Human regulatory T-cells
GARP is regulated by miRNAs and controls latent TGF-β1 production by human regulatory T cells.
... by T cells was thus unexpected, even more so for the following ...293 cells, because it retained the cytokine on the cell surface through disulfide bonds ...and human T ...by T ...
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Expression and function of TNF and IL-1 receptors on human regulatory T cells.
... Cells were stained in complete RPMI media or PBS+2% FCS and 0.1% sodium azide for 30 minutes at 4 uC and washed before running on BD LSR-II flow cytometer. Cell sorting was done using BD FACS Aria (BD ...
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Mycobacterium tuberculosis peptides presented by HLA-E molecules are targets for human CD8 T-cells with cytotoxic as well as regulatory activity.
... (Dynal T-cell expander beads, ...addition cells were stained for LAG-3 (17B4 kind gift of ...of cells that expressed the particular marker, markers expressed by 10% of the cells, or more, were ...
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Activation of regulatory T cells during inflammatory response is not an exclusive property of stem cells.
... stem cells (AFS) and sacrificed for specimen collection 24 h after LPS ...Arrays), T cell subpopulations (flow-cytometry) and pulmonary neutrophil influx ...homogenized ...
8
T follicular regulatory cells in human adaptive immunity and autoimmunity
... epithelial cells (SGEC) are central regulators of the autoimmune response by acting as atypical antigen-presenting cells and by constitutively expressing a plethora of immune-competent molecules, which are ...
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CD4+CD25hiCD127low regulatory T cells are increased in oral squamous cell carcinoma patients.
... York, USA). Prior to analyzing TGF-b levels, 100 m l of serum was activated by adding 4 m l of 1N HCl and incubated at room temperature for 15 minutes and neutralized using 3 m l 1N NaOH. The activated serum was then ...
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Development of regulatory T cells in the human thymus : one step beyond =desenvolvimento de células T reguladoras no timo humano
... The thymus consists of two distinct lobes joined by a connective tissue isthmus. A thin connective tissue capsule surrounds each lobe and, in most species (although not in mouse), gives rise to septa that partially ...
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Regulatory CD4+Foxp3+ T cells control the severity of anaphylaxis.
... Systemic anaphylaxis was induced in mice by i.v injection of anti-DNP IgE (10 µg) (clone SPE-7) (SIGMA-Aldrich, L’Isle d’Abeau, France). Twenty-four hours later, mice were challenged by i.v injection of 2, 4, ...
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Cord blood derived CD4+ CD25(high) T cells become functional regulatory T cells upon antigen encounter.
... Human umbilical cord blood from randomly chosen full term healthy infants (.37 weeks of gestation) was obtained by venopuncture of the umbilical vein immediately after delivery and placed in sterile sodium heparin ...
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Commensal-induced regulatory T cells mediate protection against pathogen-stimulated NF-kappaB activation.
... Treg cells by the commensal microbiota was not examined in this study but is hypothesised to be mediated in part by dendritic ...dendritic cells provide instructive signals that influence T cell ...
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Human naïve regulatory T-cells feature high steady-state turnover and are maintained by IL-7
... survival and proliferation [17]. We have previously demonstrated that IL-7-mediated naïve CD4 T-cell proliferation, but not Bcl-2 up-regulation, critically depends upon PI3K activity [17]. On the other hand, Treg ...
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Foxp3+ regulatory T cells control persistence of viral CNS infection.
... regulatory T cells (Tregs) in autoimmune and pathogen-induced immune responses has been studied intensively during recent ...exhausted T cells and to improve the immune ...
8
Eosinophils from Murine Lamina Propria Induce Differentiation of Naive T Cells into Regulatory T Cells via TGF-β1 and Retinoic Acid.
... Recombinant human TGF-β1 (Pepro- tech) at varying concentrations; and (c) RA receptor inhibitors LE540 (Wako Chemicals, Japan) and LE135 (Tocris Bioscience, UK), each at ...Treg cells in the 4 d co-cultures ...
13
In vivo SPECT reporter gene imaging of regulatory T cells.
... Retroviral supernatant was produced as previously described [19] with the following modification. The MSCV (Murine Stem Cell Virus) MIGR1 vector [22] was modified to contain human NIS followed by the IRES element ...
8
Regulatory T-cell development in the human thymus
... Regulatory T-cells develop primarily in the thymus (thymus-derived Tregs, tTregs), although they can also be differentiated in the periphery (peripherally-induced ...self-reactive T-cell ...
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Retinoic acid and rapamycin differentially affect and synergistically promote the ex vivo expansion of natural human T regulatory cells.
... K64.86 cells were resuspended in serum-free culture medium at a density 1610 6 cells/ml, and combined with human T cells (also in serum-free medium) at a final ratio of 1 ...indicated, ...
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HIV-specific T-cell responses, T-cell activation and regulatory T-cells in AIDS pathogenesis : lessons from the HIV-2 model
... + T-cell lineage devoted to immune suppression, there are so far no absolutely specific markers to identify this ...recent T-cell receptor (TCR)-mediated activation, CD25 was subsequently shown to be ...
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Ex vivo generation of human alloantigen-specific regulatory T cells from CD4(pos)CD25(high) T cells for immunotherapy.
... Involving Human Subjects Act ...pos T cells were negatively selected using mAbs directed against CD8 (RPA-T8), CD14 (M5E2), CD16 (3G8), CD19 (4G7), CD33 ...pos T cell fraction. CD25 high Treg ...
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SA-4-1BBL costimulation inhibits conversion of conventional CD4+ T cells into CD4+ FoxP3+ T regulatory cells by production of IFN-γ.
... recombinant human TGF-b1 (PeproTech, Rocky Hill, NJ) and 10 mg/mL of SA-4-1BBL or an equimolar concentration of SA protein as ...S2 cells with undetectable endotoxin levels [12]. Cells were stained ...
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Modulation of regulatory T cells in autoimmune disease
... for human AID, at the present time it is not possible to predict whether healthy individuals with benign physiological auto-reactivity or sub-clinical autoimmunity in the form of physiological auto-Ab or ...
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