cell free system
Replication independent formation of extrachromosomal circular DNA in mammalian cell-free system.
... To promote the investigation of eccDNA phenomenon in mammalian cells we established a cell-free system for eccDNA formation. We used mouse DNA as a template through this study since mouse genome ...
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Escherichia coli NemA is an efficient chromate reductase that can be biologically immobilized to provide a cell free system for remediation of hexavalent chromium.
... enzyme-based system for Cr(VI) remediation would not suffer these limitations, but would face additional challenges from the extracellular environment; potential stability issues, a lack of reducing equivalents ...
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Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes.
... (cell free) expression has emerged in recent times as a promising alternative option in order to synthesize large quantities of membrane proteins, and the focus of this report was to evaluate the potential ...
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Matriptase autoactivation is tightly regulated by the cellular chemical environments.
... We next sought to investigate whether spontaneous and/or acid-accelerated matriptase activation observed in vitro occurs in intact cells. To this end, 184 A1N4 mammary epithelial cells were exposed to 150 mM phosphate ...
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Antioxidant effects of quercetin and naringenin are associated with impaired neutrophil microbicidal activity
... and cell- free ...first cell-free system, indicated that significant inhibition of MPO chlorinating activity can be induced by this ...(cell-free system) to 50% ...
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Alpha 1-antitrypsin does not inhibit human monocyte caspase-1.
... Neither cell free active caspase-1, live cell models of caspase-1 function, nor fresh human whole blood cell models of IL-1β release were inhibited by exogenous ...
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A TNM Staging System for Nasal NK/T-Cell Lymphoma.
... months, with 1- and 3-year OS rates of 15% and 4%, respectively. The survival time of the unique long-term survivor lasted for 36.9 months by the time of the last follow-up, while this patient had two lesions in the ...
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Avaliação do efeito da liraglutida, um análogo do GLP-1, na proliferação das células pré-osteoblásticas MC3T3 E1
... the cell cycle or initiates the apoptotic ...the cell cycle, apoptosis, autophagy, senescence, DNA metabolism, angiogenesis, cellular differentiation and the immune response (Kaminskyy and Zhivotovsky, ...
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Constraints on free parameters of the simplest bilepton gauge model from the neutral kaon system mass difference
... In this Brief Report we have studied the implications of the Dm mass difference on free parameters of the 3-3-1 model. However, differently from previous calculations @13,15#, here we are taking into account the ...
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J. Appl. Oral Sci. vol.22 número3
... the distribution Normal, where only 0.5% of the sample values of r will deviate from zero to within ±0.50. This is justiied by the sample size resulting statistically significant. The sample size of 23 subjects (10 male ...
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Changes in the pool of free fatty acids in ovine, bovine and caprine milk fats, effected by viable cells and cell-free extracts of Lactococcus lactis and Debaryomyces vanrijiae
... lipolytic system – which might, in addition, have a particularly high affinity to ovine milk fat; conversely, an analytical artifact could as well have arisen, hardly with statistical ...
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Farm2.0: Using Wordpress to Manage Geocontent and Promote Regional Food Products
... Farm2.0 system is product promotion, and appealing graphic design is regarded as an essential aspect of branding and sales strategies (Schmitt, 1997; Gobé, 2001; Holland, 2001; Crilly, ...
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Neutrophilic cell-free exudate induces antinociception mediate by the protein S100A9
... neutrophilic cell-free exudates obtained of mice injected with glycogen was ...of cell migration of the peritoneal exudate or the nociceptive response of animals was ...Peritoneal ...
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Cell free layer (CFL) measurements in complex geometries contractions and bifurcations
... maio purpose is ta examine the effect af different artificial cantractians, bifurca- tians and canftuences, different hematocrits (Hct) and different velocities in th[r] ...
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miR-155 Controls Lymphoproliferation in LAT Mutant Mice by Restraining T-Cell Apoptosis via SHIP-1/mTOR and PAK1/FOXO3/BIM Pathways.
... In addition to this effect of PAK1 on JNK activation, we previously described a role for a PAK1/PLCγ-1/Bam32 complex in ERK activation in both normal CD4 + T cells [7] and in CD4 + T cells isolated from LAT-KI mice [5]. ...
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Adaptive Free Space Management of Online Placement for Reconfigurable Systems
... adaptive free space management for finding candidate space with rectangular or nonrectangular to place newly arriving ...adaptive free space management uses two procedures to find all feasible candidate ...
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The effect of carbon and nitrogen sources on bovicin HC5 production by Streptococcus bovis HC5
... Because cell mass pro- duction and bacteriocin activity are not readily related, the effect of nitrogen sources on bovicin HC5 production appears to involve multiple physiological ...
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Microbial spy games and host response: roles of a Pseudomonas aeruginosa small molecule in communication with other species.
... immune system, (opportunistic) microbial pathogens have developed sophisticated cell–cell communication systems termed quorum sensing (QS) that allow exchange of critical ...immune system, ...
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Visualization and Measurement of the Cell-Free Layer (CFL) in a Microchannel Network
... microscopy system, we have used two working fluids with different haematocrit (1% Hct and 15% Hct) and we have investigated the cell-free layer (CFL) in a microchannel network composed by asymmetric ...
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Desenvolvimento de processo de produção de fator VIII recombinante em biorreator
... rHeLa cell line than in ...to cell growth and metabolism were developed using this cell ...ep cell line couldn’t be successfully adapted to suspension growth or to serum free media, so ...
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