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Fluorescent labeling of antibody fragments using split GFP.

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Figure 1. Split-GFP labeling of scFvs. A. GFP complementation vectors. The scFv-D1.3-GFP11 construct was cloned into a pEP based vector (left) or a yeast display vector (right)
Figure 2. Comparison of antigen binding activity assessed by ELISA. The antigen binding activity was analyzed by ELISA using different concentrations of scFv-D1.3 and scFv-D1.3-GFP11 scFvs (ranging from 0.6 to 300 nM)
Figure 3. Progress curves analysis of the complemented fluorescence. A. Progress curves of complemented fluorescence using purified protein
Figure 5. FLISA results at different complemented scFv-GFP11 concentrations. scFv-D1.3-GFP11 was used in a FLISA assay with binding between the scFv and its specific antigen assessed by complemented fluorescence
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