Tomados em conjunto, os resultados indicam que:
• A CQ promove uma alteração fenotípica e funcional das DCs capaz de interferir na função das células T CD4+, devido à diminuição na expressão dos marcadores de superfície das DCs, à alteração na expressão genica das citocinas inflamatórias e à capacidade de modular a atividade das células T para um perfil de resposta tolerogênica;
• Além disso, os dados obtidos sugerem que o efeito indutor de tolDCs pela CQ depende, em parte, de uma inibição da ativação da via não canônica do NF-B. Isso porque observamos os mesmos efeitos sobre as DCs obtidas de camundongos tratadas com a CQ nas DCs que foram tratadas in vitro com a CQ na presença do ativador da via (LPS);
• Por fim, os resultados mostram que a presença de um ambiente imunologicamente ativado pela EAE não foi suficiente para reverter o efeito terapêutico das DCs tratadas com a CQ sugerindo uma provável relação entre o efeito terapêutico dessas tolDCs e a inibição a via não canônica do NF-B promovida pela CQ.
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