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Aqueous two-phase systems

Aqueous two-phase systems: fundamentals and applications for partitioning/purification of proteins.

Aqueous two-phase systems: fundamentals and applications for partitioning/purification of proteins.

... AQUEOUS TWO-PHASE SYSTEMS: FUNDAMENTALS AND APPLICATIONS FOR PARTITIONING/PURIFICATION OF ...of aqueous two- phase systems (ATPS) to extraction processes has been ...

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Recovery of pectinases from Aspergillus niger using aqueous two-phase systems / Recuperação de pectinases de Aspergillus niger usando sistemas aquosos bifásicos

Recovery of pectinases from Aspergillus niger using aqueous two-phase systems / Recuperação de pectinases de Aspergillus niger usando sistemas aquosos bifásicos

... by aqueous two-phase systems ...ATPS systems tested consisted in the combinations of polyethylene glycol (PEG) and potassium phosphate and sodium citrate, alcohol (ethanol, n-propanol ...

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LIQUID-LIQUID EQUILIBRIUM DATA, VISCOSITIES, DENSITIES, CONDUCTIVITIES, AND REFRACTIVE INDEXES OF POLY (VINYL PYRROLIDONE)+ TRI-SODIUM CITRATE + UREA AQUEOUS TWO-PHASE SYSTEMS AT DIFFERENT PH

LIQUID-LIQUID EQUILIBRIUM DATA, VISCOSITIES, DENSITIES, CONDUCTIVITIES, AND REFRACTIVE INDEXES OF POLY (VINYL PYRROLIDONE)+ TRI-SODIUM CITRATE + UREA AQUEOUS TWO-PHASE SYSTEMS AT DIFFERENT PH

... mass) aqueous two-phase systems at 25°C and pH values of ...water two-phase systems were measured versus pH ...the aqueous two-phase systems ...

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An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems

An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems

... polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic ...step. Two single bands in SDS-polyacrylamide gel electrophoresis and increased ...

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STUDY OF ALPHA-AMYLASE OBTAINED BY SOLID STATE FERMENTATION OF CASSAVA RESIDUE IN AQUEOUS TWO-PHASE SYSTEMS

STUDY OF ALPHA-AMYLASE OBTAINED BY SOLID STATE FERMENTATION OF CASSAVA RESIDUE IN AQUEOUS TWO-PHASE SYSTEMS

... in Aqueous Two-Phase Systems (ATPS) was determined using systems consisting of polyethylene glycol (PEG) and potassium phosphate ...for two independent variables: fermentation ...

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Purification of C-phycocyanin from Spirulina platensis in Aqueous Two-Phase Systems using an Experimental Design

Purification of C-phycocyanin from Spirulina platensis in Aqueous Two-Phase Systems using an Experimental Design

... C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a ...

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Partition of Glucose Oxidase from Aspergillus niger in Aqueous Two-Phase Systems Based on Salt and Polyethylene Glycol

Partition of Glucose Oxidase from Aspergillus niger in Aqueous Two-Phase Systems Based on Salt and Polyethylene Glycol

... in aqueous-two phase systems will also lead to the ability to predict the partitioning of specific materials (Silva and Franco, ...salt phase which promotes mass transfer, and as a ...

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Partition study of textile dye Remazol Yellow Gold RNL in aqueous two-phase systems

Partition study of textile dye Remazol Yellow Gold RNL in aqueous two-phase systems

... from aqueous systems are either inefficient or too expensive to be adopted by smaller textile ...on aqueous two-phase system (ATPS) using salts and either polymers or ionic liquids to ...

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Braz. J. Chem. Eng.  vol.20 número4

Braz. J. Chem. Eng. vol.20 número4

... using aqueous two-phase systems for extracting different proteins and ...and phase separation during operation of the column was ...

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Braz. J. Chem. Eng.  vol.22 número3

Braz. J. Chem. Eng. vol.22 número3

... models. Phase diagrams have been reported for a large number of polymer-polymer systems (Albertsson, 1986; Zaslavsky, 1995); however, experimental liquid-liquid equilibrium (LLE) data for aqueous ...

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Efeito hidrofóbico de macromoléculas sobre a partição de cianocomplexos em sistemas aquosos bifásicos

Efeito hidrofóbico de macromoléculas sobre a partição de cianocomplexos em sistemas aquosos bifásicos

... The phase diagrams determined for the F68/ salt/ water and L35/ salt/ water systems at 25°C are shown in ...The systems separated into two isotropic phases, the top and bottom phases being ...

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INFLUENCE OF pH ON THE PARTITION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND HEXOKINASE IN AQUEOUS TWO-PHASE SYSTEM

INFLUENCE OF pH ON THE PARTITION OF GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND HEXOKINASE IN AQUEOUS TWO-PHASE SYSTEM

... of aqueous solutions of two polymers, such as PEG and dextran, or a polymer and an electrolyte solution, such as PEG and phosphate salt, is a method based on the partition of proteins between two ...

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Braz. J. Chem. Eng.  vol.27 número2

Braz. J. Chem. Eng. vol.27 número2

... for aqueous two- phase systems for consistency ...liquid phase-separation that is not consistent with the physics underlying the ...term, aqueous two-phase ...

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Braz. J. Chem. Eng.  vol.19 número2

Braz. J. Chem. Eng. vol.19 número2

... carbonate aqueous two- phase systems were used to estimate the molecular interaction and nonrandomness parameters of the NRTL model for the activity ...

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Braz. J. Chem. Eng.  vol.22 número3

Braz. J. Chem. Eng. vol.22 número3

... to aqueous two-phase systems (Jafarabad et ...with aqueous biphasic systems (Porto et ...dispersed phase velocity, system composition and discs rotation speed on ...

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Braz. J. Chem. Eng.  vol.21 número3

Braz. J. Chem. Eng. vol.21 número3

... Abstract - Mixtures containing compounds that undergo hydrogen bonding show large deviations from ideal behavior. These deviations can be accounted for through chemical theory, according to which the formation of a ...

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The Effect of pH on Bromelain Partition from Ananas

The Effect of pH on Bromelain Partition from Ananas

... The bromelian, according to Sigma (2006), had a price about U$ 1,075.40 for kilogram of purified material, at 30% of protein contents. Thus, the recovery these enzymes from pineapple steam will add value this ...

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Recovery of Ascorbic Oxidoreductase from Crude Extract with an Aqueous Two-phase System in a Perforated Rotating Disc Contactor

Recovery of Ascorbic Oxidoreductase from Crude Extract with an Aqueous Two-phase System in a Perforated Rotating Disc Contactor

... Investigations with York-Sheibel columns (Raghav Rao et al., 1991) using ATPS of PEG 4000 and potassium phosphate salts and sodium sulfate salts showed mass transfer coefficients three times higher than the ones obtained ...

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Braz. J. Chem. Eng.  vol.21 número4

Braz. J. Chem. Eng. vol.21 número4

... conclusion, two-phase aqueous micellar systems can be considered a promising new alternative for the purification of G6PD, as well as of other hydrophilic ...The two-phase ...

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Braz. J. Chem. Eng.  vol.31 número4

Braz. J. Chem. Eng. vol.31 número4

... these systems, an aqueous surfactant solution, under the appropriate solution conditions, spontane- ously separates into two predominantly aqueous, yet immiscible, liquid phases, one of which ...

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