[PDF] Top 20 MATERIALS AND METHODS Isolation of the organism
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MATERIALS AND METHODS Isolation of the organism
... aiming the use of these microbes and their enzymes for biotechnological applications ...(1,5,6,8,12,20,22). The majority of the investigated thermophilic bacteria belong to ... See full document
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MATERIALS AND METHODS Screening and isolation conditions
... determination and phylogenetic analysis Isolation of genomic DNA, PCR amplification of the 16S rDNA and sequencing of the purified PCR products were carried out as ... See full document
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MATERIALS AND METHODS Isolation and maintenance of microbes
... µl) of the culture supernatant was incubated with 400 µl of 100mM sodium citrate buffer (pH ...min., the glucose released was measured by the dinitrosalicylic acid (DNSA) method ... See full document
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MATERIALS AND METHODS Isolation and inoculum preparation
... affects the new shoots of young trees of spotted gums and some other Corymbia ...leaves and stems within five ...10. The disease cycle can, therefore, be completed within 10 days ... See full document
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MATERIALS AND METHODS Bacterial isolation and identification
... warm-water and carnivorous freshwater aquaculture species and is high valued because of its good taste in ...outbreaks of ulcerative syndrome and mass mortalities occurred in China ... See full document
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MATERIALS AND METHODS Isolation and screening of microorganisms
... Optimization of process parameters using PR as substrate Optimum tannase production ...U/mL) and gallic acid (52 g/mL) were achieved after 72 h with 4 % (w/v) substrate (PR), at 37 0 C, pH ...size of ... See full document
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MATERIALS AND METHODS Organism, maintenance and culture condition
... activity of ß-glucosidase was observed on sucrose. Various cultural parameters of cultivation of ...production of ß-glucosidase were ...hours of cultivation at 30°C. Addition of ... See full document
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MATERIALS AND METHODS Organism, maintenance and culture conditions
... extracellular and intracellular fructosyltransferase after 4 and 3 days of fermentation, respectively ...in the cultivation medium reached zero after four days of ...biomass and ... See full document
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MATERIALS AND METHODS Isolation and maintaining of cultures
... cutinase and lipase belong to the esterase group of enzymes (EC ...capable of catalyzing the hydrolysis of ester ...catalyzes the hydrolysis of cutin, an insoluble ... See full document
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MATERIALS AND METHODS Isolation and identification of bacterial strains
... evaluate the effect of the bacterial strains on Artemia culture, twelve challenge tests were ...performed. The first test was Artemia alone (Axe), the second was Artemia with Candida ... See full document
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MATERIALS AND METHOD Source of organism and inoculants’ preparation
... in the production of many diseases, particularly those characterized by soft-rotting of ...liquefaction of the pectic substances that hold plant cells together and in weakening ... See full document
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MATERIALS AND METHODS Enrichment, Isolation and Screening of Cellulases Producing Thermophilic Bacteria
... by the presence of material that has already been hydrolyzed (feedback ...(Suto and Tomita ...stearothermophilus and B. licheniformis (Hala and Priest 1994). One of the ... See full document
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MATERIALS AND METHODS Isolation and biochemical characterisation of L. reuteri LPB P01-001
... from the gastrointestinal tract of wild swine and was characterised by biochemical testing and sequencing of gene 16S ...simple and low-cost culture medium based on cane sugar ... See full document
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MATERIALS AND METHODS Isolation and screening of hypercellulolytic and xylanolytic microorganisms
... transformation and shown in subscript (Table ...SSF of forest biomass. The significant increase in biodegradation of forest lignocellulosics by SSF with modified BSM was due to the ... See full document
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MATERIALS AND METHODS Materials
... Determination of minimum inhibitory concentration (MIC) From Table 2 it can be observed that all microorganisms were susceptible to the action of oregano essential oil, with a variation in the ... See full document
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MATERIALS AND METHODS Isolation of an aromatic-degrading bacterium
... sludge and was found to utilize different aromatic substrates as sole carbon and energy ...On the basis of morphological and physiochemical characteristics and 16S rRNA gene ... See full document
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MATERIALS AND METHODS Isolation of fungal strains
... CMCase and xylanase activities were determined by the spectrophotometric method described by Miller et ...substrate, and dinitrosalicylic acid (DNS) as a coupling reagent. Crude enzyme and ... See full document
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MATERIALS AND METHODS Collection of samples, Isolation and Identification of
... determine the virulence and cytotoxicity of Aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in Chennai, Tamil Nadu, ...fish and shrimp samples, ... See full document
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MATERIALS AND METHODS Bacterial strain isolation and cultivation
... NaCl, and 0.02 mM phenyl-methyl-sulfonyl-fluoride (PMSF). The cell suspension was incubated for 30 min in the presence of lysozyme ...pulses of 30 sec each) at 4ºC, and ... See full document
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The comparison of the structure and microhardness of the tool steel C90 and HS 6-5-2 remelted with the electric arc
... group of materials used for the production, not only tools, but also machine ele- ments, that need to have the increased strength, for example the high-speed steels are used on ... See full document
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