[PDF] Top 20 A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
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A specific polymerase chain reaction method to identify Stenotrophomonas maltophilia
... due to the higher variability in this region among species of the Stenotrophomonas genus compared to the 16S rRNA gene, as determined using CLUSTALW software (data not ...S. maltophilia ... See full document
2
Mycobacteria mobility shift assay: a method for the rapid identification of Mycobacterium tuberculosis and nontuberculous mycobacteria
... phenotypic method is time consum- ing and may last up to 60 ...affordable, specific and easy-to-perform identification methods are ...a polymerase chain reaction-based ... See full document
6
Polymerase chain reaction – PCR
... rise to any amplification of the alpha toxin ...amplification to show that alpha toxin gene is specific to ...diagnostic method for confirmation of ...alternative to the ... See full document
7
EVALUATION OF GBV-C HGV VIREMIA IN HIV-INFECTED WOMEN
... quantitative polymerase chain reaction assay to evaluate the presence of GBV-C/HGV ...quantitative polymerase chain reaction method developed is appropriate for the ... See full document
5
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
... order to validate a fast and efficient ...was to compare the culture technique (es- tablished as the gold standard) with the molecular method of polymerase chain reaction (PCR) ... See full document
5
Rev. Soc. Bras. Med. Trop. vol.48 número2
... was to identify a rapid and simple phenotypic method for extended-spectrum β-lactamase (ESBL) detection in Enterobacter ...compared to multiplex polymerase chain reaction ... See full document
4
Use of multiplex allele-specific polymerase chain reaction (MAS-PCR) to detect multidrug-resistant tuberculosis in Panama.
... (DR) to Mycobacterium tuberculosis has not been studied previously in Central America, the place of origin of many immigrants to the United ...leading to increased MDR-TB transmission in the ... See full document
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Detection of genomic mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis isolates using polymerase chain reaction and multiplex allele-specific polymerase chain reaction
... MAS-PCR method was used for simultaneous detection of the katG gene codon 315 and rpoB gene codons 516, 526, and 531, and ...located to pair with the base of the related codons where most point mutations ... See full document
6
Identification of bovine material in porcine spray-dried blood derivatives using the Polymerase Chain Reaction technique
... bovine specific PCR test in SDARC samples is illustrated in figure ...unable to identify the presence of bovine contamination in the red cells fraction due to the lack of ...the ... See full document
7
Polymerase Chain Reaction (PCR) Identification of Terverticillate
... extraction method and then used for PCR amplification with the internal transcribed spacer 1 (ITS1) and ITS4 universal fungal specific ...solution to taxonomy and improve food safety and to ... See full document
5
Brazilian ground beef authentication by multiplex polymerase chain reaction
... Thus, to identify possible artifacts related to multiplex PCR, conventional PCR using the forward primer and a single reverse primer were ...was specific for either cattle or buffalo ...PCR ... See full document
7
A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus
... diagnostic method due to the lack of re- agents and requirement for expertise in tech- niques such as immunodiffusion and immunoelectroosmophoresis ...developed to differentiate HBV sub- types by PCR ... See full document
5
Modelling of Serpentine Continuous Flow Polymerase Chain Reaction Microfluidics
... A tetra-mesh grid from 500K to 3M nodes was used to avoid mesh independence on the solution regime. The geometry is boxed within 17 22 0.005mm. This chip model geometry has similar design with the one ... See full document
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MATERIALS AND METHODS Field samples
... For pre-enrichment, 25 g of sample was inoculated into 250 mL of 1% buffered peptone water (BPW) and incubated at 37ºC for 18-24 h. For enrichment, 1 mL of BPW culture was transferred to 9 mL of ... See full document
5
Sensitivity of diagnostic methods for Mansonella ozzardi microfilariae detection in the Brazilian Amazon Region
... respectively, to amplify the ...second reaction incorporated the products of the first reaction together with the primers FL1-F (5’-TTC- CGTAGGTGAACCTGC-3’) and Di660R (5’-ACCCT- CAACCAGACGTAC-3’) in ... See full document
5
Toxic cyanobacteria in reservoirs in northeastern Brazil: detection using a molecular method
... the polymerase chain reaction (PCR) method, eleven environmental samples with cyanobacteria from seven reservoirs were used to determine the presence of the gene involved in microcystin ... See full document
6
Abnormal apoptosis of trophoblastic cells is related to the up-regulation of CYP11A gene in placenta of preeclampsia patients.
... Placenta tissue or HTR-8/SVneo cells were lysed in RIPA buffer (150 mM sodium chloride; 1.0% NP-40 and Triton X-100; 0.5% sodium deoxycholate; 0.1% SDS; 50 mM Tris, PH8.0) Whole cell protein was extracted by centrifuge ... See full document
8
Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis
... added to preputial and/or cervical mucus and abomasum contents suggesting that the possible inhibitors of the PCR reaction present in the inoculated transport medium had no affect on the final result of the ... See full document
5
Farmacologia dos antibióticos aminoglicosídeos
... Estes antibióticos não apresentam, ainda, atividade contra Burkholderia cepacia nem Stenotrophomonas maltophilia e embora mais de 95% de estafilococos coagulase negativos e de Staphylo[r] ... See full document
72
21-Hydroxylase deficiency in Brazil
... subject to unequal crossover events and gene conversions ...was to determine the frequency of large mutations by Southern blotting analy- sis and the frequency of point mutations by allele-specific ... See full document
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