[PDF] Top 20 IN VITRO CHARACTERIZATION OF GE NEGATIVE BOVINE HERPESVIRUS TYPES 1.1 (BHV-1.1) AND 1.2A (BHV-1.2A)
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IN VITRO CHARACTERIZATION OF GE NEGATIVE BOVINE HERPESVIRUS TYPES 1.1 (BHV-1.1) AND 1.2A (BHV-1.2A)
... p.f.u. of the appropriate virus to adsorb on either MDBK or Ebtr monolayers for 1 hour at ...days. In a control experiment, additional cell monolayers were infected with 500 ...p.f.u. of all ... See full document
5
CONSTRUCTION AND CHARACTERIZATION OF A BOVINE HERPESVIRUS 5 MUTANT WITH A DELETION OF THE GI, GE AND US9 GENES
... Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South ...framework of developing a differential vaccine against BoHV-5, a deletion mutant ... See full document
7
Production and characterization of monoclonal antibodies to a Brazilian bovine herpesvirus type 5
... antigens in MDBK cells infected with a glycoprotein C-deleted BHV- 1 mutant (IBRVdlTKdlgIII) (23) by ...ability of individual mAbs to react with BHV- 5 antigens in tissues after routine ... See full document
9
In vitro interaction of bovine herpesvirus 1 with uterine tube epithelial cells and oocytes
... pmol of each sense and anti- sense primers (5’ CCT CTG TGA ACT GCA TCG TGG A 3’; 5’ TAG CCC TCG ATC TGC TGG AGG C ...region of BoHV-1 gB gene (Vilcek et ...for 1 minute, synthesis at 72°C for ... See full document
6
A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain
... products, of which 10 are envelope ...roles in viral biology, pathogenesis and con- stitute major targets for the host immune system ...replication in cell culture and in vivo and, as such, ... See full document
9
Field evaluation of safety during gestation and horizontal spread of a recombinant differential bovine herpesvirus 1 (BoHV-1) vaccine
... the gE- vaccine was capable of inducing high levels of neutralizing antibodies on vaccinated ...transfer of antibodies to the newborn, as shown for other herpesviruses (Casal et ...also ... See full document
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Evaluation of developmental changes in bovine in vitro produced embryos following exposure to bovine Herpesvirus type 5
... washed in TALP medium (Tyrode medium with added bicarbonate buffer and supplemen- ted with 6 mg BSA per milliliter) and centrifuged at 200 X g for 5 ...diluted in IVF medium (TALP medium supplemented with 3 ... See full document
10
Safety and immunogenicity of a glycoprotein E gene-deleted bovine herpesvirus 1 strain as a candidate vaccine strain
... out of a Brazil- ian BoHV-1 SV56/90 strain (Weiblen et ...procedures of construction and in vitro characterization of the recombinant were published recently (Weiss et ... See full document
8
Experimental infection of rabbits with a recombinant bovine herpesvirus type 5 (BoHV-5) gI, gE and US9-negative
... detected in sera from animals infected with both the wild type and recombinant BoHV-5 (Figure ...levels of neutralizing antibodies detected until day 60 pi, when the Dx treatment took place, were similar ... See full document
6
Efficacy of a gE-deleted, bovine herpesvirus 1 (BoHV-1) inactivated vaccine
... Bovine herpesvirus 1 (BoHV-1), a member of the Alphaher- pesvirinae subfamily, commonly known as infectious bovine rhinotracheits (IBR) virus, is an important cause of ... See full document
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Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
... performances of viruses within a same subtype ...(66.1%) of the total 281 positive sera detected by the two viruses added together (Table ...sensitivity of 85.9% over the total number of ... See full document
8
Prokaryotic expression of a truncated form of bovine herpesvirus 1 glycoprotein E (gE) and its use in an ELISA for gE antibodies
... expression of a truncated form of bovine herpesvirus 1 (BoHV- 1) glycoprotein E (gE) for use as immunodiagnostic ...acids) of BoHV-1 gE - that shares ... See full document
6
Detection of antibodies against bovine respiratory syncytial virus (BRSV) in dairy cattle with different prevalences of bovine herpesvirus type 1 (BoHV-1) in São Paulo State, Brazil
... role in the development of respiratory disease in cattle herds, especially in dairy farms (KIMMAN et ...BRSV in different countries often reveal prevalences higher than 40% (DURHAM; ... See full document
6
Etiologic diagnosis of bovine infectious abortion by PCR
... one of the organisms included in the panel, but with concentrations of genomic DNA in the sample that was below the limit of detection of the ...potential of PCR as an ... See full document
8
Dexamethasone-induced reactivation of bovine herpesvirus type 5 latent infection in experimentally infected rabbits results in a broader distribution of latent viral DNA in the brain
... DNA in some areas of the brain of experimentally infected calves ...latency in several ar- eas of the brain of experimentally infected calves and that the distribution of ... See full document
9
In vitro maintenance of IDE8 cells using two types of bovine serum.
... Nos primeiros 15 dias de cultivo, os dois grupos de culturas apresentaram pequena queda na percentagem de células viáveis, possivelmente pela adaptação inicial em placas, pois antes de se iniciar o experimento a cultura ... See full document
4
Epidemiologia, sinais clínicos e distribuição das lesões encefálicas em bovinos afetados por meningoencefalite por herpesvírus bovino-5.
... necropsia e histopatológicos característicos e a confirmação pela detecção viral em cultivo celular, imunofluorescência ou PCR. Em 3 surtos, perfazendo 14 bovinos afetados, a confirmação do diag- nóstico foi realizada ... See full document
10
MATERIALS AND METHODS Virus and Cells
... disturbances in dairy and beef cattle can be responsible for significant economic losses due to high death ...involved in central nervous system (CNS) diseases in cattle ...range of very ... See full document
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Caracterização genotípica de linhagens de Prototheca zopfii isoladas do leite de vacas e sensibilidade in vitroa antimicrobianos e anti-sépticos
... “in vitro” da gentamicina, enquanto Melville (2000) encontrou 100,0% de nennibilidade parcial em linhagenn de inoladan de mantite, frente ao menmo ...“in vitro” da gentamicina (66,7%) frente ... See full document
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Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil
... diagnosis of the four events was based on the clinical signs, high mortality and pathological findin- ...typical of neonatal CHV-1 infection, characterized by a multisystemic necrohemorrhagic disease ... See full document
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