Eukaryotic cells
Biological Influence of Deuterium on Procariotic and Eukaryotic Cells
... d eukaryotic cells am oun ts to 8 5– 98 %, while the productivity calculated on the level of m ethan ol biocon version in to cell com pon ents m akes up 50 – 60 % ...
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Inefficient complement system clearance of Trypanosoma cruzi metacyclic trypomastigotes enables resistant strains to invade eukaryotic cells.
... The complement system is the main arm of the vertebrate innate immune system against pathogen infection. For the protozoan Trypanosoma cruzi, the causative agent of Chagas disease, subverting the complement system and ...
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A multifunctional region of the Shigella type 3 effector IpgB1 is important for secretion from bacteria and membrane targeting in eukaryotic cells.
... Type 3 secretion systems are complex nanomachines used by many Gram–negative bacteria to deliver tens of proteins (effectors) directly into host cells. Once delivered into host cells, effectors often target ...
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A novel antisense RNA from the Salmonella virulence plasmid pSLT expressed by non-growing bacteria inside eukaryotic cells.
... Figure 2. The sRNA IesR-1 is up-regulated in non-growing intracellular S. Typhimurium. Relative levels of the sRNA IesR-1 were determined by reverse transcription and RT-qPCR. Data are relative to the transcript levels ...
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Senescence marker protein 30 (SMP30) expression in eukaryotic cells: existence of multiple species and membrane localization.
... HEK-293A cells (A) and in C3A liver cells ...293A cells and required 20 times higher number in C3A liver cells since the virus does not multiply in C3A cells because these cells ...
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A new strategy for RNA isolation from eukaryotic cells using arginine affinity chromatography
... The relevance of RNA in many biological functions has been recognized, broadening the scope of RNA research activities, from basic to applied sciences, also aiming the transla- tion to clinical fields. The preparation ...
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Improved expression systems for regulated expression in Salmonella infecting eukaryotic cells.
... h cells were washed and fixed with PFA 4% during 30 min, permeabilized with ...receptors. Cells were then incubated with 1:500 anti-HA primary antibody (COVANCE) in blocking buffer ...overnight. ...
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Impact of anthropic activities on eukaryotic cells in cytotoxic test
... Numerous pollution factors generate changes in water quality and in organisms that depend on this resource, as they may contain substances with mutagenic, genotoxic and cytotoxic properties that affect the genetic ...
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The eukaryotic Pso2Snm1Artemis proteins and their function as genomic and cellular caretakers
... In yeast and mammalian cells, DSBs are predominantly repaired by one of two path- ways (1), i.e., homologous recombination (HR), or non-homologous end joining (NHEJ) (Fig- ure 1). In addition, NHEJ is also used to ...
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Effect of prostaglandin A
... Prostaglandins (PGs) are a class of natu- rally occurring cyclic 20 carbon fatty acids synthesized from polyunsaturated fatty acid precursors by most types of eukaryotic cells. These compounds have been ...
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Mutant and chimeric recobinant plasminogen activatorsproduction in eukaryotic cellsand preliminary characterization
... In the present study, we found that the recombinant proteins, produced by transfected eukaryotic cells, are efficiently secreted in the culture medium and, in most instanc[r] ...
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Dissertation presented to obtain the PhD degree in Bioengineering
... Typical 2-Cys peroxiredoxins and thioredoxin are increasingly recognized to play a central role in antioxidant protection and redox signaling in the cytoplasm of eukaryotic cells. The molecular properties ...
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Expression, purification and molecular analysis of the human ZNF706 protein
... The ZNF706 gene encodes a protein that belongs to the zinc finger family of proteins. Zinc finger proteins are in- volving in endogenous gene expression control by binding to specific DNA sequences [1]. Zinc ...
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Deep sequencing of subseafloor eukaryotic rRNA reveals active Fungi across marine subsurface provinces.
... contaminating eukaryotic cells from seawater during the sampling and drilling ...low eukaryotic biomass typical of subsurface samples [15]) introduced during RNA extraction and RT-PCR amplification ...
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Plant and fungal diversity in gut microbiota as revealed by molecular and culture investigations.
... each eukaryotic phylum and universal eukaryotic primers targeting 18S rRNA, internal transcribed spacer (ITS) and a chloroplast ...37 eukaryotic species were identified, including a Diatoms ...
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A comparison of the crystal structures of eukaryotic and bacterial SSU ribosomal RNAs reveals common structural features in the hypervariable regions.
... two eukaryotic crystal structures. The last helix in the eukaryotic V4 region was not proposed in the Gutell lab’s comparative structure but was proposed in some of the comparative structure models from ...
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Antimicrobial-induced DNA damage and genomic instability in microbial pathogens.
... other eukaryotic pathogens, such as the trypanosomal parasite ...diverse eukaryotic patho- gens, and may provide a distinctive mechanism for stress ...
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Bradysia hygida (Diptera, Sciaridae) presents two eukaryotic Elongation Factor 1A gene homologues: partial characterization of the eukaryotic Elongation Factor 1A-F1 gene
... growing cells, whereas the eEF1A-F2 gene is expressed in a developmentally regulated manner ...the cells are fully differentiated and are characterized by reduced or no cell division ...
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Biochemical Characterization and Validation of a Catalytic Site of a Highly Thermostable Ts2631 Endolysin from the Thermus scotoductus Phage vB_Tsc2631.
... The substrate specificity of the Ts2631 endolysin was tested on various Gram-positive and Gram-negative bacteria having either Orn-, Lys- or DAP- type of peptidoglycan. The results obtained are shown in Table 2. Bacteria ...
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A New Comparative-Genomics Approach for Defining Phenotype-Specific Indicators Reveals Specific Genetic Markers in Predatory Bacteria.
... as eukaryotic organisms, although phylogenetically extremely diverse, are longer than the non-predatory bacterial ones, as is reflected in their proximity in the TDO phylogenetic tree (Fig ...
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