Primary cultures
Comparison of gene expression profile in embryonic mesencephalon and neuronal primary cultures.
... (mDA) primary cultures represent a useful tool to study molecular mechanisms involved in their development and ...midbrain primary cultures enriched in mDA neurons, using microarray ...
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Responses in Primary Cultures of Mouse Cardiomyocytes Induced by Trypanosoma cruzi Trypomastigotes
... using primary cultures of embryonic mice cardiomyocytes which exhibit spontaneous contraction in ...the cultures with the protease inhibitor leupeptin did not affect the endocytic index, thus arguing ...
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Erratum for: Primary cultures of mouse small intestinal epithelial cells using the dissociating enzyme type I collagenase and hyaluronidase
... for primary culture of mouse IECs, which were isolated from the viable small intestinal crypts of murine fetuses (on embryonic day 19), using type I collagenase and hyaluronidase in a short span of time (p20 ...
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Leptin counteracts the hypoxia-induced inhibition of spontaneously firing hippocampal neurons: a microelectrode array study.
... More specifically, on DIV 11 (n = 7 MEAs), leptin markedly reduced the firing frequency by 71% with respect to controls (from 0.5460.11 Hz to 0.1660.04 Hz; p,0.01). A similar reduction has been observed also for the mean ...
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Key words: Papillomavirus; Bovine papilloma virus;
... In the present study, we obtained primary cultures from bovine wart fragments. The biop- sies were collected from affected animals with severe cutaneous papillomatosis, and/or upper ali- upper ali- mentary ...
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A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens
... The kidney is a target organ for the toxicity of several xenobiotics and is also highly susceptible to the development of malignant tumors. In both cases, in vitro studies provide insight to cellular damage, and ...
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Pseudomonas aeruginosa Reduces VX-809 Stimulated F508del-CFTR Chloride Secretion by Airway Epithelial Cells.
... In two recent studies, it was reported that VX-770 dramatically reduced the ability of VX- 809 to increase F508del-CFTR Cl secretion [22,23]. This is at odds with clinical trials with VX- 809 + VX-770 that demonstrated ...
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Sensitivity of Human Intrahepatic Cholangiocarcinoma Subtypes to Chemotherapeutics and Molecular Targeted Agents: A Study on Primary Cell Cultures.
... Also primary cultures showed large differences since CD90 + cells predominated in mucin-CCA while CD13+ cells in the mixed–CCA ...used primary cultures of mixed- and mucin-CCA to test drug ...
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Análise da expressão dos receptores de estrogênio, progesterona e EAR3 (COUP-TFI) em neoplasias epiteliais ovarianas e ovários normais
... Loss of estrogen receptor beta expression in malignant human prostate cell in primary cultures and in prostate cancer tissues. Pastorcic M, Wang H, Elbrecht A, Tsai SY, Tsai ML, O’Mall[r] ...
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Glutamate reduces glucose utilization while concomitantly enhancing AQP9 and MCT2 expression in cultured rat hippocampal neurons
... (A) Primary cultures of rat hippocampal neurons were treated with glutamate 100 µM for 1 ...different cultures with similar ...(B) Primary cultures of ...
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Identification of neural outgrowth genes using genome-wide RNAi.
... Drosophila primary neural cultures undergo a full range of developmental events starting from neuroblast division to synaptogenesis, the system also provides greater opportunity to find genes necessary for ...
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Glutamaie-evoked release of ATP from cortical astroglial cells
... astroglial cultures has always been regarded as ...in primary cultures of astroglial cells prepared from neocortex of new-born rats (Hildebrand et ...
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Angiotensin II stimulates MCP-1 production in rat glomerular endothelial cells via NAD(P)H oxidase-dependent nuclear factor-kappa B signaling
... characterized primary cultures of rat glomerular endothelial cells (GECs) and observed that Ang II induced the synthesis of monocyte chemoattractant protein-1 (MCP-1) in GECs as demonstrated by Western ...
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Glutamaie-evoked release of ATP from cortical astroglial cells
... astroglial cultures has always been regarded as ...in primary cultures of astroglial cells prepared from neocortex of new-born rats (Hildebrand et ...
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Gap junctions in hematopoietic stroma control proliferation and differentiation of blood cell precursors
... Gap junction mediated coupling, as determined by intercellular LY transfer, was in agreement with Cx43 expression (Fig. 6), showing that increased or decreased availability of Cx43, in the sense and anti-sense ...
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Rev. bras. farmacogn. vol.23 número6
... Medicinal plant propagation in vitro has been shown to be feasible for commercial production of elite plants of Passiflora. The review showed that an organogenesis-based plant regeneration system using 6-benzyladenine is ...
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Cytocidal activities of topoisomerase 1 inhibitors and 5-azacytidine against pheochromocytoma/paraganglioma cells in primary human tumor cultures and mouse cell lines.
... for cultures or ...used primary cultures of representative human tumors to establish the cytotoxicity of camptothecin, a prototypical TOP1 inhibitor, against non- dividing ...
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Host genetic factors in mouse malaria liver stage infection
... hepatocyte primary cultures we performed time course counting of infected hepatocytes that showed that less than 50% of the initial infected cells attained EEF full development (Figure ...infected ...
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Isolation, cultivation and characterization of CD133+ stem cells from human glioblastoma
... the primary culture process of the tumor, described as a homogeneous mass of spindle-shaped cells arranged in multidirectional ...these primary cultures of glioblastoma, revealed the expression of ...
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Loss of Atrx sensitizes cells to DNA damaging agents through p53-mediated death pathways.
... Here, primary macrophages isolated from Atrx f/f mice were infected with adenovirus expressing Cre recombinase or b-galactosidase, and assayed for cell survival under different experimental ...multiple ...
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