K562 cells
Lapatinib induces autophagy, apoptosis and megakaryocytic differentiation in chronic myelogenous leukemia K562 cells.
... in K562 cells. After drug treatment for 3 days, cells were attached on slides using cytospin apparatus and observed microscopically after staining with Liu’s ...indicate cells with giant ...
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Knockdown of insulin receptor substrate 1 reduces proliferation and downregulates Akt/mTOR and MAPK pathways in K562 cells
... in K562 cells, we checked the activation status of Akt and P70S6K, essential proteins of the Akt/mTOR pathway, and ERK1/2, one of the MAPK ...in K562 cells resulted in a decrease of Akt, ...
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Nucleostemin depletion induces post-G1 arrest apoptosis in chronic myelogenous leukemia K562 cells
... of K562 cells were studied for various time intervals (Figure ...transfected cells, the viability of NS depleted cells was also reduced but with different kinetics (Figure ...of K562 ...
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Carbon monoxide induced erythroid differentiation of K562 cells mimics the central macrophage milieu in erythroblastic islands.
... of cells kept under air or COA. The homogeneous morphology of air-cells coincides with normal immature K562 cells, where most of cell volume is occupied by the ...mature cells in ...
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Analyzing inhibition of BCL11A gene expression in K562 cells by RNAi
... into cells by lipofectamine TM 2000 (Invitrogen, USA) solution (Dalby et ...cultured K562 cells were collected and incubated in 500 μL of cell culture medium without antibiotics and placed into each ...
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H-ferritin-regulated microRNAs modulate gene expression in K562 cells.
... the K562 erythroleukemic cell line with the aim of identifying the potentially regulated ...in K562 cells silenced for H ferritin (shFHC) versus K562 cells transduced with scrambled RNA ...
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Effects of THAP11 on erythroid differentiation and megakaryocytic differentiation of K562 cells.
... expressing K562 cells since THAP11 overexpression leads to inhibition of erythroid differentiation and accelerated megakaryo- cytic differentiation, together with reduced expression level of c- ...
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Influence of the polymorphisms of the α-major regulatory element HS-40 on in vitro gene expression
... in K562 cells were observed with the constructs containing the isolated polymorphisms +96 and +209, both located near the SP1 protein-binding sites believed not to be active in ...
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Characterization of dual effects induced by antimicrobial peptides: Regulated cell death or membrane disruption
... all cells observed fol- lowed by high amplitude swelling of the cells (Supplementary ...AMPs. K562 cells were treated with the EC 50 of each peptide for 24 h, and then ...
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A role of kindlin-3 in integrin αMβ2 outside-in signaling and the Syk-Vav1-Rac1/Cdc42 signaling axis.
... hematopoietic cells and endothelial ...transfected K562 cells that expressed endogenous kindlin-3 but not b2 ...integrins. K562 stable cell line expressing si-RNA targeting kindlin-3, but not ...
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Organopalladium compound 7b targets mitochondrial thiols and induces caspase-dependent apoptosis in human myeloid leukemia cells
... sented here pointed to compound 7b-induced apoptosis. In order to investigate whether thiol oxidation and mitochondrial depolarization were involved in the cell death, it was investigated the release of cytochrome c from ...
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The Non-Specific Binding of Fluorescent-Labeled MiRNAs on Cell Surface by Hydrophobic Interaction.
... of cells and fluorescent ...pension cells were more obvious than that of hydrophilic fluorescent dyes to adherent ...of K562 cell membrane was stronger than that of HeLa cell ...to K562 ...
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A combination of STI571 and BCR-ABL1 siRNA with overexpressed p15INK4B induced enhanced proliferation inhibition and apoptosis in chronic myeloid leukemia
... (CML) cells enhances myeloid progenitor formation from common myeloid ...CML cells. Overexpression of p15INK4B inhibited the growth of K562 cells by downregulation of cyclin-dependent kinase 4 ...
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PTEN regulates BCRP/ABCG2 and the side population through the PI3K/Akt pathway in chronic myeloid leukemia.
... stem cells named the ‘‘side population’’ (SP) has been demonstrated to be responsible for the persistence of many solid ...stem cells to targeted therapies, such as tyrosine kinase inhibitors (TKIs), ...
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BEX1 promotes imatinib-induced apoptosis by binding to and antagonizing BCL-2.
... cancer cells from undergoing apoptosis ...cancer cells to pro-apoptotic reagents including ceramide and doxorubicin ...imatinib-resistant K562 cells, whereas re-expression of BEX1 sensitized ...
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A critical role of CDKN3 in Bcr-Abl-mediated tumorigenesis.
... leukemic cells, K562 cell lines stably expressing shRNA targeting CDKN3 (sh-CDKN3) or luciferase (sh-luc) control were ...in cells expressing related shRNA (Figure 1E, and Figure ...of K562 ...
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Vorinostat induces apoptosis and differentiation in myeloid malignancies: genetic and molecular mechanisms.
... A, K562 cells were transfected with pGL3-basic vector or a series of IER3 reporters as indicated plus b-galactosidase vector, and then treated with vorinostat (2 mM) or vehicle (Control) for 24 h and ...
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Isolation of cell nuclei using inert macromolecules to mimic the crowded cytoplasm.
... from K562 cells in media containing the inert polymer 70 kDa Ficoll (50% w/v) or 70 kDa dextran (35% w/v) to replace the diffusible cytoplasmic molecules which were dispersed on cell lysis with digitonin, ...
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Braz. J. Pharm. Sci. vol.53 número1
... for K562 cells in the Trypan blue ...of cells. Although grandisin proved to be cytotoxic to K562 cells, it was also toxic for lymphocytes in the same range of concentrations ...normal ...
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J. Braz. Chem. Soc. vol.19 número1
... mononuclear cells (PBMC) and K562 cells was ...cytometry. Cells treated with metal comple- xes and -cyano-4-hydroxycinnamic acid, a known MCT inhibitor, showed recovery of viability, ...
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