[PDF] Top 20 The peroxisomal protein import pathway: a functional study of the N-terminal half of PEX5
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The peroxisomal protein import pathway: a functional study of the N-terminal half of PEX5
... domínio N-terminal pobre em estrutura secundária/terciária que contém repetições de pentapéptidos aromáticos e uma cisteína evolucionariamente conservada, e ii) um domínio C-terminal conservado e ... See full document
86
The N terminus of the peroxisomal cycling receptor, Pex5p, is required for redirecting the peroxisome-associated peroxin back to the cytosol
... to study the process of association (import)/dissociation (export) of Pex5p with and from the peroxisomal ...consists of incubating 35 S-labeled Pex5p with a ... See full document
7
A cell-free organelle-based in vitro system for studying the peroxisomal protein import machinery
... at the peroxisomal membrane docking/translocation machinery (DTM; Stage 1b) where it becomes inserted (Stage 2) resulting in the release of the cargo protein into the ... See full document
37
Characterization of the mechanisms of protein translocation across the mammalian peroxisomal membrane
... soluble PEX5 (stage 0) binds to cargo proteins in the cytosol yielding stage 1a ...changes the conformation of PEX5 because the receptor only engages the next steps ... See full document
101
Factors involved in ubiquitination and deubiquitination of PEX5, the peroxisomal shuttling receptor
... to the PTS1, which remains intact upon import, the PTS2 is generally cleaved in the peroxisomal matrix by a serine protease (Kurochkin et ...all peroxisomal matrix proteins are ... See full document
19
Mapping of the cargo protein membrane translocation step into the PEX5 cycling pathway.
... no N-terminal das proteínas [7, ...pela PEX5, o receptor peroxissomal cíclico ...da PEX5 mas as PTS2 requerem a acção da proteína adaptadora PEX7, que medeia a interacção com a PEX5 ... See full document
86
The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo
... Radiolabeled PEX5(1-197;C11A) was subjected to PNS-based in vitro assay in the absence (lane 1) or presence (lane 2) of 10 µM ...PAGE/autoradiography. The autoradiograph (upper panel) and ... See full document
40
The peroxisomal protein import machinery displays a preference for monomeric substrates
... each of these species in translation reactions is time-depen- dent: synthesis reactions performed for a short period of time yielded essentially monomeric catalase; longer incubations led to the ... See full document
13
The N-terminal half of the peroxisomal cycling receptor Pex5p is a natively unfolded domain
... capable of interacting directly with PTS1-containing proteins ...its N-terminal half displays the properties of a lever capable of coupling the force generated ... See full document
12
PEX5, the Shuttling Import Receptor for Peroxisomal Matrix Proteins, Is a Redox-Sensitive Protein
... by the shuttling receptor PEX5 (9), (ii) this receptor accumulates at the peroxisomal membrane in aging cells (7), and (iii) the export of PEX5 from the ... See full document
15
Ubiquitin in the peroxisomal protein import pathway
... ATPases of the REM, PEX1 and PEX6. The mechanistic details of how monoubiquitinated PEX5 and PEX5-related proteins are recognized by the REM are not entirely ...principle, ... See full document
17
The peroxisomal matrix protein import machinery - a PEX5 - centered perspective
... that PEX5 functions as a holdase-like protein. The data behind this concept comes from the finding that PEX5 binds monomers of human catalase (a tetrameric protein in its ... See full document
115
Ubiquination and deubiquitination in the mammalian pex5-mediated peroxisomal protein import pathway.
... identity of the E2(s) mediating this ubiquitination event was an immediate ...cerevisiae the occurrence of PEX4-dependent monoubiquitination at the conserved N-terminal ... See full document
91
Synapse associated protein 102 (SAP102) binds the C-terminal part of the scaffolding protein neurobeachin.
... scaffold protein abundant in the brain, where it is highly expressed during ...paralysis of the ...for the functioning of central synapses, where it is suggested to play a role ... See full document
12
Functional characterisation of the novel WNK2 protein kinase
... identify the relevant experimental models, WNK2 expression was further analysed by RT‐PCR in various human cancer cell ...and protein were detected in HT29 (colon), HeLa (cervix carcinoma) and HEK293 (human ... See full document
213
Unbounded solutions for functional problems on the half-line
... on the half-line arise naturally in the study of radially symmetric solutions of nonlinear elliptic equations, and many works have been done in this area; see ...[1]. The ... See full document
8
Impact of hospitalization on the functional capacity of the elderly: a cohort study
... verify the trajectory of the functional capacity of elderly persons hospitalized due to clinical conditions in a university ...cohort study was conducted between 2015 and ...to ... See full document
9
Study of the TRIB2 protein network
... type of cancer that arises from the transformation of melanocytes, the pigment-producing cells of the human ...is the deadliest form of skin cancer for which ... See full document
95
Impact of hospitalization on the functional capacity of the elderly: A cohort study
... verify the trajectory of the functional capacity of elderly persons hospitalized due to clinical conditions in a university ...cohort study was conducted between 2015 and ...to ... See full document
9
The PEX5/PEX7 - mediated import of PTS2 - containing matrix proteins: mechanistics insights on PEX7 translocation and recycling steps
... comprises the Zellweger syndrome (ZS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD) and rhizomelic chondrodysplasia punctata (RCDP) type ...After the discovery that mutations in ... See full document
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