[PDF] Top 20 MATERIALS AND METHODS Isolation of an aromatic-degrading bacterium
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MATERIALS AND METHODS Isolation of an aromatic-degrading bacterium
... activity of catechol 2,3-dioxygenase in that case was rather small ...activity of intradiol catechol dioxygenase (Table ...kinds of dioxygenases including protocatechuate 4,5- dioxygenase for ... See full document
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MATERIALS AND METHODS Isolation of the organism
... use of these microbes and their enzymes for biotechnological applications ...majority of the investigated thermophilic bacteria belong to the genus Bacillus and they have been isolated from ... See full document
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MATERIALS AND METHODS Isolation of thermophilic fungi
... pulp. An acidic pH of 4.0-5.0 was found to support high pectinase and PG ...temperatures and low pH. Further work on purification and characterization of pectinase from ... See full document
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MATERIALS AND METHODS Isolation and screening of hypercellulolytic and xylanolytic microorganisms
... With an inevitable depletion of the world’s energy supply, there has been an increasing worldwide interest in alternative sources of ...importance of alternative energy source has ... See full document
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MATERIALS AND METHODS Isolation of keratin degrader
... cooking, and many scientists have worked to find the nutritional value of the feather ...is an important source for animal production, it represents low digestibility ...existence of ... See full document
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MATERIALS AND METHODS Collection of samples and source of the microbial inocula
... absence of methanogenic organisms, the degradation pathway of peptide and amino acids is linked to fast growing organisms of the genus ...example of an asaccharolytic amino acid ... See full document
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MATERIALS AND METHODS Isolation of fungal strains
... identified and studied from this fungus. A cellobiohydrolase I (cbhI) and a beta-glucosidase 1 (bgl1) gene of ...component and can therefore be considered a xyloglucan- specific endo-beta-1, ... See full document
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MATERIALS AND METHODS Bacterial strain isolation and cultivation
... at an elution volume. In addition, the determination of the molecular mass of this type II RE was estimated by polyacrylamide gel electrophoresis ...presence of sodium-dodecyl sulfate (SDS) ... See full document
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MATERIALS AND METHODS Isolation and maintaining of cultures
... cutinase and lipase belong to the esterase group of enzymes (EC ...capable of catalyzing the hydrolysis of ester ...hydrolysis of cutin, an insoluble biopolyester which is the ... See full document
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MATERIALS AND METHODS Isolation and characterization of Gram-positive and Gram- negative bacterial strains
... comprises of three subunits: an RND transporter, a membrane fusion protein and an outer membrane ...domains and constitute a superfamily of transporter proteins with a variety ... See full document
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MATERIALS AND METHODS Isolation and screening of microorganisms
... Optimization of process parameters using PR as substrate Optimum tannase production ...U/mL) and gallic acid (52 g/mL) were achieved after 72 h with 4 % (w/v) substrate (PR), at 37 0 C, pH ...with an ... See full document
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MATERIALS AND METHODS Collection of samples, Isolation and Identification of
... pattern of the strains had no direct relation to the cytotoxic activities as observed in vitro but might play an important role in the pathogenesis of the organism in ...irrespective of ... See full document
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MATERIALS AND METHODS Bacterial isolation and identification
... considered an appropriate tool for the reconstruction of evolutionary history and phylogenetic relationships of bacterial genera and it is universally used ...composed of a very ... See full document
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MATERIALS AND METHODS Screening and isolation conditions
... late of log phase, and harvested by centrifugation at 8,000 X g for 10min at 4ºC, suspended in sodium phosphate buffer ...again, and sonificated at ...12,000g and 4ºC for 20min. The cell free ... See full document
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MATERIALS AND METHODS Isolation and biochemical characterisation of L. reuteri LPB P01-001
... tract of wild swine and was characterised by biochemical testing and sequencing of gene 16S ...simple and low-cost culture medium based on cane sugar (2.5% p/v) and yeast extract ... See full document
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MATERIALS AND METHODS Antagonistic Bacterium
... ratio and then combined with ...with an equal volume of autoclaved ...sheet and air dried in a laminar air-flow cabinet at 24ºC for 1 hour to form a slightly moistened powder (15% moisture ... See full document
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MATERIALS AND METHODS Isolation of staphylococci
... result of S. aureus acquiring the mecA gene, which encodes for an altered penicillin binding protein gene (PBP2a) ...outbreaks of MRSA infections, and subsequently MRSA clones spread to ... See full document
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MATERIALS AND METHODS Primary isolation
... annually and their use as raw materials in technological processes for obtaining products of high added value is gaining ...kinds of agro-industrial wastes have been reported for the ... See full document
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MATERIALS AND METHODS Isolation of microorganism
... presence of readily available substrates has been noted to influence the biosynthesis of many extracellular enzymes via catabolite repression mechanism (Teodoro and Martins, ...use of glucose ... See full document
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MATERIALS AND METHODS Enrichment, Isolation and Screening of Cellulases Producing Thermophilic Bacteria
... aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) ... See full document
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