Aflp analysis
Metabolic profiles and cDNA-AFLP analysis of Salvia miltiorrhiza and Salvia castanea Diel f. tomentosa Stib.
... cDNA-AFLP analysis of four samples were employed to identify novel genes potentially involved in phenolic compounds and tanshinones biosynthesis, including the red roots from the two species and two ...
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AFLP analysis of genetic diversity in determinate and indeterminate snap bean accessions
... the AFLP analysis, and the combinations E-AAG/M-CTC, E-ACT/M-CTT and E-ACC/M-CTT were ...selective AFLP combinations revealed 485 informative loci in total, and the combination E-ACC/M-CTT detected ...
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cDNA-AFLP analysis of gene expression differences between the flower bud and sprout-shoot apical meristem of Angelica sinensis (Oliv.) Diels
... These 26 TDF sof high expression level were excised from the polyacrylamide gels and re-amplified with the original primer sets used for cDNA-AFLP analysis. After the isolated TDFs were cloned, five ...
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Genetic divergence among tomato leafminer populations based on AFLP analysis
... Resumo – O objetivo deste trabalho foi determinar a divergência genética entre oito populações de Tuta abso- luta (Meyrick) (Lepidoptera: Gelechiidae), provenientes dos Estados do Espírito Santo (Santa Tereza), Goiás ...
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Genetic diversity of Hirsutella thompsonii isolates from Thailand based on AFLP analysis and partialb -tubulin gene sequences
... Our research showed that, in general, H. thompsonii isolates obtained from various mite species showed high polymorphism for the molecular markers studied. Am- plified fragment length polymorphism (AFLP) has ...
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AFLP marker analysis revealing genetic structure of the tree Parapiptadenia rigida (Benth.) Brenan (Leguminosae-Mimosoideae) in the southern Brazilian Tropical Rainforest
... Genomic DNA was isolated from approximately 0.5 g of fresh leaves using the CTAB method, as described by Doyle and Doyle (1987). The DNA concentration was estimated using a fluorometer (DyNA Quant 200, Höfer- Pharmacia), ...
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Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius
... for analysis. RAPD and AFLP analyses were done on pooled samples from 20 plantlets of B. setifolius var. pictus (Bp1), B. setifolius var. brevifolius (Bb1) and B. setifolius var. setifolius (Bs1). Five ...
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Análise da estrutura genética da população de Hemileia vastatrix com base no marcador AFLP
... Os marcadores foram obtidos utilizando o Kit AFLP ® Analysis System I (Invitrogen) de acordo com manual de instruções, com adaptações. Para isso, 250 ng/ μl de DNA foram digeridos com as endonucleases de ...
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Diversidade molecular entre populações de Spodoptera frugiperda no Brasil avaliada por marcadores AFLP.
... Para a extração de DNA, foi utilizado o protocolo fornecido pelo kit Dneasy Blood & Tissue (Qiagen, Valencia, CA, EUA). A quantificação do DNA das amostras foi feita em espectrofotômetro NanoVue Plus (GE Healthcare ...
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CARACTERIZAÇÃO MOLECULAR DE GENÓTIPOS DE MAMOEIRO COM USO DE MARCADORES DO TIPO AFLP
... Several authors (VAN DROOGENBROECK et al., 2002; OCAMPO PÉREZ et al., 2007; OLIVEIRA et al., 2010b) reported richness and high diversity of C. papaya resources in several regions, mainly analyzing natural populations and ...
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Genetic relationships among Arachis species based on AFLP
... an analysis of 14 rice accessions, ...other AFLP analysis of variation within 6 divergent geno- types from three botanical varieties of ...loci analysis of ...
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Análise da diversidade genética por AFLP e identificação de marcadores associados à resistência a doenças em videira.
... ABSTRACT - The grapevine cultivars A 1976, CG 87746, CNPUV 154-27, Crimson Seedless, Gota de Ouro, Itália, Seyve Villard 12327 and Seyve Villard 12375 were analyzed to establish the genetic relationship and to identify ...
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Genetic diversity revealed by AFLP markers in Albanian goat breeds
... AFLP analysis using three primer combinations re- vealed 107 polymorphic markers. here was no sig- niicance between Nei’s expected heterozygosity and the Shannon index, but the rank correlation of these ...
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Marcadores AFLP diferenciam Colletotrichum gossypii de C. gossypii var. cephalosporioides
... for AFLP analysis was obtained from seven-day-old fungal mycelia grown in liquid medium, using the Dneasy Qiagen ...the AFLP technique 318 polymorphic bands were selected to estimate similarities ...
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Similaridade genética entre cultivares de marmeleiro avaliadas por marcadores AFLP.
... Para a extração de DNA, foi utilizado o método CTAB (brometo de cetiltrimetilamônio) proposto por Doyle & Doyle (1990), seguido da análise de AFLP de acordo com o protocolo do fabricante Kit AFLP ...
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Caracterização de Streptococcus suis por sorotipagem, SE-AFLP e perfil de virulência
... Streptococcus suis is one of most important pathogens in the swine industry worldwide. Despite its importance, studies of S. suis characterization in South America are still rare. This study evaluates S. suis isolates ...
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Analysis of genetic diversity in Portuguese Ceratonia siliqua L. cultivars using RAPD and AFLP markers
... However, AFLP analysis in this study was a more reliable and robust genetic molecular-marker assay with higher bootstrap values for each ...the AFLP analysis and five main groups in RAPD ...
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Diversidade Genética entre Biótipos de Proso Millet (Panicum miliaceum) Revelada pela Técnica de AFLP
... through analysis performed by RAPD (Colosi & Schaal, 1997) showed that Minnesota-Cambridge biotype was genetically intermediate between wild and domesticated proso ...of AFLP polymorphic bands detected ...
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Taxa de cruzamento entre manga 'Haden' e 'Tommy Atkins' estimada com marcadores microssatélites e AFLP
... of AFLP or microsatellite markers and by the mLTR ...the analysis based on seven AFLP markers, and from ...direct AFLP and microsatellite allele counting, which indicates that the multilocus ...
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Genetic similarity of Jatropha curcas accessions based on AFLP markers
... RESUMO - Foram determinadas as relações genéticas entre acessos de pinhão-manso (Jatropha curcas), com base no marcador AFLP. Um conjunto de 50 indivíduos de 12 acessos de pinhão-manso foi analisado com dados ...
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