[PDF] Top 20 Phenotypic and genotypic characteristics of shiga toxin-producing Escherichia coli strains isolated from children in São Paulo, Brazil

... 505 children with bloody and non-bloody acute diarrhea (case patients) and 505 age-matched chil- dren (1-4 years old) without any gastrointestinal signs or symptoms during the 30 days before ... See full document

... E. coli strains studied belonged to the collection of Instituto Adolfo Lutz, a Central Public Health Laboratory in Sa˜o Paulo, Brazil, and national center for ...E. ... See full document

... Bacterial strains. A total of 76 E. coli strains isolated in Sa˜o Paulo, Brazil, during the period from 1976 to 2003 were included in this ...STEC ... See full document

... prevalence of Shiga toxin-producing Escherichia coli (STEC) in ground beef collected in two cities located in the State of São Paulo, ...total ... See full document

... properties and characteristics of Shiga toxin-producing Escherichia coli in São Paulo, Brazil, from 1976 through ... See full document

... occurrence of E. coli harboring stx1 and/or stx2 virulence genes in the raw milk ...were from Invitrogen (Carlsbad, CA, USA), and the molecular biology reagents were from ... See full document

... ETEC strains are important pathogens in Sa˜o Paulo, Brazil, and are responsible for 7 to 20% of cases of infantile diarrheal (8, ...occur in 43 to 29% of the ... See full document

... abattoir in São Paulo State, in southwestern Brazil, between March 2008 and August ...were from carcasses cattle raised at pastures. Sampling of 150 feedlot cattle was ... See full document

... Stx1 and Stx2 were purchased from Tufts University School of Medicine, Boston, MA, ...bought from GE Healthcare, UK. Ni-NTA beads were purchased from Qiagen (Hilden, ...(NsiI/SalI ... See full document

... were in respect to the frequency of the categories and the kind of ...categories in their studies were a-EPEC, EAEC, and EHEC while in our studies the most frequent ones ... See full document

... Analysis of the interaction of selected O26 strains with Caco-2 intes- tinal cells and human duodenal ...mucosa. In vitro cell culture adhesion assays were performed with polarized ... See full document

... E. coli strains carrying eae but lacking the EAF plasmid probe sequence and the Shiga toxins genes (stx) have been detected in different studies ...Many of these strains ... See full document

... tion of biofilm formation by the prototype tEPEC E2348/ 69 (O127:H6) ...BFP, and EspA, were expressed during the initial stages of biofilm ...mation of a robust biofilm on an abiotic surface ... See full document

... found, and among the identified six STEC strains, four carried eaeA and stx1, two carried stx1 alone, while none of the strains carried ...reports in which stx1 genes were more ... See full document

... E. coli strains that were identified as causative agents of intestinal disorders have been defined as a specific enteropathogenic pathotype ...These strains were initially diagnosed with ... See full document

... quantification of biofilm formation was performed in 96-well polystyrene microtiter plates (Corning Costar Corporation, Cambridge, MA) by the method of Uhlich et ...performed in triplicate ... See full document

... Committee of Re- search (reference number 129/06) in the Health Science of Center at the Universidade Federal do Espírito Santo, ...read and signed by the parents or guardians of each ... See full document

... Bacterial strains. A panel of 65 O113:H21 strains was used in the present study: 12 strains from Argentina, mostly from ground beef and cattle but also included a ... See full document

... Enterotoxigenic Escherichia coli (ETEC) is recognized as the main cause of bacterial diarrhoea among children in Asia, Africa and Latin America but less investigated in ... See full document

... STEC strains isolated from humans and animals have acquired antibiotic resistance genes almost twenty years ago (Farina et ...1 and 2 integrons are strongly associated with ... See full document