Nucleic acid
Digital microfluidics for nucleic acid amplification
... Nucleic acid amplification is one of the most relevant tools in molecular biology, allowing the monitorization of gene expression, quantification of food-borne pathogens, diagnostic of hereditary and ...
13
Conjugates of Peptide Nucleic Acid-Biotin for the Detection of Bacteria using in Situ Hybridization
... This method involves the enzymatic replication of a target region of nucleic acid defined by a set of oligonucleotide primers, which are typically oriented in a convergent manner. Briefly, the PCR process ...
53
Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
... (Cartwright & Kroll 1997). Preadmission parenteral an- tibiotic is currently recommended and less lumbar punctures are done in patients with suspected meningococcal disease at presentation (Carrol et al. 2000). ...
5
Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers.
... Locked nucleic acid (LNA) is one of the most prominent and successful nucleic acid analogues because of its remarkable properties, and widely explored as building blocks in therapeutic ...
5
Discrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH)
... mimic nucleic acids have been incorporated into FISH techniques and have improved the performance of the method and substantially shortened the procedure (Stender et ...Locked nucleic acid (LNA) is ...
17
Ecophysiological traits of highly mobile large marine predators inferred from nucleic acid derived indices
... the nucleic acid-derived indices, genetic analyses to determine sex, and photographic-identification to establish residency patterns), allowed a more comprehensive analysis of the ecophysiological condition ...
10
Optimizing locked nucleic acid/2′-O-methyl-RNA fluorescence in situ hybridization (LNA/2′OMe-FISH) procedure for bacterial detection
... of nucleic acid mimics (NAMs) have emerged for in vivo and in vitro applications in the health area ...locked nucleic acid (LNA) and 2’-O-methyl-RNA (2’OMe), have been adapted for FISH ...
16
The inhibition of anti-DNA binding to DNA by nucleic acid binding polymers.
... Antibodies to DNA (anti-DNA) are the serological hallmark of systemic lupus erythematosus (SLE), a prototypic autoimmune disease characterized by the production of antibodies to components of the cell nucleus ...
7
SNBRFinder: A Sequence-Based Hybrid Algorithm for Enhanced Prediction of Nucleic Acid-Binding Residues.
... With this background, we attempted to develop a sequence-based hybrid algorithm for pre- dicting nucleic acid-binding residues. To this end, using support vector machine algorithm, we implemented a ...
23
Unraveling the potential of nucleic acid mimics to target Helicobacter pylori infections - the effect of biological barriers
... drawbacks nucleic acid mimics (NAMs) have emerged as alternatives [26, ...locked nucleic acids (LNA), 2’-OMethyl RNA (2’OMe) and phosphorothioate modified oligonucleotides (Figure ...While ...
204
In vivo activity of miR-34a mimics delivered by stable nucleic acid lipid particles (SNALPs) against multiple myeloma.
... Multiple myeloma (MM) is a disease with an adverse outcome and new therapeutic strategies are urgently awaited. A rising body of evidence supports the notion that microRNAs (miRNAs), master regulators of eukaryotic gene ...
10
Nucleic acid derived indices or instantaneous growth rate as tools to determine different nutricional condition in cuttlefish (Sepia officinalis, linnaeus 1758) hatchlings
... obtained. Nucleic acid derived indices and instantaneous growth rates (IGR) were used as a way to describe their condition, when all the yolk reserves would be exhausted and to determine the most accurate ...
7
Perspectives in isolation of microRNA from thyroid fine-needle aspiration: reply to the letter Nucleic acid recovery from thyroid fine-needle cytology slides
... of nucleic acids from long-term preserved samples ...for nucleic acid extraction, including co-extraction of RNA/DNA from thyroid FNA in stained slides samples and FFPE thyroid tissues by means of ...
2
Perspectives in isolation of microRNA from thyroid fine-needle aspiration: reply to the letter Nucleic acid recovery from thyroid fine-needle cytology slides
... of nucleic acid from the same thyroid FNA stained slides that had been previously analyzed by a cytopathologist enables an integrated diagnosis, as well as repeated FNAs, reducing the costs with ...
2
Quantitation of HIV-1 RNA Viral Load Using Nucleic Acid Sequence Based Amplification Methodology and Comparison with other Surrogate Markers for Disease Progression
... by Nucleic Acid Sequence Based Amplifica- tion (NASBA) was compared with other surrogate disease progression markers (antigen p24, CD4/CD8 cell counts and b-2 microglobulin) in 540 patients followed up at ...
6
Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of helicobacter pylori clarithromycin resistance in gastric biopsy specimens
... natively, clinical laboratories use the Etest method as it is consid- ered less technically demanding ( 21 , 22 ). However, the Etest and the agar dilution method are more tedious and time-consuming to perform than ...
8
Overlapping Patterns of Rapid Evolution in the Nucleic Acid Sensors cGAS and OAS1 Suggest a Common Mechanism of Pathogen Antagonism and Escape.
... these nucleic acid sensors have been influenced by selection from ...key nucleic acid sensors acting as a first line of host defenses against diverse ...
21
Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
... Finally, it is possible that the optimized conditions for the fixation/permeabilization proto- cols can be applicable to other microorganisms when PNA probes are used. Nonetheless, adjustments to the optimum conditions ...
13
Collaborative study to evaluate a candidate World Health Organization international standard for chikungunya virus for nucleic acid amplification technique (NAT)-based assays
... Because the candidate IS was to be assigned a unitage with respect to the CHIKV RNA content, homogeneity of the filling/freeze-drying was assessed using real-time PCR. Extraction of RNA was performed using 500 µL of the ...
45
Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsy specimens
... detects nucleic acid sequences by means of a fluorescently labeled probe that hybridizes specifically to its complementary target sequence within the intact cell ...peptide nucleic acid (PNA) ...
6