteri LPB P01-001
... tract of wild swine and was characterised by biochemical testing and sequencing of gene 16S ...simple and low-cost culture medium based on cane sugar (2.5% p/v) and yeast extract ... See full document
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MATERIALS AND METHODS Isolation and screening of microorganisms
... preparation of instantaneous tea, action on tea polyphenols, in the production of gallic acid (17), beer chillproofing and wine ...one of the richest sources of vitamin C and has ... See full document
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MATERIALS AND METHODS Collection of samples, Isolation and Identification of
... virulence and cytotoxicity of Aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in Chennai, Tamil Nadu, ...fish and shrimp samples, ...protease and ... See full document
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MATERIALS AND METHODS Enrichment, Isolation and Screening of Cellulases Producing Thermophilic Bacteria
... mixed and incubated at 50 0 C for 72 ...consisting of basal medium as above and the plates were incubated at 50 0 ...Out of the seven isolates, two isolates having colonies with high ... See full document
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MATERIALS AND METHODS Bacterial cultures and growth media
... bacteriocin and viable cells production were identified by the probability levels above 95% (P< ...method of steepest ascent path was performed along with the factorial design so that the optimum would ... See full document
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MATERIALS AND METHODS Bacterial strain isolation and cultivation
... determination of the molecular mass of this type II RE was estimated by polyacrylamide gel electrophoresis ...presence of sodium-dodecyl sulfate (SDS) carried out by the method of Laemmli ... See full document
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MATERIALS AND METHODS Bacterial isolation and identification
... haemolysins and enterotoxins, have been identified to cause disease in Aeromonas ...ECPs of strain PY50 was lethal to Chinese longsnout catfish and the LD 50 was ...fish, and the results were ... See full document
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