[PDF] Top 20 Total RNA isolation and cDNA synthesis
Has 10000 "Total RNA isolation and cDNA synthesis" found on our website. Below are the top 20 most common "Total RNA isolation and cDNA synthesis".
Total RNA isolation and cDNA synthesis
... analysis and quantification of mRNAs transcribed in studies of water deficit tolerance has contributed to the understanding of biochemical and molecular processes of plants under stress, therefore, such ... See full document
11
ALT-C, A DISINTEGRIN-LIKE CYS-RICH PROTEIN FROM Bothrops alternatus, INCREASES SKELETAL MYOBLAST VIABILITY Mesquita-Ferrari RA (1), de Moraes CK (2), Micocci KC (2), Selistre-de-Araújo HS (2)
... signaling and neutrophil ...concentrations and incubation periods were followed by total RNA ...isolation. cDNA synthesis and real time polymerase chain reaction ... See full document
15
The non-palindromic adaptor-PCR method for the identification of the T-cell receptor genes of an interferon-gamma-secreting T-cell hybridomaspecific for trans-sialidase, an immunodominant Trypanosoma cruzi antigen
... clonotypical, isolation of their genes requires reverse transcriptase- assisted PCR using primers specific for each different Vα or Vß genes or by the screening of cDNA libraries generated from RNA ... See full document
10
Clonagem e avaliação da expressão gênica do sbGnRH em machos juvenis e adultos de linguado, Paralichthys orbignyanus.
... O RNA total foi isolado usando-se o reagente TRIzol ® (Invitrogen ™ , EUA), e o tratamento com DNase foi realizado com o kit DNA-free ® (Ambion ™ , EUA) seguindo as instruções do ...de RNA foram ... See full document
8
Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
... - Total RNA was extracted with Trizol re- agent according to manufacturer's protocol from groups of 20 fully engorged females at 2, 6, 12, 24, 48, 72, 96, and 168 h after blood feeding, or 72 h after ... See full document
8
Analysis of the workers head transcriptome of the Asian subterranean termite, Coptotermes gestroi
... Total RNA was extracted from the whole body of different castes and developmental stages (larvae, soldiers, alates, queens and workers) using QIAGEN RNAeasy Mini ...Kit. RNA samples (5 ... See full document
9
Expression and cellular localization of molecules of the gp82 family in Trypanosoma cruzi metacyclic trypomastigotes
... collected, and an equal volume of isopropyl alcohol was added to precipitate the total ...following: total T. cruzi RNA; deoxynucleoside triphosphate mix; avian myeloblastosis virus reverse ... See full document
7
Altered expression of cyclin A 1 in muscle of patients with facioscapulohumeral muscle dystrophy (FSHD-1).
... Total RNA was isolated with the Qiagen RNeasy mini kit including the RNase-Free DNAse set (Qiagen, Hilden, Germany) according to the instruction of the ...manufacturer. RNA (1 mg) was reverse ... See full document
9
WDR55 is a nucleolar modulator of ribosomal RNA synthesis, cell cycle progression, and teleost organ development.
... p53 and controls cell cycle [36,37], was significantly increased, while that of WDR55 mRNA was significantly decreased compared to those in control-siRNA-transfected cells (Figure ...production and cell ... See full document
13
Evaluating methods for isolating total RNA and predicting the success of sequencing phylogenetically diverse plant transcriptomes.
... study and the collective experience of the many collaborators involved in 1KP, we are able to provide plant researchers with practical recommendations for maximizing success of isolating high-quality total ... See full document
12
SMP de Oliveira , AF Barbosa, ML Vilela, EF Rangel, RP Brazil,
... For synthesis of first strand cDNA molecules 100 ng of total RNA isolated from sugar and blood fed midguts of ...Liang and Pardee ...midgut RNA was mixed with ...purified ... See full document
7
A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography
... of RNA in many biological functions has been recognized, broadening the scope of RNA research activities, from basic to applied sciences, also aiming the transla- tion to clinical ...preparation and ... See full document
10
SARS-coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum.
... Positive-strand RNA viruses, a large group including human pathogens such as SARS-coronavirus (SARS-CoV), replicate in the cytoplasm of infected host ...viral RNA synthesis range from distinct ... See full document
18
The mini-chromosome maintenance (Mcm) complexes interact with DNA polymerase α-primase and stimulate its ability to synthesize RNA primers.
... intrinsic RNA primer synthesis activity were always observed (our unpublished ...Mcm and primase is weak due to the transient nature of their ...GINS and Cdc45 interact with both polymerase ... See full document
14
J. Braz. Chem. Soc. vol.26 número5
... the isolation of these ...sources, and its intriguing biological activity, more than 100 synthetic routes have been developed since the epibatidine structure was ...formal and total syntheses ... See full document
14
Quím. Nova vol.33 número10
... 5 and 6 increased to 36% with amide 6 as the major product (Table 1, entry ...3 and 4) and the use of the sodium enolate of BSA provided neither amide 5 nor 6 (Table 1, entry ...5 and 6 (56%) ... See full document
4
Ticks and Tick-borne Diseases
... efficiencies, and optimal reagent ...detect and quantify the transcripts involved in the synthesis of different AChEs and ESTs of ...ribosomal RNA gene of ... See full document
8
Thermostable DNA polymerase from a viral metagenome is a potent RT-PCR enzyme.
... where RNA target copies may approach single molecule ...thermostable and background reduction during reverse transcrip- tion using hot start methods for these RTs are not ...MS2 and the influenza ... See full document
13
Artroplastia Total da Anca – Normas de Orientação Clínica
... artroplastia total da anca (ATA) deve ser considerada em pessoas com artropatia da anca que mantenham dor ou limitação na realização das atividades de vida diárias, após tentativa de tratamento conservador ou de ... See full document
10
Isolation of dihydroflavonol 4-reductase cDNA clones from Angelonia x angustifolia and heterologous expression as GST fusion protein in Escherichia coli.
... white/blue and white flower ...synthase and flavanone 3-hydroxylase activity remained ...cloning and characterization of a DFR of Angelonia flowers was carried out for the first ...DFR cDNA ... See full document
9
temas relacionados