biochemical characterization
Biochemical characterization of the GM2 gangliosidosis B1 variant
... mutation. Biochemical characterization of this lysosomal disease is carried out using negatively charged synthetic α subunit-specific sulfated substrates, since Hex A isoenzyme heat-inactivation assays are ...
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Physiological and biochemical characterization of mango tree with paclobutrazol application via irrigation
... followed by changes in the hormonal balance and carbohydrates production. This study aimed at evaluating the physiological and biochemical variables of mango tree (Palmer cultivar) by applying paclobutrazol doses ...
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Biochemical characterization of interactions between kinetochore protein complexes
... KEY WORDS: RZZ complex; Spindly; Rod; ZW10; Zwilch; SAC; Dynein; Kinetochores; Microtubules; Mitosis; Centromere; Cell Cycle; Yeast two Hybrid; Binding Assays with [r] ...
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PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF IgG FROM SERUM OF YAK ( BOS GRUNNIENS )
... Boden V, Winzerhing JJ, Vijayalakshmi M and Porath J.(1995). Rapid one step purification of goat immunoglobulin by immobilized metal ion affinity chromatography. J. Imm. Methods. 181: 225-232. Kakker NK and Goel MC. ...
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Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros
... Thereafter, BPPs were isolated from many snake venoms and shown to potentiate contraction in the iso- lated guinea pig ileum and to increase the hypotensive effect of BK, by inhibiting BK degradation [20]. The ...
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Biochemical characterization of a phospholipase A
... After rechromatography of the fractions of in- terest, a protein with a molecular mass of 13,896.47 Da, devoid of enzymatic activity and with high identity with snake venom Lys49 PLA 2 h[r] ...
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Extraction and Biochemical Characterization of Sulphated Glycosaminoglycans from Chicken Keel Cartilage
... Qualitative analysis: GAGs in extracted semi-purified polysaccharides samples (n=3) was identified by FTIR spectroscopy technique using potassium bromide pellet and by comparing with[r] ...
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Biochemical Characterization of KhtTU, a K+/H+ Antiporter from Bacillus subtilis
... His-tagged KhtT was used as bait protein in a pull-down assay performed while testing for KhtS expression using the pRSFDuet:khtT+khtS construct.. The pull-down assay was done [r] ...
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Morphological and biochemical characterization of the aetiological agents of white piedra
... logical, biochemical and molecular characterisations, identified six human pathogenic species: Trichosporon ovoides Behrend, Trichosporon inkin (Oho ex Ota) do Carmo-Souza and van Uden, Trichosporon asteroides, ...
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Ixodes ricinus tick lipocalins: identification, cloning, phylogenetic analysis and biochemical characterization.
... Conclusions/Significance: This work confirms that, regarding their biochemical properties, expression profile, and sequence signature, lipocalins in Ixodes hard tick genus, and more spec[r] ...
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Genetic and biochemical characterization of hemicellulose polysaccharides utilization in Bacillus subtilis
... The biochemical and structural characterization of the native ABN (Abn2) from ...the biochemical and structural characterization of a second mutant of H318 (H318Q) was ...
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PARTIAL PURIFICATION AND IMMUNE-BIOCHEMICAL CHARACTERIZATION OF DOG SERUM IMMUNOGLOBULIN G
... Western blot analysis of bovine IgG and IgM purified from bovine blood serum as well as whole immunoglobulin fractions of bovine and ovine serum with IVA-285 showed a molecular weight in[r] ...
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Biochemical characterization of enzyme fidelity of influenza A virus RNA polymerase complex.
... Unlike the UTP stop sites, it is difficult to observe the first stop sites induced by the deletion of other three NTPs (A, G and C) because the first 5 nucleotides in the 39 end conserved sequence of the IAV genomes ...
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Biochemical characterization of three BLT receptors in zebrafish.
... The hBLT1 and zebra- fish Blt1 were expressed on the cell surface at similar levels ( Fig. 4A ), and hBLT2, zebrafish Blt2a, and Blt2b were expressed on the cell surface at similar level[r] ...
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Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome.
... doi:10.1371/journal.pone.0159345.g005.. Structural model of MZ0003 and mutagenesis. A) Overall structure of MZ0003: α-helix of regions that aligned with the M. thermophilia StGE2 sequenc[r] ...
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Biochemical characterization of uracil phosphoribosyltransferase from Mycobacterium tuberculosis.
... the biochemical studies on MtUPRT mode of action here described provide a solid support on which to base future efforts on gene replacement towards the development of efficient prophylactic strategies to combat ...
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EVALUATION AND BIOCHEMICAL CHARACTERIZATION OF A DISTINCTIVE PYOVERDIN FROM A PSEUDOMONAS ISOLATED FROM CHICKPEA RHIZOSPHERE
... Quantitative estimation showed that the organism produced a maximum of about 1000 μ g mL -1 of hydroxamate type of siderophore in culture medium after 24 hours of incubation which re[r] ...
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Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi
... In Trypanosomatidae, different studies have report- ed the presence of SOD activities. T. brucei (Dufernez et al. 2006, Wilkinson et al. 2006), Leishmania spp. (Bogdan et al. 1990, Genetu et al. 2006), and Phytomo- nas ...
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Biochemical characterization of novel retroviral integrase proteins.
... We were able to express and extract alpharetrovirus LPDV IN, betaretrovirus JSRV IN and MMTV IN, and gammaretrovirus Rev-A IN. However, despite extensive effort (Fig. 1B), we were unable to purify accountable amounts of ...
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Biochemical and molecular characterization of barley plastidial ADP-glucose transporter (HvBT1).
... In cereals, ADP-glucose transporter protein plays an important role in starch biosynthesis. It acts as a main gate for the transport of ADP-glucose, the main precursor for starch biosynthesis during grain filling, from ...
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