Genotyping-by-sequencing
TASSEL-GBS: a high capacity genotyping by sequencing analysis pipeline.
... generation sequencing has elicited a revolution in biology buoyed by an advancing tidal wave of raw sequence ...data[1–4]. By combining the power of next generation sequencing with reduced ...
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Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes).
... a genotyping-by-sequencing (GBS) approach to provide insights into the genetic composition of these fox ...and genotyping in other canid ...
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The population genomics of western Iberian Squalius freshwater fish species: a genotyping by sequencing approach
... In freshwater fish, processes of speciation and divergence between populations are, in many cases, ex- tremely interconnected with the geomorphology of the rivers and lakes and the formation of geological barriers that ...
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Application of genotyping-by-sequencing on semiconductor sequencing platforms: a comparison of genetic and reference-based marker ordering in barley.
... array-based genotyping as well as genotyping-by-sequencing were integrated to a framework map constructed by typing ~3000 markers in a population of 360 RILs ...
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Scanning and Filling: Ultra-Dense SNP Genotyping Combining Genotyping-By-Sequencing, SNP Array and Whole-Genome Resequencing Data.
... generation sequencing (NGS) has revolutionized plant and animal research in many ...generation sequencing has also facilitated greatly the development of methods to genotype very large numbers of molecu- ...
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New insights into adaptation and population structure of cork oak using genotyping by sequencing
... “Genotyping by Sequencing” (GBS) is used to derive 1,996 SNP markers to assess the species’ evolutionary history from a nuclear DNA perspective, gain insights on how local adaptation is shaping the ...
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Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat.
... €10. Sequencing a multiplex of 192 captured libraries on a single lane of Hiseq3000 Illumina sequencer puts the final cost of data production at about €28–30 per genotype in our ...conditions. By data ...
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Correction: A Next-Generation Sequencing Method for Genotyping-by-Sequencing of Highly Heterozygous Autotetraploid Potato.
... Uitdewilligen JGAML, Wolters A-MA, D’hoop BB, Borm TJA, Visser RGF, van Eck HJ (2013) A Next- Generation Sequencing Method for Genotyping-by-Sequencing of Highly Heterozygous Autotetrapl[r] ...
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Análise genética da resistência a Fusarium oxysporum f. sp. lactucae raça 1 em alface : aplicação de marcadores do tipo RGA e de SNPs derivados de genotyping-by-sequencing
... Fusarium wilt, caused by different races of the fungus Fusarium oxysporum f. sp. lactucae (FOLAC), is one of the most economically important diseases of lettuce (Lactuca sativa L.), in many tropical and ...
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Desenvolvimento e aplicações de DArT (Diversity Arrays Technology) e genotipagem por sequenciamento (Genotyping-by-Sequencing) para análise genética em eucalyptus
... Each individual tree in our study had a unique genotype, as would be expected from a genome-wide fingerprint comprising thousands of markers. The fact that polymorphism of DArT mark- ers relies primarily on restriction ...
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Unveiling distribution patterns of freshwater phytoplankton by a next generation sequencing based approach.
... Phytoplankton as primary producers, are directly using nutri- ents as a resource and are therefore early responders to environmental changes, making them especially suitable as eutrophication indicators. Our massive NGS ...
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DNA extraction and SSR genotyping
... SSRs were amplified using Multiplex PCR Kit (Qiagen). PCR reactions, with a final volume of 5 uL, contained 1.0 ng of DNA, 2.5 uL of 2x Qiagen multiplex PCR Master mix (HotStar Taq DNA Polymerase, PCR amplification ...
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A cost-effective approach to DNA methylation detection by Methyl Sensitive DArT sequencing.
... The earliest and probably most recognizable of the epigenetic marks is DNA methylation, that is the result of the addition of a methyl group in the 5’ position of cytosines (5mC) by sev- eral conserved and lineage ...
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Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis
... achieved by Pereira et ...caused by soybean ...balanced by the inherent short comings involved in both culture techniques and using PCR techniques to quantitatively assess changes in microbial ...
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Widespread Polycistronic Transcripts in Fungi Revealed by Single-Molecule mRNA Sequencing.
... read sequencing strategy to identify polycistronic transcripts in several mushroom forming fungal species including Plicaturopsis crispa, Phanerochaete chrysosporium, Trametes ver- sicolor, and Gloeophyllum ...
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Phylogenetics and differentiation of Salmonella Newport lineages by whole genome sequencing.
... genome sequencing data resulted in a more accurate picture of phylogeny compared to that using single genes or small sets of ...genome sequencing data enabled us to better understand the genetic background ...
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Microbiota present in cystic fibrosis lungs as revealed by whole genome sequencing.
... amplified by the primers, as demonstrated for the fungal ITSs ...genome sequencing (WGS) using the Illumina HTS technology applied directly to DNA extracted from CF respiratory clinical ...
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Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing
... The strains were identified using nearly complete se- quence of 16S rRNA gene on Ez-Taxon Server (http://eztaxon-e.ezbiocloud.net) and BLAST search on DDBJ / NCBI servers. Sequences of closely related validly published ...
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Analysis of clinical and environmental Candida parapsilosis isolates by microsatellite genotyping—a tool for hospital infection surveillance
... Analysis of clinical and environmental Candida parapsilosis isolates by microsatellite genotyping – a tool for hospital infections surveillance.. Raquel Sabino, Paula Sampaio, Laura Rosa[r] ...
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Genomic view of bipolar disorder revealed by whole genome sequencing in a genetic isolate.
... required by the Institute for Medical Research (CIMR), later Coriell - National Institute for General Medical Sciences (NIGMS) Human Genetic Cell Repository ...
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