peripheral blood mononuclear cell
Peripheral blood mononuclear cell activation induced by Leptospira interrogans glycolipoprotein
... Leptospira interrogans glycolipoprotein (GLP) has been implicated in pathological and functional derange- ment seen in leptospirosis. The goal of this study was to evaluate GLP’s ability to induce cellular activation, as ...
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Quality assurance program for peripheral blood mononuclear cell Cryopreservation
... immunomagnetic cell separation (4, 5, 7, 9). While cell recovery does not interfere with the results of immunologic assays, the recovery of only a small proportion of cells may preclude assay performance ...
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Amanda Torrentes-Carvalho, Elzinandes L Azeredo, Sonia RI Reis, Alessandro S Miranda, Mariana Gandini, Luciana S Barbosa, Claire F Kubelka
... Monocytes/macrophages and dendritic cells are responsible for the dissemination of the virus after its entrance into the dermis by mosquito bite (Kou et al. 2008). Monocytes and T lymphocytes are activated during human ...
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MicroRNA expression profiles in human CD3
... T cell development, especially with regard to Tregs ...T cell transformation, reveal potential methods of programming differentiation, and produce valuable bio- marker ...the peripheral blood ...
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Nuclear NF-κB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.
... of peripheral blood mononuclear cell NF-kB p65 were higher in all diabetes groups than the control group, and were gradually elevated with the progression of type 2 diabetic nephropathy as ...
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Pharmacological Potential of Boerhaavia diffusa: An Overview
... T cell mitogen (phytohemagglutinin; PHA) stimulated proliferation of human peripheral blood mononuclear cell (PBMC), mixed lymphocyte culture, lipopolysaccharide (LPS) stimulated nitric ...
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Cytokines as determinants of resistance and pathology in human Schistosoma mansoni infection
... the peripheral blood mononuclear cell (PBMC) proliferative response and in in vitro granuloma forma- tion was evaluated in a cross-sectional study with patients with the different clinical ...
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Measles immune suppression: functional impairment or numbers game?
... Takeuchi Y, et al. (2000) Extensive lymphopenia due to apoptosis of uninfected lymphocytes in acute measles patients. Arch Virol 145: 905–920. 66. Pignata C, Fiore M, de Filippo S, Cavalcanti M, Gaetaniello L, et al. ...
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Impairment of IFN-gamma response to synthetic peptides of Mycobacterium tuberculosis in a 7-day whole blood assay.
... whole blood or peripheral blood mononuclear cell (PBMC) based assays, nor in the ELISpot ...whole blood whilst the recombinant proteins remain ...whole blood assays may ...
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Thymopoiesis and regulatory T cells in healthy children and adolescents
... peripheral blood. The quantification of TCR excision circles (TRECs) in peripheral mononuclear blood cells has been widely used as an indirect marker of recent thymus emigrant (RTE) ...
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Methods and Results: Forty biopsies from skin lesions (20 with compact granuloma – G1
... of peripheral mononuclear blood cells (PBMC) from patients with paracoccidioidomycosis (PCM) and unaffected individuals had been studied, showing significant ultrastructural differences between these ...
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Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media.
... stem cell-based therapies and fertility ...with blood-borne dis- ...Three cell lines (CHO, HEK, and C18-4), mouse embryonic stem (mES) cells, and primary cells, such as mouse embryonic fibroblast ...
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Increased activity of cell membrane-associated prothrombinase, fibrinogen-like protein 2, in peripheral blood mononuclear cells of B-cell lymphoma patients.
... Recombinant FGL-2 protein was previously shown to induce sprouting in vascular endothelial cells [18]. The coagulant activity of FGL-2 was first described in a murine fulminant hepatitis model [19]. The prothrombinase ...
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Hybrid compounds with two redox centres : modular synthesis of chalcogencontaining lapachones and studies on their antitumor activity
... cancer cell lines obtained from the National Cancer Insti- tute, NCI (Bethesda, MD, ...Janeiro Cell Bank (Rio de Janeiro, Brazil), and the Chinese hamster lung fibroblasts (V79 cells) were kindly provided by ...
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Effect of TNF- α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals
... Human T lymphotropic virus type 1 (HTLV-1) is the causal agent of myelopathy/tropical spastic paraparesis (HAM/TSP), a disease mediated by the immune response. HTLV-1 induces a spontaneous proliferation and production of ...
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Absence of peripheral blood mononuclear cells priming in hemodialysis patients
... negative and positive feedback by the cyto- kines themselves (28). In general, most cy- tokine genes are not expressed, at least at the translational level, unless specifically stimu- lated by noxious events (29). To ...
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Measurement of Peripheral Blood Mononuclear Cells Producing IFN-Gamma in Patients With Tuberculosis
... The role of cytokines produced by Th1/Th2 lymphocytes is complex due to the action of TNF-alpha, IL-12, IFN-gamma (pro-inflammatory cytokines) and IL-4, IL-10 and TGF-beta (anti-inflammatory cytokines). Th1 lymphocytes ...
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In vitro Response of the Human Breast Cancer Cell Line MDAMB-231 and Human Peripheral Blood Mononuclear Cells Exposed to
... supplemented with 10% methanol. The cells were removed using a cell-scraper (Becton Dickinson Company). The samples were washed with 5 mL of PBS. The cells were homogenized using 250 µL of lyses buffer (50 mM ...
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Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals
... nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT- PCR and the sensitivity of these methods ...
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Effect of BjcuL, a lectin isolated from Bothrops jararacussu, on human peripheral blood mononuclear cells
... incubation, cell surface labeling was performed with mono- clonal antibody PerCP anti-human CD3 or PE anti-human CD14, incubat- ed for 30 min on ice and protected from ...
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