FFPE tissue
Is high resolution melting analysis (HRMA) accurate for detection of human disease-associated mutations? A meta analysis.
... Syto9), sample source (blood cell/bone marrow, fresh frozen (FF) tissue, formalin-fixed and paraffin-embedded (FFPE) tissue or cytologic slides), eligible/non-eligible sample size (eligible (.35 ...
9
Prognostic significance of miR-205 in endometrial cancer.
... It has been reported that miR-205 targets a critical tumor suppressor gene PTEN [33,34], which is frequently mutated or deleted in endometrial cancer [4–6]. However, genetic alterations cannot account for all PTEN ...
8
Accurate data processing improves the reliability of Affymetrix gene expression profiles from FFPE samples.
... few tissue banks with frozen specimens linked to clinical meaningful information were available ...(FFPE) tissue specimens are collected for clinical routine diagnostics almost everywhere allowing a ...
10
Accuracy of quantitative polymerase chain reaction in samples of frozen and paraffin- embedded healthy skin for the diagnosis of canine visceral leishmaniasis
... in FFPE samples, the use of a larger number of sections of FFPE tissue for DNA extraction has been suggested, considering that the number of parasites per section is small (Roura et ...of FFPE ...
8
Analytical Validation of AmpliChip p53 Research Test for Archival Human Ovarian FFPE Sections.
... tumor tissue in prospec- tive clinical studies to demonstrate prognostic value of TP53 mutation status [7–8] and that treatment response correlates with TP53 mutation status in early stage breast cancer ...with ...
15
Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples
... blood, FFPE tis- sue, and FNA cells in stained slides for greater DNA ...from FFPE tissue and from long-term stored cells in stained slides (from ine-needle aspiration), which are known as scarce ...
9
One-step preservation of phosphoproteins and tissue morphology at room temperature for diagnostic and research specimens.
... Subdividing tissue, in which some tissue portions are set aside for freezing and others are used for standard formalin fixation and histology, is not desirable in the clinical diagnostic standard of care ...
19
Absolute quantitation of Met using mass spectrometry for clinical application: assay precision, stability, and correlation with MET gene amplification in FFPE tumor tissue.
... a phase I trial, we described a complete response to onartuzumab, a Met monoclonal antibody, in a patient with stage IV GEC having high MET GCN and Met over-expression [18]. A recent randomized phase II trial in GEC ...
14
A pilot study on the identi fication of human papillomavirus genotypes in tongue cancer samples from a single institution in Ecuador
... thick tissue slices of the FFPE tissue blocks, which were then placed in separate encoded tubes and ...each FFPE tissue block, using different blades, and discarding gloves and cleaning ...
5
High fidelity copy number analysis of formalin-fixed and paraffin-embedded tissues using Affymetrix Cytoscan HD chip.
... from FFPE tissue blocks are analyzed due to the fragmenting nature of genome DNA in FFPE ...from FFPE tissues practically precludes the application of whole genome copy number variation (CNV) ...
9
KLC1-ALK: a novel fusion in lung cancer identified using a formalin-fixed paraffin-embedded tissue only.
... from FFPE tissues are severely degraded during the fixation ...to FFPE tissues and identified a novel fusion, kinesin light chain 1 (KLC1)-ALK, in lung cancer by using only an FFPE ...
7
Rev. bras. farmacogn. vol.25 número6
... the tissue by an Olympus BX 40× microscope ...coral tissue generated a map recorded on a RamanScope III Bruker microscope with an Olympus BX 40× objective and a motorized stage coupled to a MultiRAM ...
8
Chemically-induced cancers do not originate from bone marrow-derived cells.
... differentiate, and resist chemotherapy and thus maintain the cancer growth. To date, the origin of CSCs remains a mystery. At least three possible origins of CSCs have been proposed, including 1) tissue-specific ...
7
J. bras. pneumol. vol.39 número6
... Table 2 summarizes the morphometric results. The proportion of alveolar septal cells immunostained for COX-1 and COX-2 was significantly higher in the UIP and NSIP tissues than in the control tissue. In other ...
9
Ideas and Perspectives: On the emission of amines from terrestrial vegetation in the context of atmospheric new particle formation
... plant tissue exhibiting high amounts of MAs might potentially lead to significant emissions, and the decomposition of organic material could constitute another source for airborne ...
33
A method for metagenomics of Helicobacter pylori from archived formalin-fixed gastric biopsies permitting longitudinal studies of carcinogenic risk.
... in FFPE 2; data not shown) and unknown (no hits, ...from tissue cell disruption during long-term storage of the biopsy at room temperature and mechanical trauma during ...
7
In-vivo fusion of human cancer and hamster stromal cells permanently transduces and transcribes human DNA.
... these FFPE tissues, which were over 40 years old, emphasizing that only positive microarray results can be considered ...the FFPE sections are of various transplant generations made over many years, and at ...
11
Multimodal phantom of liver tissue.
... temperature. Polysaccharide gels are used to prepare an inexpen- sive, conveniently moldable, and temporally stable tissue equiva- lent [14]. Using polysaccharide gels requires controlling gelling time by means of ...
9
Cell-based bone tissue engineering.
... The second published clinical study [53] describes the augmentation procedure of the posterior maxilla in 27 patients, using matrix derived from mandibular periosteum cells on a polymer fl eece (Ethisorb; Ethicon, ...
5
Liquid Crystalline Microenvironments For Tissue Engineering
... PVA is a synthetic polymer that is widely used due to its biocompatibility and ability to produce macroporous hydrogels suitable for tissue engineering [56] and drug delivery systems [57]. PVA has good elastic and ...
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