Urinary Glycosaminoglycans Excretion and the Effect of Dimethyl
Sulfoxide in an Experimental Model of Non-Bacterial Cystitis
Roberto Soler, Homero Bruschini, Jose C. Truzzi, Joao R. Martins, Niels O. Camara, Maria T. Alves, Katia R. Leite, Helena B. Nader, Miguel Srougi, Valdemar Ortiz
Division of Urology (RS, JCT, VO), Division of Molecular Biology (JRM, HBN), Division of Nephrology (NOC) and Division of Pathology (MTA), Federal University of Sao Paulo, UNIFESP, and Division of Urology (HB, KRL, MS), University of Sao Paulo, USP, Sao Paulo, SP, Brazil
ABSTRACT
Purpose: :HUHSURGXFHGDQRQEDFWHULDOH[SHULPHQWDOPRGHOWRDVVHVVEODGGHULQÀDPPDWLRQDQGXULQDU\JO\FRVDPLQRJO\-FDQV*$*H[FUHWLRQDQGH[DPLQHGWKHHIIHFWRIGLPHWK\OVXOIR[LGH'062
Materials and Methods:)HPDOHUDWVZHUHLQVWLOOHGZLWKHLWKHUSURWDPLQHVXOIDWH36JURXSVRUVWHULOHVDOLQHFRQWURO JURXSV$WGLIIHUHQWGD\VDIWHUWKHSURFHGXUHKXULQHDQGEODGGHUVDPSOHVZHUHREWDLQHG8ULQDU\OHYHOVRIK\DOXURQLF DFLG+$DQGVXOIDWHGJO\FRVDPLQRJO\FDQV6*$*ZHUHGHWHUPLQHG$OVRWRHYDOXDWHWKHHIIHFWRI'062DQLPDOV ZHUHLQVWLOOHGZLWKHLWKHU'062RUVDOLQHKRXUVDIWHU36LQVWLOODWLRQ7RHYDOXDWHWKHHIIHFWRI'062LQKHDOWK\ EODGGHUVUDWVZHUHLQVWLOOHGZLWK'062DQGFRQWUROVZLWKVDOLQH
Results: ,QWKH36JURXSVEODGGHULQÀDPPDWLRQZDVREVHUYHGZLWKSRO\PRUSKRQXFOHDUFHOOVGXULQJWKH¿UVWGD\VDQGO\P-SKRPRQRQXFOHDULQWKHODVWGD\V+$DQG6*$*KDGSHDNVRIXULQDU\H[FUHWLRQDWWKHst and 7thGD\DIWHU36LQMHFWLRQ
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ZHUHVLPLODULQDOOJURXSV
Conclusions:,QFUHDVHGXULQDU\OHYHOVRI*$*ZHUHDVVRFLDWHGZLWKEODGGHULQÀDPPDWLRQLQD36LQGXFHGF\VWLWLVPRGHO '062VLJQL¿FDQWO\UHGXFHGWKHLQÀDPPDWRU\SURFHVVDIWHUXURWKHOLDOLQMXU\&RQYHUVHO\WKLVGUXJSURYRNHGPLOGLQÀDP -PDWLRQLQQRUPDOPXFRVD'062WUHDWPHQWZDVVKRZQWRLQÀXHQFHXULQDU\+$H[FUHWLRQ
Key words: cystitis; dimethyl sulfoxide; glycosaminoglycans; rats; protamines
Int Braz J Urol. 2008; 34: 503-11
INTRODUCTION
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Impaired barrier function of bladder
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MATERIALS AND METHODS
Animals and Induction of Bladder
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catheter was inserted into the bladder and the urine
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Experimental Groups
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Histopathology
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Measurement of Urinary Hyaluronic Acid
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Measurement of Urinary Sulfated GAG
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Statistical Analysis
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RESULTS
Histopathology
PS and Control Groups
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DMSO Groups
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GAG Measurement
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COMMENTS
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Table 1 – Pathological features in DMSO groups.
Group EdemaJUDGH Vascular Congestion
JUDGH PMN Count (median)
Mast Cell Count(median)
LMN Count
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also described in biopsies and cell culture obtained
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Table 2 – Urinary GAG levels in DMSO groups.
Group HA/Cr QJPJ S-GAGJPJ
1 day
Q 7 daysQ Q 1 day 7 daysQ
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CONFLICT OF INTEREST
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EDITORIAL COMMENT
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Dr. Robert E. Hurst
Oklahoma University Cancer Institute and Oklahoma University Health Sciences Center Oklahoma City, Oklahoma, USA
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Accepted after revision:
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Correspondence address:
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