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PP2A1 binding, cell transducing and apoptotic properties of Vpr(77-92): a new functional domain of HIV-1 Vpr proteins.

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Academic year: 2017

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Figure 1. Analysis of HIV-1 Vpr and PP2A1 interaction. (A) 16 mg of PP2A1 holoenzyme purified from pig brain was loaded on a 1 mL HIV-1 strain 89.6-Vpr-T-agarose column equilibrated in buffer C (25 mM TrisHCl pH 7.4, 50 mM NaCl, 0.1 mM EDTA)
Table 2. Origin of peptides containing wild type and mutated Vpr sequences derived from 89.6 and pNL4.3 sequences used in this study.
Figure 4. To monitor apoptosis in Jurkat cells treated with DPT-Vpr peptides, we used Annexin V (upper panel) or DiOC6 (lower panel) assays

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