[PDF] Top 20 Scalable culture strategies for the expansion of patient-derived cancer stem cell lines
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Scalable culture strategies for the expansion of patient-derived cancer stem cell lines
... Cancer stem cells (CSCs) have recently raised great interest as a promising biological system for designing e ffective cancer ...therapies. The scarcity of CSCs in vivo and the ... See full document
8
Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems.
... static cell culture systems that cannot pro- vide a sufficient number of cells for downstream applications, presenting low cell yields and inherent variability of the ... See full document
19
Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies
... in the future, an aliquot of patients’ blood could be immunophenotyped by flow cytometry to assess the expression of the inhibitory and/or stimulatory co-receptors present at the ... See full document
13
Morphology and morphometry of feline bone marrow-derived mesenchymal stem cells in culture
... is the traditional source of MSC, and bone marrow-derived mesenchymal stem cells (BM- -MSCs) have the advantages of being easily and relatively noninvasively obtained and have a ... See full document
8
MiRNA Profiles in Lymphoblastoid Cell Lines of Finnish Prostate Cancer Families.
... for the outcome of solid tumours and leukaemias, but the contribution of altered miRNA expression levels to ge- netic cancer susceptibility is not ...known. The transcriptional ... See full document
17
Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431
... with the appearance of small bodies. The nature and the differential features occurred in the cancer cells clearly defined the cellular morphological characteristics, ... See full document
7
Strategies to Improve Homing of Stem Cells to achieve better Efficacy in Stem Cell Therapy
... preconditioning of the cells with hypoxia, cytokine and growth factor priming of the cells, genetic modifications, coating of cell surface with antibodies, glycoengineering, ... See full document
1
Scalable passaging of adherent human pluripotent stem cells.
... 25 cell passages, were dissociated using the hypertonic citrate .... The cell suspension was injected intramuscularly into the right hind leg flank of six week old SCID/ Beige ... See full document
9
Mutation detection in tumor-derived cell free DNA anticipates progression in a patient with metastatic colorectal cancer
... colorectal cancer (KRAS, and TP53) exhibited robustly elevated allele frequencies in the preoperative plasma ...sample. The allele frequencies of both mutations decreased sharply after ... See full document
6
Exploring the role of metabolism in the differentiation and maturation of human pluripotent stem cell derived cardiomyocytes
... result of lipotoxicity, lipid accumulation was quantified through the use of a commercial kit that relies on Nile Red, a fluorescent dye specific for lipid droplets 74 ...most of fatty acid ... See full document
52
Primary cilia: the chemical antenna regulating human adipose-derived stem cell osteogenesis.
... adipose-derived stem cells (hASC) are obtained from waste adipose tissue derived from anonymous patients undergoing elective abdominoplasty surgeries (IRB exemption protocol #10- 0201), at University ... See full document
11
An engineered approach to stem cell culture: automating the decision process for real-time adaptive subculture of stem cells.
... implementing the confluency measurement and predictions on a local machine, a server (cloud computing cluster) was utilized so that multiple experiments can be conducted in parallel from different microscope ... See full document
12
Establishment of new murine embryonic stem cell lines for the generation of mouse models of human genetic diseases
... tomes, the next challenge is to determine gene ...for the study of gene function in vivo is the generation of ...In the case of human genes, mouse models are mostly used ... See full document
8
Molecular mechanisms underlying the anticancer activity of lactoferrin in highly metastatic cancer cell lines
... Furthermore, the anticancer activity of Lf also stems from its anti-metastatic potential ...production of IL-18 and activation of NK and T cells to enhance immunity is a consequence of ... See full document
86
Evaluating the effectiveness of an inactivated vaccine from Anaplasma marginale derived from tick cell culture
... reduce the occurrences of clinical cases and animal mortality due to acute rickettsemia and subsequently diminished blood packed cell volume ...areas of enzootic instability, administration ... See full document
6
Karyotype characterization of in vivo- and in vitro-derived porcine parthenogenetic cell lines.
... oviducts of four gilts (195, 128, 165 and 197 embryos transferred). Three of the gilts developed pregnancies and a total of 50 fetuses were collected on day ...30. The body size ... See full document
14
Isolation, characterization and Ex-Vivo expansion of human synovial tissue derived-mesenchymal stem/stromal cells
... at the isolation and expansion of synovium-derived mesenchymal stem cells, extracted by direct biopsy, shaver blade filtrate residues or synovial fluid, while comparing their ... See full document
97
Adipose Tissue-Derived Stem Cell Treatment Prevents Renal Disease Progression
... return of renal ...check the long-term consequences of AKI ...effect of treatment with ASCs, where the initial immunomodulation was still persistent with enhanced expression of ... See full document
15
Prognostic value of stem cell quantification in stage II colon cancer.
... identify stem cell ...following the Dako Real En Vision rabbit/mouse method (Dako, ...control of pattern expression and signal specificity, we used fetal human brain tissue sections including ... See full document
8
Stem cell-derived retinal pigment epithelium models of lysosomal dysfunction in disease
... Pathogenesis of AMD is regarded as the result of a combination of metabolic, functional, genetic and environmental factors (Hamdi & Kenney ...On the one hand, with aging, the ... See full document
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